Genome-Wide Analysis of the <i>Liriodendron chinense Hsf</i> Gene Family under Abiotic Stress and Characterization of the <i>LcHsfA2a</i> Gene
Heat shock factors (Hsfs) play a crucial role in plant defense processes. However, the distribution and functional characteristics of Hsf genes in the relict plant <i>Liriodendron chinense</i> are still unclear. In this study, a total of 19 <i>LcHsfs</i> were identified and d...
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| author | Yun Yang Jianchao Yin Liming Zhu Lin Xu Weihuang Wu Ye Lu Jinhui Chen Jisen Shi Zhaodong Hao |
| author_facet | Yun Yang Jianchao Yin Liming Zhu Lin Xu Weihuang Wu Ye Lu Jinhui Chen Jisen Shi Zhaodong Hao |
| author_sort | Yun Yang |
| collection | DOAJ |
| description | Heat shock factors (Hsfs) play a crucial role in plant defense processes. However, the distribution and functional characteristics of Hsf genes in the relict plant <i>Liriodendron chinense</i> are still unclear. In this study, a total of 19 <i>LcHsfs</i> were identified and divided into three separate subgroups, comprising 10 <i>LcHsfA</i>, 7 <i>LcHsfB</i>, and 2 <i>LcHsfC</i> genes, respectively, based on their phylogenetic tree and the presence/absence of conserved protein domains. Whole-genome duplication and segmental duplication led to an expansion of the <i>LhHsf</i> gene family. The promoters of <i>LcHsf</i> genes are enriched for different types of <i>cis</i>-acting elements, including hormone responsive and abiotic-stress-responsive elements. The expression of <i>LcHsfA3</i>, <i>LcHsfA4b</i>, <i>LcHsfA5</i>, <i>LcHsfB1b</i>, and <i>LcHsfB2b</i> increased significantly as a result of both cold and drought treatments. <i>LcHsfA2a</i>, <i>LcHsfA2b</i>, and <i>LcHsfA7</i> act as important genes whose expression levels correlate strongly with the expression of the <i>LcHsp70</i>, <i>LcHsp110</i>, and <i>LcAPX</i> genes under heat stress. In addition, we found that transiently transformed <i>35S:LcHsfA2a</i> seedlings showed significantly lower levels of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) after heat stress and showed a stronger thermotolerance. This study sheds light on the possible functions of <i>LcHsf</i> genes under abiotic stress and identifies potentially useful genes to target for molecular breeding, in order to develop more stress-resistant varieties. |
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| language | English |
| last_indexed | 2024-04-25T00:29:04Z |
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| spelling | doaj.art-816854ac36df46e497c49d5e89ed67742024-03-12T16:46:08ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01255273310.3390/ijms25052733Genome-Wide Analysis of the <i>Liriodendron chinense Hsf</i> Gene Family under Abiotic Stress and Characterization of the <i>LcHsfA2a</i> GeneYun Yang0Jianchao Yin1Liming Zhu2Lin Xu3Weihuang Wu4Ye Lu5Jinhui Chen6Jisen Shi7Zhaodong Hao8State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaState Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaHeat shock factors (Hsfs) play a crucial role in plant defense processes. However, the distribution and functional characteristics of Hsf genes in the relict plant <i>Liriodendron chinense</i> are still unclear. In this study, a total of 19 <i>LcHsfs</i> were identified and divided into three separate subgroups, comprising 10 <i>LcHsfA</i>, 7 <i>LcHsfB</i>, and 2 <i>LcHsfC</i> genes, respectively, based on their phylogenetic tree and the presence/absence of conserved protein domains. Whole-genome duplication and segmental duplication led to an expansion of the <i>LhHsf</i> gene family. The promoters of <i>LcHsf</i> genes are enriched for different types of <i>cis</i>-acting elements, including hormone responsive and abiotic-stress-responsive elements. The expression of <i>LcHsfA3</i>, <i>LcHsfA4b</i>, <i>LcHsfA5</i>, <i>LcHsfB1b</i>, and <i>LcHsfB2b</i> increased significantly as a result of both cold and drought treatments. <i>LcHsfA2a</i>, <i>LcHsfA2b</i>, and <i>LcHsfA7</i> act as important genes whose expression levels correlate strongly with the expression of the <i>LcHsp70</i>, <i>LcHsp110</i>, and <i>LcAPX</i> genes under heat stress. In addition, we found that transiently transformed <i>35S:LcHsfA2a</i> seedlings showed significantly lower levels of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) after heat stress and showed a stronger thermotolerance. This study sheds light on the possible functions of <i>LcHsf</i> genes under abiotic stress and identifies potentially useful genes to target for molecular breeding, in order to develop more stress-resistant varieties.https://www.mdpi.com/1422-0067/25/5/2733<i>Liriodendron chinense</i><i>Hsf</i> gene family<i>LcHsfA2</i>abiotic stress |
| spellingShingle | Yun Yang Jianchao Yin Liming Zhu Lin Xu Weihuang Wu Ye Lu Jinhui Chen Jisen Shi Zhaodong Hao Genome-Wide Analysis of the <i>Liriodendron chinense Hsf</i> Gene Family under Abiotic Stress and Characterization of the <i>LcHsfA2a</i> Gene International Journal of Molecular Sciences <i>Liriodendron chinense</i> <i>Hsf</i> gene family <i>LcHsfA2</i> abiotic stress |
| title | Genome-Wide Analysis of the <i>Liriodendron chinense Hsf</i> Gene Family under Abiotic Stress and Characterization of the <i>LcHsfA2a</i> Gene |
| title_full | Genome-Wide Analysis of the <i>Liriodendron chinense Hsf</i> Gene Family under Abiotic Stress and Characterization of the <i>LcHsfA2a</i> Gene |
| title_fullStr | Genome-Wide Analysis of the <i>Liriodendron chinense Hsf</i> Gene Family under Abiotic Stress and Characterization of the <i>LcHsfA2a</i> Gene |
| title_full_unstemmed | Genome-Wide Analysis of the <i>Liriodendron chinense Hsf</i> Gene Family under Abiotic Stress and Characterization of the <i>LcHsfA2a</i> Gene |
| title_short | Genome-Wide Analysis of the <i>Liriodendron chinense Hsf</i> Gene Family under Abiotic Stress and Characterization of the <i>LcHsfA2a</i> Gene |
| title_sort | genome wide analysis of the i liriodendron chinense hsf i gene family under abiotic stress and characterization of the i lchsfa2a i gene |
| topic | <i>Liriodendron chinense</i> <i>Hsf</i> gene family <i>LcHsfA2</i> abiotic stress |
| url | https://www.mdpi.com/1422-0067/25/5/2733 |
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