A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease.
BACKGROUND:Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counte...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-04-01
|
| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | http://europepmc.org/articles/PMC4851354?pdf=render |
| _version_ | 1818458814761926656 |
|---|---|
| author | Paula Beatriz Santiago Teresa C F Assumpção Carla Nunes de Araújo Izabela Marques Dourado Bastos David Neves Ionizete Garcia da Silva Sébastien Charneau Rayner Myr L Queiroz Tainá Raiol João Victor de Araújo Oliveira Marcelo Valle de Sousa Eric Calvo José M C Ribeiro Jaime M Santana |
| author_facet | Paula Beatriz Santiago Teresa C F Assumpção Carla Nunes de Araújo Izabela Marques Dourado Bastos David Neves Ionizete Garcia da Silva Sébastien Charneau Rayner Myr L Queiroz Tainá Raiol João Victor de Araújo Oliveira Marcelo Valle de Sousa Eric Calvo José M C Ribeiro Jaime M Santana |
| author_sort | Paula Beatriz Santiago |
| collection | DOAJ |
| description | BACKGROUND:Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counteracting host haemostatic, inflammatory, and immune responses. METHODS/PRINCIPAL FINDINGS:Next generation sequencing and mass spectrometry-based protein identification were performed to investigate the content of triatomine R. neglectus saliva. We deposited 4,230 coding DNA sequences (CDS) in GenBank. A set of 636 CDS of proteins of putative secretory nature was extracted from the assembled reads, 73 of them confirmed by proteomic analysis. The sialome of R. neglectus was characterized and serine protease transcripts detected. The presence of ubiquitous protein families was revealed, including lipocalins, serine protease inhibitors, and antigen-5. Metalloproteases, disintegrins, and odorant binding protein families were less abundant. CONCLUSIONS/SIGNIFICANCE:The data presented improve our understanding of hematophagous arthropod sialomes, and aid in understanding hematophagy and the complex interplay among vectors and their vertebrate hosts. |
| first_indexed | 2024-12-14T23:04:27Z |
| format | Article |
| id | doaj.art-815d7ada8d9043179ba08081f0891b9c |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-14T23:04:27Z |
| publishDate | 2016-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-815d7ada8d9043179ba08081f0891b9c2022-12-21T22:44:22ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352016-04-01104e000458110.1371/journal.pntd.0004581A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease.Paula Beatriz SantiagoTeresa C F AssumpçãoCarla Nunes de AraújoIzabela Marques Dourado BastosDavid NevesIonizete Garcia da SilvaSébastien CharneauRayner Myr L QueirozTainá RaiolJoão Victor de Araújo OliveiraMarcelo Valle de SousaEric CalvoJosé M C RibeiroJaime M SantanaBACKGROUND:Triatomines are hematophagous insects that act as vectors of Chagas disease. Rhodnius neglectus is one of these kissing bugs found, contributing to the transmission of this American trypanosomiasis. The saliva of hematophagous arthropods contains bioactive molecules responsible for counteracting host haemostatic, inflammatory, and immune responses. METHODS/PRINCIPAL FINDINGS:Next generation sequencing and mass spectrometry-based protein identification were performed to investigate the content of triatomine R. neglectus saliva. We deposited 4,230 coding DNA sequences (CDS) in GenBank. A set of 636 CDS of proteins of putative secretory nature was extracted from the assembled reads, 73 of them confirmed by proteomic analysis. The sialome of R. neglectus was characterized and serine protease transcripts detected. The presence of ubiquitous protein families was revealed, including lipocalins, serine protease inhibitors, and antigen-5. Metalloproteases, disintegrins, and odorant binding protein families were less abundant. CONCLUSIONS/SIGNIFICANCE:The data presented improve our understanding of hematophagous arthropod sialomes, and aid in understanding hematophagy and the complex interplay among vectors and their vertebrate hosts.http://europepmc.org/articles/PMC4851354?pdf=render |
| spellingShingle | Paula Beatriz Santiago Teresa C F Assumpção Carla Nunes de Araújo Izabela Marques Dourado Bastos David Neves Ionizete Garcia da Silva Sébastien Charneau Rayner Myr L Queiroz Tainá Raiol João Victor de Araújo Oliveira Marcelo Valle de Sousa Eric Calvo José M C Ribeiro Jaime M Santana A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. PLoS Neglected Tropical Diseases |
| title | A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. |
| title_full | A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. |
| title_fullStr | A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. |
| title_full_unstemmed | A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. |
| title_short | A Deep Insight into the Sialome of Rhodnius neglectus, a Vector of Chagas Disease. |
| title_sort | deep insight into the sialome of rhodnius neglectus a vector of chagas disease |
| url | http://europepmc.org/articles/PMC4851354?pdf=render |
| work_keys_str_mv | AT paulabeatrizsantiago adeepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT teresacfassumpcao adeepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT carlanunesdearaujo adeepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT izabelamarquesdouradobastos adeepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT davidneves adeepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT ionizetegarciadasilva adeepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT sebastiencharneau adeepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT raynermyrlqueiroz adeepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT tainaraiol adeepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT joaovictordearaujooliveira adeepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT marcelovalledesousa adeepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT ericcalvo adeepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT josemcribeiro adeepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT jaimemsantana adeepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT paulabeatrizsantiago deepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT teresacfassumpcao deepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT carlanunesdearaujo deepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT izabelamarquesdouradobastos deepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT davidneves deepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT ionizetegarciadasilva deepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT sebastiencharneau deepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT raynermyrlqueiroz deepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT tainaraiol deepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT joaovictordearaujooliveira deepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT marcelovalledesousa deepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT ericcalvo deepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT josemcribeiro deepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease AT jaimemsantana deepinsightintothesialomeofrhodniusneglectusavectorofchagasdisease |