Asymmetrical Obstacles Enable Unilateral Inertial Focusing and Separation in Sinusoidal Microchannel
Inertial microfluidics uses the intrinsic fluid inertia in confined channels to manipulate the particles and cells in a simple, high-throughput, and precise manner. Inertial focusing in a straight channel results in several equilibrium positions within the cross sections. Introducing channel curvatu...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
American Association for the Advancement of Science (AAAS)
2023-01-01
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| Series: | Cyborg and Bionic Systems |
| Online Access: | https://spj.science.org/doi/10.34133/cbsystems.0036 |
| _version_ | 1797800037692997632 |
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| author | Haotian Cha Yuchen Dai Helena H. W. B. Hansen Lingxi Ouyang Xiangxun Chen Xiaoyue Kang Hongjie An Hang Thu Ta Nam-Trung Nguyen Jun Zhang |
| author_facet | Haotian Cha Yuchen Dai Helena H. W. B. Hansen Lingxi Ouyang Xiangxun Chen Xiaoyue Kang Hongjie An Hang Thu Ta Nam-Trung Nguyen Jun Zhang |
| author_sort | Haotian Cha |
| collection | DOAJ |
| description | Inertial microfluidics uses the intrinsic fluid inertia in confined channels to manipulate the particles and cells in a simple, high-throughput, and precise manner. Inertial focusing in a straight channel results in several equilibrium positions within the cross sections. Introducing channel curvature and adjusting the cross-sectional aspect ratio and shape can modify inertial focusing positions and can reduce the number of equilibrium positions. In this work, we introduce an innovative way to adjust the inertial focusing and reduce equilibrium positions by embedding asymmetrical obstacle microstructures. We demonstrated that asymmetrical concave obstacles could break the symmetry of original inertial focusing positions, resulting in unilateral focusing. In addition, we characterized the influence of obstacle size and 3 asymmetrical obstacle patterns on unilateral inertial focusing. Finally, we applied differential unilateral focusing on the separation of 10- and 15-μm particles and isolation of brain cancer cells (U87MG) from white blood cells (WBCs), respectively. The results indicated an excellent cancer cell recovery of 96.4% and WBC rejection ratio of 98.81%. After single processing, the purity of the cancer cells was dramatically enhanced from 1.01% to 90.13%, with an 89.24-fold enrichment. We believe that embedding asymmetric concave micro-obstacles is a new strategy to achieve unilateral inertial focusing and separation in curved channels. |
| first_indexed | 2024-03-13T04:29:02Z |
| format | Article |
| id | doaj.art-814b6b57e11a41a0982d70ecb0c823cd |
| institution | Directory Open Access Journal |
| issn | 2692-7632 |
| language | English |
| last_indexed | 2024-03-13T04:29:02Z |
| publishDate | 2023-01-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Cyborg and Bionic Systems |
| spelling | doaj.art-814b6b57e11a41a0982d70ecb0c823cd2023-06-19T18:46:56ZengAmerican Association for the Advancement of Science (AAAS)Cyborg and Bionic Systems2692-76322023-01-01410.34133/cbsystems.0036Asymmetrical Obstacles Enable Unilateral Inertial Focusing and Separation in Sinusoidal MicrochannelHaotian Cha0Yuchen Dai1Helena H. W. B. Hansen2Lingxi Ouyang3Xiangxun Chen4Xiaoyue Kang5Hongjie An6Hang Thu Ta7Nam-Trung Nguyen8Jun Zhang9Queensland Micro- and Nanotechnology Centre, Griffith University, Nathan, Queensland 4111, Australia.Queensland Micro- and Nanotechnology Centre, Griffith University, Nathan, Queensland 4111, Australia.Queensland Micro- and Nanotechnology Centre, Griffith University, Nathan, Queensland 4111, Australia.Queensland Micro- and Nanotechnology Centre, Griffith University, Nathan, Queensland 4111, Australia.Queensland Micro- and Nanotechnology Centre, Griffith University, Nathan, Queensland 4111, Australia.School of Engineering, University of Tasmania, Churchill Avenue, Tasmania 7005, Australia.Queensland Micro- and Nanotechnology Centre, Griffith University, Nathan, Queensland 4111, Australia.Queensland Micro- and Nanotechnology Centre, Griffith University, Nathan, Queensland 4111, Australia.Queensland Micro- and Nanotechnology Centre, Griffith University, Nathan, Queensland 4111, Australia.Queensland Micro- and Nanotechnology Centre, Griffith University, Nathan, Queensland 4111, Australia.Inertial microfluidics uses the intrinsic fluid inertia in confined channels to manipulate the particles and cells in a simple, high-throughput, and precise manner. Inertial focusing in a straight channel results in several equilibrium positions within the cross sections. Introducing channel curvature and adjusting the cross-sectional aspect ratio and shape can modify inertial focusing positions and can reduce the number of equilibrium positions. In this work, we introduce an innovative way to adjust the inertial focusing and reduce equilibrium positions by embedding asymmetrical obstacle microstructures. We demonstrated that asymmetrical concave obstacles could break the symmetry of original inertial focusing positions, resulting in unilateral focusing. In addition, we characterized the influence of obstacle size and 3 asymmetrical obstacle patterns on unilateral inertial focusing. Finally, we applied differential unilateral focusing on the separation of 10- and 15-μm particles and isolation of brain cancer cells (U87MG) from white blood cells (WBCs), respectively. The results indicated an excellent cancer cell recovery of 96.4% and WBC rejection ratio of 98.81%. After single processing, the purity of the cancer cells was dramatically enhanced from 1.01% to 90.13%, with an 89.24-fold enrichment. We believe that embedding asymmetric concave micro-obstacles is a new strategy to achieve unilateral inertial focusing and separation in curved channels.https://spj.science.org/doi/10.34133/cbsystems.0036 |
| spellingShingle | Haotian Cha Yuchen Dai Helena H. W. B. Hansen Lingxi Ouyang Xiangxun Chen Xiaoyue Kang Hongjie An Hang Thu Ta Nam-Trung Nguyen Jun Zhang Asymmetrical Obstacles Enable Unilateral Inertial Focusing and Separation in Sinusoidal Microchannel Cyborg and Bionic Systems |
| title | Asymmetrical Obstacles Enable Unilateral Inertial Focusing and Separation in Sinusoidal Microchannel |
| title_full | Asymmetrical Obstacles Enable Unilateral Inertial Focusing and Separation in Sinusoidal Microchannel |
| title_fullStr | Asymmetrical Obstacles Enable Unilateral Inertial Focusing and Separation in Sinusoidal Microchannel |
| title_full_unstemmed | Asymmetrical Obstacles Enable Unilateral Inertial Focusing and Separation in Sinusoidal Microchannel |
| title_short | Asymmetrical Obstacles Enable Unilateral Inertial Focusing and Separation in Sinusoidal Microchannel |
| title_sort | asymmetrical obstacles enable unilateral inertial focusing and separation in sinusoidal microchannel |
| url | https://spj.science.org/doi/10.34133/cbsystems.0036 |
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