Structural Characterization and Immunobiological Activity of Polysaccharides from Astragalus Oyster Mushroom
When added to mushroom growing substrates, edible and medicinal herbs affect the mushrooms’ nutritional and medicinal value. In this study, polysaccharides (P<sup>0</sup>OP-I and P<sup>15</sup>OP-I) were extracted and purified from oyster mushrooms grown on substrates supplem...
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MDPI AG
2023-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/13/5280 |
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| author | Qiuxia Meng Yu Niu Rongrong Wang Wei Niu Lizhen Zhang |
| author_facet | Qiuxia Meng Yu Niu Rongrong Wang Wei Niu Lizhen Zhang |
| author_sort | Qiuxia Meng |
| collection | DOAJ |
| description | When added to mushroom growing substrates, edible and medicinal herbs affect the mushrooms’ nutritional and medicinal value. In this study, polysaccharides (P<sup>0</sup>OP-I and P<sup>15</sup>OP-I) were extracted and purified from oyster mushrooms grown on substrates supplemented with 0% and 15% Astragalus roots (P<sup>0</sup> and P<sup>15</sup>), respectively, and their chemical structure and immunobiological activities were compared. P<sup>15</sup>OP-I and P<sup>0</sup>OP-I were extracted using ultrasound-assisted hot water and deproteinized with the Sevage method, depigmented with 30% H<sub>2</sub>O<sub>2</sub>, desalted with dialysis, and purified using DEAE-52 cellulose and Sephadex G-100 dextran column chromatography. The molecular weight of P<sup>0</sup>OP-I and P<sup>15</sup>OP-I was 21,706.96 and 20,172.65 Da, respectively. Both were composed of monosaccharides <i>D</i>-mannose, galacturonic acid, <i>D</i>-glucose, <i>D</i>-galactose, and <i>L</i>-arabinose but in different molar ratios, and both were connected by a pyranoside linkage. P<sup>15</sup>OP-I consisted of higher contents of mannose, glucose, galactose and arabinose and lower content of galacturonic acid as compared to P<sup>0</sup>OP-I. Both P<sup>0</sup>OP-I and P<sup>15</sup>OP-I induced NO and TNF-α production but did not show cytotoxic effect or induce ROS generation in RAW264.7 cells. P<sup>15</sup>OP-I showed a stronger ability to promote NO and TNF-α production relative to P<sup>0</sup>OP-I. In vitro experiments showed that the immunomodulatory activity of P<sup>0</sup>OP-I and P<sup>15</sup>OP-I in RAW264.7 macrophages were mediated by the JNK/MAPK, Erk/MAPK, and NF-κB signaling pathways. The results would be helpful for elucidation of the health promoting mechanism of Astragalus oyster mushrooms as a source of neutraceuticals. |
| first_indexed | 2024-03-11T01:33:20Z |
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| id | doaj.art-8159cbe69108432abb4d43fd2effb11b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T01:33:20Z |
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| spelling | doaj.art-8159cbe69108432abb4d43fd2effb11b2023-11-18T17:10:50ZengMDPI AGMolecules1420-30492023-07-012813528010.3390/molecules28135280Structural Characterization and Immunobiological Activity of Polysaccharides from Astragalus Oyster MushroomQiuxia Meng0Yu Niu1Rongrong Wang2Wei Niu3Lizhen Zhang4Institute of Eco-environment and Industrial Technology, Shanxi Agricultural University, Taiyuan 030031, ChinaCollege of Agricultural Economics and Management, Shanxi Agricultural University, Taiyuan 030006, ChinaSchool of Life Sciences, Shanxi University, Taiyuan 030006, ChinaFinancial and Assets Department, Shanxi Agricultural University, Taigu 030801, ChinaSchool of Life Sciences, Shanxi University, Taiyuan 030006, ChinaWhen added to mushroom growing substrates, edible and medicinal herbs affect the mushrooms’ nutritional and medicinal value. In this study, polysaccharides (P<sup>0</sup>OP-I and P<sup>15</sup>OP-I) were extracted and purified from oyster mushrooms grown on substrates supplemented with 0% and 15% Astragalus roots (P<sup>0</sup> and P<sup>15</sup>), respectively, and their chemical structure and immunobiological activities were compared. P<sup>15</sup>OP-I and P<sup>0</sup>OP-I were extracted using ultrasound-assisted hot water and deproteinized with the Sevage method, depigmented with 30% H<sub>2</sub>O<sub>2</sub>, desalted with dialysis, and purified using DEAE-52 cellulose and Sephadex G-100 dextran column chromatography. The molecular weight of P<sup>0</sup>OP-I and P<sup>15</sup>OP-I was 21,706.96 and 20,172.65 Da, respectively. Both were composed of monosaccharides <i>D</i>-mannose, galacturonic acid, <i>D</i>-glucose, <i>D</i>-galactose, and <i>L</i>-arabinose but in different molar ratios, and both were connected by a pyranoside linkage. P<sup>15</sup>OP-I consisted of higher contents of mannose, glucose, galactose and arabinose and lower content of galacturonic acid as compared to P<sup>0</sup>OP-I. Both P<sup>0</sup>OP-I and P<sup>15</sup>OP-I induced NO and TNF-α production but did not show cytotoxic effect or induce ROS generation in RAW264.7 cells. P<sup>15</sup>OP-I showed a stronger ability to promote NO and TNF-α production relative to P<sup>0</sup>OP-I. In vitro experiments showed that the immunomodulatory activity of P<sup>0</sup>OP-I and P<sup>15</sup>OP-I in RAW264.7 macrophages were mediated by the JNK/MAPK, Erk/MAPK, and NF-κB signaling pathways. The results would be helpful for elucidation of the health promoting mechanism of Astragalus oyster mushrooms as a source of neutraceuticals.https://www.mdpi.com/1420-3049/28/13/5280Hengshan Astragalus oyster mushroompolysaccharideimmunomodulation |
| spellingShingle | Qiuxia Meng Yu Niu Rongrong Wang Wei Niu Lizhen Zhang Structural Characterization and Immunobiological Activity of Polysaccharides from Astragalus Oyster Mushroom Molecules Hengshan Astragalus oyster mushroom polysaccharide immunomodulation |
| title | Structural Characterization and Immunobiological Activity of Polysaccharides from Astragalus Oyster Mushroom |
| title_full | Structural Characterization and Immunobiological Activity of Polysaccharides from Astragalus Oyster Mushroom |
| title_fullStr | Structural Characterization and Immunobiological Activity of Polysaccharides from Astragalus Oyster Mushroom |
| title_full_unstemmed | Structural Characterization and Immunobiological Activity of Polysaccharides from Astragalus Oyster Mushroom |
| title_short | Structural Characterization and Immunobiological Activity of Polysaccharides from Astragalus Oyster Mushroom |
| title_sort | structural characterization and immunobiological activity of polysaccharides from astragalus oyster mushroom |
| topic | Hengshan Astragalus oyster mushroom polysaccharide immunomodulation |
| url | https://www.mdpi.com/1420-3049/28/13/5280 |
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