Effects of handheld nonthermal plasma on the biological responses, mineralization, and inflammatory reactions of polyaryletherketone implant materials
Background/purpose: The handheld nonthermal plasma (HNP) treatment may alter the surface properties, bone metabolism, and inflammatory reactions of polyaryletherketone (PAEK) dental implant materials. This study tested whether the HNP treatment might increase the biocompatibility, surface hydrophili...
| Príomhchruthaitheoirí: | , , , , , , |
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| Formáid: | Alt |
| Teanga: | English |
| Foilsithe / Cruthaithe: |
Elsevier
2024-10-01
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| Sraith: | Journal of Dental Sciences |
| Ábhair: | |
| Rochtain ar líne: | http://www.sciencedirect.com/science/article/pii/S1991790224002113 |
| _version_ | 1827116737805418496 |
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| author | Chien-Fu Tseng I-Ta Lee Sheng-Han Wu Hsin-Ming Chen Yuichi Mine Tzu-Yu Peng Sang-Heng Kok |
| author_facet | Chien-Fu Tseng I-Ta Lee Sheng-Han Wu Hsin-Ming Chen Yuichi Mine Tzu-Yu Peng Sang-Heng Kok |
| author_sort | Chien-Fu Tseng |
| collection | DOAJ |
| description | Background/purpose: The handheld nonthermal plasma (HNP) treatment may alter the surface properties, bone metabolism, and inflammatory reactions of polyaryletherketone (PAEK) dental implant materials. This study tested whether the HNP treatment might increase the biocompatibility, surface hydrophilicity, surface free energies (SFEs), and the cell adhesion and mineralization capability of PAEK materials. Materials and methods: Disk-shaped samples of titanium (Ti), zirconia (Zr), polyetheretherketone (PEEK [PE]), and polyetherketoneketone (PEKK [PK]) were subjected to HNP treatment and termed as TiPL, ZrPL, PEPL, and PKPL, respectively. Water-surface reactions were examined using a goniometer. MG-63 cells were cultured on all samples to assess the cell viability, cytotoxicity, cell attachment, and mineralization characteristics. The expression of pro-inflammatory cytokines (tumor necrosis factor-alpha and interleukin-6) and key mineralization markers (alkaline phosphatase [ALKP], osteopontin [OPN], and dentin matrix protein 1 [DMP1]) was measured using enzyme-linked immunosorbent assay kits. Results: The HNP-treated samples exhibited significantly enhanced surface hydrophilicities and SFEs compared to the untreated samples. The cell viability remained high across all samples, indicating no cytotoxic effects. The HNP treatment significantly enhanced MG-63 cell adherence and proliferation. Elevated levels of ALKP and OPN were observed for the plasma-treated PEPL and PKPL specimens, while DMP1 levels increased significantly only in the PKPL specimen. Pro-inflammatory cytokine levels were low across all samples, suggesting no inflammatory response. Conclusion: The HNP-treated PAEKs have enhanced the surface hydrophilicity and SFEs as well as superior cell adhesion and mineralization capability, and thus may be good clinical dental implant materials. |
| first_indexed | 2025-03-20T12:38:25Z |
| format | Article |
| id | doaj.art-814f8cd87b73401a903161f5637ee57a |
| institution | Directory Open Access Journal |
| issn | 1991-7902 |
| language | English |
| last_indexed | 2025-03-20T12:38:25Z |
| publishDate | 2024-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dental Sciences |
| spelling | doaj.art-814f8cd87b73401a903161f5637ee57a2024-09-13T04:35:58ZengElsevierJournal of Dental Sciences1991-79022024-10-0119420182026Effects of handheld nonthermal plasma on the biological responses, mineralization, and inflammatory reactions of polyaryletherketone implant materialsChien-Fu Tseng0I-Ta Lee1Sheng-Han Wu2Hsin-Ming Chen3Yuichi Mine4Tzu-Yu Peng5Sang-Heng Kok6Graduate Institute of Clinical Dentistry, School of Dentistry, College of Medicine, National Taiwan University, Taipei, Taiwan; Department of Dentistry, Taoyunan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan; Research Center of Precision Biomedical Implants, College of Oral Medicine, Taipei Medical University, Taipei, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, TaiwanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, TaiwanGraduate Institute of Clinical Dentistry, School of Dentistry, College of Medicine, National Taiwan University, Taipei, Taiwan; Graduate Institute of Oral Biology, School of Dentistry, College of Medicine, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, TaiwanDepartment of Medical Systems Engineering, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan; Project Research Center for Integrating Digital Dentistry, Hiroshima University, Hiroshima, JapanSchool of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; Corresponding author. School of Dentistry, College of Oral Medicine, Taipei Medical University, 250 Wu-Hsing Street, Taipei, 110, Taiwan.Graduate Institute of Clinical Dentistry, School of Dentistry, College of Medicine, National Taiwan University, Taipei, Taiwan; Department of Dentistry, Taoyunan General Hospital, Ministry of Health and Welfare, Taoyuan, Taiwan; Department of Dentistry, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan; Corresponding author. Department of Dentistry, School of Dentistry, College of Medicine, National Taiwan University, No. 1, Changde Street, Taipei, 10048, Taiwan.Background/purpose: The handheld nonthermal plasma (HNP) treatment may alter the surface properties, bone metabolism, and inflammatory reactions of polyaryletherketone (PAEK) dental implant materials. This study tested whether the HNP treatment might increase the biocompatibility, surface hydrophilicity, surface free energies (SFEs), and the cell adhesion and mineralization capability of PAEK materials. Materials and methods: Disk-shaped samples of titanium (Ti), zirconia (Zr), polyetheretherketone (PEEK [PE]), and polyetherketoneketone (PEKK [PK]) were subjected to HNP treatment and termed as TiPL, ZrPL, PEPL, and PKPL, respectively. Water-surface reactions were examined using a goniometer. MG-63 cells were cultured on all samples to assess the cell viability, cytotoxicity, cell attachment, and mineralization characteristics. The expression of pro-inflammatory cytokines (tumor necrosis factor-alpha and interleukin-6) and key mineralization markers (alkaline phosphatase [ALKP], osteopontin [OPN], and dentin matrix protein 1 [DMP1]) was measured using enzyme-linked immunosorbent assay kits. Results: The HNP-treated samples exhibited significantly enhanced surface hydrophilicities and SFEs compared to the untreated samples. The cell viability remained high across all samples, indicating no cytotoxic effects. The HNP treatment significantly enhanced MG-63 cell adherence and proliferation. Elevated levels of ALKP and OPN were observed for the plasma-treated PEPL and PKPL specimens, while DMP1 levels increased significantly only in the PKPL specimen. Pro-inflammatory cytokine levels were low across all samples, suggesting no inflammatory response. Conclusion: The HNP-treated PAEKs have enhanced the surface hydrophilicity and SFEs as well as superior cell adhesion and mineralization capability, and thus may be good clinical dental implant materials.http://www.sciencedirect.com/science/article/pii/S1991790224002113Handheld nonthermal plasmaDental implantPolyaryletherketoneCell metabolic activityMineralizationInflammatory reaction |
| spellingShingle | Chien-Fu Tseng I-Ta Lee Sheng-Han Wu Hsin-Ming Chen Yuichi Mine Tzu-Yu Peng Sang-Heng Kok Effects of handheld nonthermal plasma on the biological responses, mineralization, and inflammatory reactions of polyaryletherketone implant materials Journal of Dental Sciences Handheld nonthermal plasma Dental implant Polyaryletherketone Cell metabolic activity Mineralization Inflammatory reaction |
| title | Effects of handheld nonthermal plasma on the biological responses, mineralization, and inflammatory reactions of polyaryletherketone implant materials |
| title_full | Effects of handheld nonthermal plasma on the biological responses, mineralization, and inflammatory reactions of polyaryletherketone implant materials |
| title_fullStr | Effects of handheld nonthermal plasma on the biological responses, mineralization, and inflammatory reactions of polyaryletherketone implant materials |
| title_full_unstemmed | Effects of handheld nonthermal plasma on the biological responses, mineralization, and inflammatory reactions of polyaryletherketone implant materials |
| title_short | Effects of handheld nonthermal plasma on the biological responses, mineralization, and inflammatory reactions of polyaryletherketone implant materials |
| title_sort | effects of handheld nonthermal plasma on the biological responses mineralization and inflammatory reactions of polyaryletherketone implant materials |
| topic | Handheld nonthermal plasma Dental implant Polyaryletherketone Cell metabolic activity Mineralization Inflammatory reaction |
| url | http://www.sciencedirect.com/science/article/pii/S1991790224002113 |
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