Expression of Hypoxia-Inducible Factor-1α and Myoglobin in Rat Heart as Adaptive Response to Intermittent Hypobaric Hypoxia Exposure
The aim of this study was to investigate the influence of intermittent hypobaric hypoxia on the expression hypoxia adaptation proteins, namely hypoxia inducibla factor-1a (HIF-1a) and myoglobin (Mb). Twenty five male Sprague-Dawley rats were exposed to intermittent hypobaric hypoxia in a hypobaric c...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2018-03-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/20807 |
| _version_ | 1827997726072111104 |
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| author | Margaretha Herawati . Wardaya Wawan Mulyawan Fanny Septiani Farhan Frans Ferdinal Sri Widia A. Jusman Mohamad Sadikin |
| author_facet | Margaretha Herawati . Wardaya Wawan Mulyawan Fanny Septiani Farhan Frans Ferdinal Sri Widia A. Jusman Mohamad Sadikin |
| author_sort | Margaretha Herawati |
| collection | DOAJ |
| description | The aim of this study was to investigate the influence of intermittent hypobaric hypoxia on the expression hypoxia adaptation proteins, namely hypoxia inducibla factor-1a (HIF-1a) and myoglobin (Mb). Twenty five male Sprague-Dawley rats were exposed to intermittent hypobaric hypoxia in a hypobaric chamber in Indonesian Air Force Institute of Aviation Medicine, for 49.5 minutes at various low pressure, 1 week interval for 4 times (day 1, 8, 15 and 22). HIF-1α and Mb protein were measured with ELISA. mRNA expression of Mb was measured with one step real time RT-PCR. HIF-1α protein levels increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0437). mRNA expression and protein of Mb increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0283; 0.0170), and both are strongly correlated (Pearson, r = 0.6307). The heart of rats adapted to intermittent hypoxia conditions by upregulation the expression of HIF-1a and myoglobin and then both return to normal level. |
| first_indexed | 2024-04-10T05:33:32Z |
| format | Article |
| id | doaj.art-814c4c28ba10416187cf08b632bd645e |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-04-10T05:33:32Z |
| publishDate | 2018-03-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-814c4c28ba10416187cf08b632bd645e2023-03-07T02:22:11ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942018-03-0124310.4308/hjb.24.3.131Expression of Hypoxia-Inducible Factor-1α and Myoglobin in Rat Heart as Adaptive Response to Intermittent Hypobaric Hypoxia ExposureMargaretha Herawati0. Wardaya1Wawan Mulyawan2Fanny Septiani Farhan3Frans Ferdinal4Sri Widia A. Jusman5Mohamad Sadikin6Master Program in Biomedical Sciences, Faculty of Medicine University of Indonesia Department of Biochemistry, Faculty of Dentistry, Universitas Prof. Dr. Moestopo (Beragama), JakartaIndonesian Air Force Institute of Aviation Medicine (Lembaga Kesehatan Penerbangan dan Ruang Angkasa) Saryanto, JakartaIndonesian Air Force Institute of Aviation Medicine (Lembaga Kesehatan Penerbangan dan Ruang Angkasa) Saryanto, JakartaDepartment of Physiology, Faculty of Medicine Muhammadiyah University, JakartaDepartment of Biochemistry and Molecular Biology, Faculty of Medicine Tarumanagara University, JakartaCenter of Hypoxia and Oxidative Stress Studies, Faculty of Medicine University of Indonesia, Jakarta Department of Biochemistry and Molecular Biology, Faculty of Medicine University of Indonesia, JakartaCenter of Hypoxia and Oxidative Stress Studies, Faculty of Medicine University of Indonesia, Jakarta Department of Biochemistry and Molecular Biology, Faculty of Medicine University of Indonesia, JakartaThe aim of this study was to investigate the influence of intermittent hypobaric hypoxia on the expression hypoxia adaptation proteins, namely hypoxia inducibla factor-1a (HIF-1a) and myoglobin (Mb). Twenty five male Sprague-Dawley rats were exposed to intermittent hypobaric hypoxia in a hypobaric chamber in Indonesian Air Force Institute of Aviation Medicine, for 49.5 minutes at various low pressure, 1 week interval for 4 times (day 1, 8, 15 and 22). HIF-1α and Mb protein were measured with ELISA. mRNA expression of Mb was measured with one step real time RT-PCR. HIF-1α protein levels increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0437). mRNA expression and protein of Mb increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0283; 0.0170), and both are strongly correlated (Pearson, r = 0.6307). The heart of rats adapted to intermittent hypoxia conditions by upregulation the expression of HIF-1a and myoglobin and then both return to normal level.https://journal.ipb.ac.id/index.php/hayati/article/view/20807intermittent hypobaric hypoxiaHIF-1aMb |
| spellingShingle | Margaretha Herawati . Wardaya Wawan Mulyawan Fanny Septiani Farhan Frans Ferdinal Sri Widia A. Jusman Mohamad Sadikin Expression of Hypoxia-Inducible Factor-1α and Myoglobin in Rat Heart as Adaptive Response to Intermittent Hypobaric Hypoxia Exposure Hayati Journal of Biosciences intermittent hypobaric hypoxia HIF-1a Mb |
| title | Expression of Hypoxia-Inducible Factor-1α and Myoglobin in Rat Heart as Adaptive Response to Intermittent Hypobaric Hypoxia Exposure |
| title_full | Expression of Hypoxia-Inducible Factor-1α and Myoglobin in Rat Heart as Adaptive Response to Intermittent Hypobaric Hypoxia Exposure |
| title_fullStr | Expression of Hypoxia-Inducible Factor-1α and Myoglobin in Rat Heart as Adaptive Response to Intermittent Hypobaric Hypoxia Exposure |
| title_full_unstemmed | Expression of Hypoxia-Inducible Factor-1α and Myoglobin in Rat Heart as Adaptive Response to Intermittent Hypobaric Hypoxia Exposure |
| title_short | Expression of Hypoxia-Inducible Factor-1α and Myoglobin in Rat Heart as Adaptive Response to Intermittent Hypobaric Hypoxia Exposure |
| title_sort | expression of hypoxia inducible factor 1α and myoglobin in rat heart as adaptive response to intermittent hypobaric hypoxia exposure |
| topic | intermittent hypobaric hypoxia HIF-1a Mb |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/20807 |
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