Antiparasitic activity of Astragalus brachycalyx subsp. brachycalyx extract against hydatid cyst protoscoleces and its effect on induction of apoptosis: an in vitro and ex vivo study
Introduction: This study aims to evaluate in vitro and ex vivo antiparasitic activity of Astragalus brachycalyx subsp. brachycalyx. root chloroformic extract against hydatid cyst protoscoleces and its effect on induction of apoptosis. Methods: Various concentrations of the A. brachycalyx root chloro...
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Shahrekord University of Medical Sciences
2022-07-01
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Series: | Journal of HerbMed Pharmacology |
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Online Access: | http://herbmedpharmacol.com/PDF/jhp-11-428.pdf |
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author | Javad Ghasemian Yadegari Amal Khudair Khalaf Massumeh Saadatmand Hossein Mahmoudvand |
author_facet | Javad Ghasemian Yadegari Amal Khudair Khalaf Massumeh Saadatmand Hossein Mahmoudvand |
author_sort | Javad Ghasemian Yadegari |
collection | DOAJ |
description | Introduction: This study aims to evaluate in vitro and ex vivo antiparasitic activity of Astragalus brachycalyx subsp. brachycalyx. root chloroformic extract against hydatid cyst protoscoleces and its effect on induction of apoptosis. Methods: Various concentrations of the A. brachycalyx root chloroformic extract (56.25, 112.5, 225, and 450 mg/mL) were treated with hydatid cyst protoscoleces collected from the liver of infected sheep for 5-60minutes in vitro and ex vivo. Eosin exclusion test was also utilized to measure the mortality of protoscoleces. Moreover, the extract effect was assessed on apoptosis induction in hydatid cyst protoscoleces by caspase-3 activity measurement. Results: The mortality rate of protoscoleces in in vitro was 100% after being exposed to 450 and 225 mg/mL of A. brachycalyx extract for 20 and 30 minutes and in ex vivo for 30 and 60 minutes, respectively. Following 48 h treatment of protoscoleces, A. brachycalyx chloroformic extract at the doses of 56.25, 112.5, 225, and 450 mg/mL, dose-dependently motivated the caspase-3 enzyme ranging from 8.8% to 29.6% Conclusion: A. brachycalyx root chloroformic extract had a significant protoscolicidal effect; however, extra surveys are required to assess its efficacy and safety as a promising protoscolicidal agent in clinical settings. |
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language | English |
last_indexed | 2024-04-13T09:31:12Z |
publishDate | 2022-07-01 |
publisher | Shahrekord University of Medical Sciences |
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series | Journal of HerbMed Pharmacology |
spelling | doaj.art-814f236517504c4eb69b8e33877616d32022-12-22T02:52:14ZengShahrekord University of Medical SciencesJournal of HerbMed Pharmacology2345-50042022-07-0111342843410.34172/jhp.2022.49jhp-42414Antiparasitic activity of Astragalus brachycalyx subsp. brachycalyx extract against hydatid cyst protoscoleces and its effect on induction of apoptosis: an in vitro and ex vivo studyJavad Ghasemian Yadegari0Amal Khudair Khalaf1Massumeh Saadatmand2Hossein Mahmoudvand3Department of Pharmacognosy, School of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, IranDepartment of Microbiology, College of Medicine, University of Thiqar, IraqStudent Research Committee, Lorestan University of Medical Sciences, Khorramabad, IranRazi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IranIntroduction: This study aims to evaluate in vitro and ex vivo antiparasitic activity of Astragalus brachycalyx subsp. brachycalyx. root chloroformic extract against hydatid cyst protoscoleces and its effect on induction of apoptosis. Methods: Various concentrations of the A. brachycalyx root chloroformic extract (56.25, 112.5, 225, and 450 mg/mL) were treated with hydatid cyst protoscoleces collected from the liver of infected sheep for 5-60minutes in vitro and ex vivo. Eosin exclusion test was also utilized to measure the mortality of protoscoleces. Moreover, the extract effect was assessed on apoptosis induction in hydatid cyst protoscoleces by caspase-3 activity measurement. Results: The mortality rate of protoscoleces in in vitro was 100% after being exposed to 450 and 225 mg/mL of A. brachycalyx extract for 20 and 30 minutes and in ex vivo for 30 and 60 minutes, respectively. Following 48 h treatment of protoscoleces, A. brachycalyx chloroformic extract at the doses of 56.25, 112.5, 225, and 450 mg/mL, dose-dependently motivated the caspase-3 enzyme ranging from 8.8% to 29.6% Conclusion: A. brachycalyx root chloroformic extract had a significant protoscolicidal effect; however, extra surveys are required to assess its efficacy and safety as a promising protoscolicidal agent in clinical settings.http://herbmedpharmacol.com/PDF/jhp-11-428.pdfhydatid cystechinococcus granulosusscolicidal activitycaspaseherbal medicine |
spellingShingle | Javad Ghasemian Yadegari Amal Khudair Khalaf Massumeh Saadatmand Hossein Mahmoudvand Antiparasitic activity of Astragalus brachycalyx subsp. brachycalyx extract against hydatid cyst protoscoleces and its effect on induction of apoptosis: an in vitro and ex vivo study Journal of HerbMed Pharmacology hydatid cyst echinococcus granulosus scolicidal activity caspase herbal medicine |
title | Antiparasitic activity of Astragalus brachycalyx subsp. brachycalyx extract against hydatid cyst protoscoleces and its effect on induction of apoptosis: an in vitro and ex vivo study |
title_full | Antiparasitic activity of Astragalus brachycalyx subsp. brachycalyx extract against hydatid cyst protoscoleces and its effect on induction of apoptosis: an in vitro and ex vivo study |
title_fullStr | Antiparasitic activity of Astragalus brachycalyx subsp. brachycalyx extract against hydatid cyst protoscoleces and its effect on induction of apoptosis: an in vitro and ex vivo study |
title_full_unstemmed | Antiparasitic activity of Astragalus brachycalyx subsp. brachycalyx extract against hydatid cyst protoscoleces and its effect on induction of apoptosis: an in vitro and ex vivo study |
title_short | Antiparasitic activity of Astragalus brachycalyx subsp. brachycalyx extract against hydatid cyst protoscoleces and its effect on induction of apoptosis: an in vitro and ex vivo study |
title_sort | antiparasitic activity of astragalus brachycalyx subsp brachycalyx extract against hydatid cyst protoscoleces and its effect on induction of apoptosis an in vitro and ex vivo study |
topic | hydatid cyst echinococcus granulosus scolicidal activity caspase herbal medicine |
url | http://herbmedpharmacol.com/PDF/jhp-11-428.pdf |
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