Glycogen Synthase Kinase 3 Gene Is Important in Growth and Molting of the Pacific White Shrimp Litopenaeus vannamei

Glycogen synthase kinase 3 (GSK3) is a vital multifunctional molecule that is widely distributed in invertebrates and vertebrates. GSK3 is a highly conserved serine/threonine (Ser/Thr) protein kinase, which plays an important role in insulin, Wnt, and various signaling pathways. In this study, a GSK...

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Main Authors: Ying Pang, Xiaojun Zhang, Jianbo Yuan, Xiaoxi Zhang, Manwen Su, Fuhua Li
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-06-01
Series:Frontiers in Marine Science
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fmars.2021.681966/full
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author Ying Pang
Ying Pang
Ying Pang
Xiaojun Zhang
Xiaojun Zhang
Xiaojun Zhang
Jianbo Yuan
Jianbo Yuan
Jianbo Yuan
Xiaoxi Zhang
Xiaoxi Zhang
Manwen Su
Manwen Su
Fuhua Li
Fuhua Li
Fuhua Li
author_facet Ying Pang
Ying Pang
Ying Pang
Xiaojun Zhang
Xiaojun Zhang
Xiaojun Zhang
Jianbo Yuan
Jianbo Yuan
Jianbo Yuan
Xiaoxi Zhang
Xiaoxi Zhang
Manwen Su
Manwen Su
Fuhua Li
Fuhua Li
Fuhua Li
author_sort Ying Pang
collection DOAJ
description Glycogen synthase kinase 3 (GSK3) is a vital multifunctional molecule that is widely distributed in invertebrates and vertebrates. GSK3 is a highly conserved serine/threonine (Ser/Thr) protein kinase, which plays an important role in insulin, Wnt, and various signaling pathways. In this study, a GSK3 gene were identified in the genome of the Pacific white shrimp, Litopenaeus vannamei, and analyzed its gene structure, phylogeny, and expression profiles. The deduced LvGSK3 protein contains a highly conserved Ser/Thr protein kinase catalytic (S_TKc) domain, the LvGSK3 gene exhibited high expression in different early developmental stages, most adult tissues, and premolting stages. RNA interference of LvGSK3 significantly retarded the increment of body weight and affected the expressions of molting-related genes compared with control groups. These results will improve our understanding of the conserved structure and functions of the LvGSK3 gene and show potential applications of shrimp growth.
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spelling doaj.art-81500428f2d04e1f95552bff514cd9a42022-12-21T21:59:09ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452021-06-01810.3389/fmars.2021.681966681966Glycogen Synthase Kinase 3 Gene Is Important in Growth and Molting of the Pacific White Shrimp Litopenaeus vannameiYing Pang0Ying Pang1Ying Pang2Xiaojun Zhang3Xiaojun Zhang4Xiaojun Zhang5Jianbo Yuan6Jianbo Yuan7Jianbo Yuan8Xiaoxi Zhang9Xiaoxi Zhang10Manwen Su11Manwen Su12Fuhua Li13Fuhua Li14Fuhua Li15CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaCollege of Earth Science, University of Chinese Academy of Sciences, Beijing, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaCollege of Earth Science, University of Chinese Academy of Sciences, Beijing, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaCollege of Earth Science, University of Chinese Academy of Sciences, Beijing, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaCollege of Earth Science, University of Chinese Academy of Sciences, Beijing, ChinaCAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, ChinaCollege of Earth Science, University of Chinese Academy of Sciences, Beijing, ChinaGlycogen synthase kinase 3 (GSK3) is a vital multifunctional molecule that is widely distributed in invertebrates and vertebrates. GSK3 is a highly conserved serine/threonine (Ser/Thr) protein kinase, which plays an important role in insulin, Wnt, and various signaling pathways. In this study, a GSK3 gene were identified in the genome of the Pacific white shrimp, Litopenaeus vannamei, and analyzed its gene structure, phylogeny, and expression profiles. The deduced LvGSK3 protein contains a highly conserved Ser/Thr protein kinase catalytic (S_TKc) domain, the LvGSK3 gene exhibited high expression in different early developmental stages, most adult tissues, and premolting stages. RNA interference of LvGSK3 significantly retarded the increment of body weight and affected the expressions of molting-related genes compared with control groups. These results will improve our understanding of the conserved structure and functions of the LvGSK3 gene and show potential applications of shrimp growth.https://www.frontiersin.org/articles/10.3389/fmars.2021.681966/fullglycogen synthase kinase 3Litopenaeus vannameigrowthmoltingstructure and function
spellingShingle Ying Pang
Ying Pang
Ying Pang
Xiaojun Zhang
Xiaojun Zhang
Xiaojun Zhang
Jianbo Yuan
Jianbo Yuan
Jianbo Yuan
Xiaoxi Zhang
Xiaoxi Zhang
Manwen Su
Manwen Su
Fuhua Li
Fuhua Li
Fuhua Li
Glycogen Synthase Kinase 3 Gene Is Important in Growth and Molting of the Pacific White Shrimp Litopenaeus vannamei
Frontiers in Marine Science
glycogen synthase kinase 3
Litopenaeus vannamei
growth
molting
structure and function
title Glycogen Synthase Kinase 3 Gene Is Important in Growth and Molting of the Pacific White Shrimp Litopenaeus vannamei
title_full Glycogen Synthase Kinase 3 Gene Is Important in Growth and Molting of the Pacific White Shrimp Litopenaeus vannamei
title_fullStr Glycogen Synthase Kinase 3 Gene Is Important in Growth and Molting of the Pacific White Shrimp Litopenaeus vannamei
title_full_unstemmed Glycogen Synthase Kinase 3 Gene Is Important in Growth and Molting of the Pacific White Shrimp Litopenaeus vannamei
title_short Glycogen Synthase Kinase 3 Gene Is Important in Growth and Molting of the Pacific White Shrimp Litopenaeus vannamei
title_sort glycogen synthase kinase 3 gene is important in growth and molting of the pacific white shrimp litopenaeus vannamei
topic glycogen synthase kinase 3
Litopenaeus vannamei
growth
molting
structure and function
url https://www.frontiersin.org/articles/10.3389/fmars.2021.681966/full
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