<it>In vitro </it>suppression of the <it>MMP-3 </it>gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA
<p>Abstract</p> <p>Background</p> <p>Matrix metalloproteinase (MMPs) synthesized and secreted from connective tissue cells have been thought to participate in degradation of the extracellular matrix. Increased MMPs activities that degrade proteoglycans have been measure...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-12-01
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| Series: | Journal of Orthopaedic Surgery and Research |
| Online Access: | http://www.josr-online.com/content/4/1/45 |
| _version_ | 1798041732439343104 |
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| author | Mekchay Supamit Chomdej Siriwadee Klunklin Kasisin Pothacharoen Peraphan Siengdee Puntita Chaochird Patama Nganvongpanit Korakot Kongtaweelert Prachya |
| author_facet | Mekchay Supamit Chomdej Siriwadee Klunklin Kasisin Pothacharoen Peraphan Siengdee Puntita Chaochird Patama Nganvongpanit Korakot Kongtaweelert Prachya |
| author_sort | Mekchay Supamit |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Matrix metalloproteinase (MMPs) synthesized and secreted from connective tissue cells have been thought to participate in degradation of the extracellular matrix. Increased MMPs activities that degrade proteoglycans have been measured in osteoarthritis cartilage. This study aims to suppress the expression of the <it>MMP-3 </it>gene in <it>in vitro </it>human chondrosarcoma using siRNA.</p> <p>Methods</p> <p>Cells were categorized into four groups: control (G.1); transfection solution treated (G.2); negative control siRNA treated (G.3); and <it>MMP-3 </it>siRNA treated (G.4). All four groups were further subdivided into two groups - treated and non-treated with IL-1β- following culture for 48 and 72 h. We observed the effects of gene suppression according to cell morphology, glycosaminoglycan (GAG) and hyaluronan (HA) production, and gene expression by using real-time polymerase chain reaction (PCR).</p> <p>Results</p> <p>In IL-1β treated cells the apoptosis rate in G.4 was found to be lower than in all other groups, while viability and mitotic rate were higher than in all other groups (<it>p </it>< 0.05). The production of GAG and HA in G.4 was significantly higher than the control group (<it>p </it>< 0.05). <it>MMP-3 </it>gene expression was downregulated significantly (<it>p </it>< 0.05).</p> <p>Conclusion</p> <p><it>MMP-3 </it>specific siRNA can inhibit the expression of <it>MMP-3 </it>in chondrosarcoma. This suggests that <it>MMP-3 </it>siRNA has the potential to be a useful preventive and therapeutic agent for osteoarthritis.</p> |
| first_indexed | 2024-04-11T22:25:37Z |
| format | Article |
| id | doaj.art-815c96b7d32d4038894b3dd29674b1e3 |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-11T22:25:37Z |
| publishDate | 2009-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-815c96b7d32d4038894b3dd29674b1e32022-12-22T03:59:45ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2009-12-01414510.1186/1749-799X-4-45<it>In vitro </it>suppression of the <it>MMP-3 </it>gene in normal and cytokine-treated human chondrosarcoma using small interfering RNAMekchay SupamitChomdej SiriwadeeKlunklin KasisinPothacharoen PeraphanSiengdee PuntitaChaochird PatamaNganvongpanit KorakotKongtaweelert Prachya<p>Abstract</p> <p>Background</p> <p>Matrix metalloproteinase (MMPs) synthesized and secreted from connective tissue cells have been thought to participate in degradation of the extracellular matrix. Increased MMPs activities that degrade proteoglycans have been measured in osteoarthritis cartilage. This study aims to suppress the expression of the <it>MMP-3 </it>gene in <it>in vitro </it>human chondrosarcoma using siRNA.</p> <p>Methods</p> <p>Cells were categorized into four groups: control (G.1); transfection solution treated (G.2); negative control siRNA treated (G.3); and <it>MMP-3 </it>siRNA treated (G.4). All four groups were further subdivided into two groups - treated and non-treated with IL-1β- following culture for 48 and 72 h. We observed the effects of gene suppression according to cell morphology, glycosaminoglycan (GAG) and hyaluronan (HA) production, and gene expression by using real-time polymerase chain reaction (PCR).</p> <p>Results</p> <p>In IL-1β treated cells the apoptosis rate in G.4 was found to be lower than in all other groups, while viability and mitotic rate were higher than in all other groups (<it>p </it>< 0.05). The production of GAG and HA in G.4 was significantly higher than the control group (<it>p </it>< 0.05). <it>MMP-3 </it>gene expression was downregulated significantly (<it>p </it>< 0.05).</p> <p>Conclusion</p> <p><it>MMP-3 </it>specific siRNA can inhibit the expression of <it>MMP-3 </it>in chondrosarcoma. This suggests that <it>MMP-3 </it>siRNA has the potential to be a useful preventive and therapeutic agent for osteoarthritis.</p>http://www.josr-online.com/content/4/1/45 |
| spellingShingle | Mekchay Supamit Chomdej Siriwadee Klunklin Kasisin Pothacharoen Peraphan Siengdee Puntita Chaochird Patama Nganvongpanit Korakot Kongtaweelert Prachya <it>In vitro </it>suppression of the <it>MMP-3 </it>gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA Journal of Orthopaedic Surgery and Research |
| title | <it>In vitro </it>suppression of the <it>MMP-3 </it>gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA |
| title_full | <it>In vitro </it>suppression of the <it>MMP-3 </it>gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA |
| title_fullStr | <it>In vitro </it>suppression of the <it>MMP-3 </it>gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA |
| title_full_unstemmed | <it>In vitro </it>suppression of the <it>MMP-3 </it>gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA |
| title_short | <it>In vitro </it>suppression of the <it>MMP-3 </it>gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA |
| title_sort | it in vitro it suppression of the it mmp 3 it gene in normal and cytokine treated human chondrosarcoma using small interfering rna |
| url | http://www.josr-online.com/content/4/1/45 |
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