CLONING AND SEQUENCING OF PGIP FROM ‘JIN SERIES’ ALMOND (PRUNUS DULCIS)
Specific primers synthesized according to conservative regions of polygalacturonase inhibiting protein (PGIP) gene were used to amplify Prunus Dulcis genomic DNA by polymerase-chain reaction (PCR). Six bands (pgip1, pgip2, pgip3, pgip4, pgip5 and pgip6) of genes were obtained and cloned into PBS-...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Research Institute for Fruit Growing Pitesti
2015-12-01
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| Series: | Fruit Growing Research |
| Subjects: | |
| Online Access: | http://publications.icdp.ro/publicatii/lucrari%202015/02.Han%20and%20Tian%20China.pdf |
| _version_ | 1818516367854272512 |
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| author | Yuhu Han Ge Tian Yanxia Wu Yiwen Ji Qiufen Cao Jian-bao Tian2 |
| author_facet | Yuhu Han Ge Tian Yanxia Wu Yiwen Ji Qiufen Cao Jian-bao Tian2 |
| author_sort | Yuhu Han |
| collection | DOAJ |
| description | Specific primers synthesized according to conservative regions of polygalacturonase
inhibiting protein (PGIP) gene were used to amplify Prunus Dulcis genomic DNA by
polymerase-chain reaction (PCR). Six bands (pgip1, pgip2, pgip3, pgip4, pgip5 and pgip6) of genes
were obtained and cloned into PBS-T vector. According to the length of bands, 717bp, 864bp,
796bp were A1 (pgip1, pgip2, pgip3), A2 (pgip4), A4 (pgip5, pgip6), respectively. DNA sequences
showed that the fragments taken together were the gene encoding PGIP. A2 and A3 contained two
exons interrupted by one intron, which has GT-AG sequence. Its DNA and amino acid sequences
were highly homologies to those from Prunus Persica; Prunus Salicina; Prunus Americana;
Prunus Mume, respectively. A conserved lencinerial fragment exists in the derived protein
sequence. |
| first_indexed | 2024-12-11T00:41:07Z |
| format | Article |
| id | doaj.art-815c70c2c11e4832be4628d7c440fcc8 |
| institution | Directory Open Access Journal |
| issn | 2286-0304 |
| language | English |
| last_indexed | 2024-12-11T00:41:07Z |
| publishDate | 2015-12-01 |
| publisher | Research Institute for Fruit Growing Pitesti |
| record_format | Article |
| series | Fruit Growing Research |
| spelling | doaj.art-815c70c2c11e4832be4628d7c440fcc82022-12-22T01:26:55ZengResearch Institute for Fruit Growing PitestiFruit Growing Research2286-03042015-12-01XXXI1722CLONING AND SEQUENCING OF PGIP FROM ‘JIN SERIES’ ALMOND (PRUNUS DULCIS)Yuhu Han0Ge Tian1Yanxia Wu2Yiwen Ji3Qiufen Cao4Jian-bao Tian25Institute of Pomology, Shanxi Academy of Agricultural Sciences, Taigu, Shanxi 030815 China Shanxi Academy of Agricultural Sciences, Longcheng St 81, Taiyuan, Shanxi 030031 China Institute of Pomology, Shanxi Academy of Agricultural Sciences, Taigu, Shanxi 030815 China Institute of Pomology, Shanxi Academy of Agricultural Sciences, Taigu, Shanxi 030815 China Shanxi Academy of Agricultural Sciences, Longcheng St 81, Taiyuan, Shanxi 030031 China Shanxi Academy of Agricultural Sciences, Longcheng St 81, Taiyuan, Shanxi 030031 China Specific primers synthesized according to conservative regions of polygalacturonase inhibiting protein (PGIP) gene were used to amplify Prunus Dulcis genomic DNA by polymerase-chain reaction (PCR). Six bands (pgip1, pgip2, pgip3, pgip4, pgip5 and pgip6) of genes were obtained and cloned into PBS-T vector. According to the length of bands, 717bp, 864bp, 796bp were A1 (pgip1, pgip2, pgip3), A2 (pgip4), A4 (pgip5, pgip6), respectively. DNA sequences showed that the fragments taken together were the gene encoding PGIP. A2 and A3 contained two exons interrupted by one intron, which has GT-AG sequence. Its DNA and amino acid sequences were highly homologies to those from Prunus Persica; Prunus Salicina; Prunus Americana; Prunus Mume, respectively. A conserved lencinerial fragment exists in the derived protein sequence.http://publications.icdp.ro/publicatii/lucrari%202015/02.Han%20and%20Tian%20China.pdfalmondPGIP DNACloningSequence |
| spellingShingle | Yuhu Han Ge Tian Yanxia Wu Yiwen Ji Qiufen Cao Jian-bao Tian2 CLONING AND SEQUENCING OF PGIP FROM ‘JIN SERIES’ ALMOND (PRUNUS DULCIS) Fruit Growing Research almond PGIP DNA Cloning Sequence |
| title | CLONING AND SEQUENCING OF PGIP FROM ‘JIN SERIES’ ALMOND (PRUNUS DULCIS) |
| title_full | CLONING AND SEQUENCING OF PGIP FROM ‘JIN SERIES’ ALMOND (PRUNUS DULCIS) |
| title_fullStr | CLONING AND SEQUENCING OF PGIP FROM ‘JIN SERIES’ ALMOND (PRUNUS DULCIS) |
| title_full_unstemmed | CLONING AND SEQUENCING OF PGIP FROM ‘JIN SERIES’ ALMOND (PRUNUS DULCIS) |
| title_short | CLONING AND SEQUENCING OF PGIP FROM ‘JIN SERIES’ ALMOND (PRUNUS DULCIS) |
| title_sort | cloning and sequencing of pgip from jin series almond prunus dulcis |
| topic | almond PGIP DNA Cloning Sequence |
| url | http://publications.icdp.ro/publicatii/lucrari%202015/02.Han%20and%20Tian%20China.pdf |
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