Immunohistochemical Analysis of P53 and PRB in Esophageal Squamous Cell Carcinoma
Background: Esophageal squamous cell carcinoma (ESCC) is one of the fatal cancers with a high incidence rate in Asia. Many genes including p53 and pRb play an important role in its carcinogenesis. Expressions of p53 and pRb proteins have been associated with prognosis of ESCC. Objective: The st...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2014-05-01
|
| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/4309/7428_E(Ra)_F(P)_PF1(AK)_PFA(AK)_PF2(PAG)_PF2(PN).pdf |
| Summary: | Background: Esophageal squamous cell carcinoma (ESCC)
is one of the fatal cancers with a high incidence rate in Asia.
Many genes including p53 and pRb play an important role in its
carcinogenesis. Expressions of p53 and pRb proteins have been
associated with prognosis of ESCC.
Objective: The study was undertaken to analyze the expressions
of p53 and pRb with the various clinicopathological characteristics
including stage and grade of ESCC.
Materials and Methods: We examined 30 biopsy samples
of ESCC for p53 and pRb protein expressions using
immunohistochemistry. Immunointensity was classified as
no immunostaining (-), weakly immunostaining (+), weak
immunostaining (++) and strongly positive immunostaining
(+++).
Results: Significant association was seen between positive
p53 expression and tumor size (p=0.042), invasion to adventitia
(p=0.009) and lymph node metastasis (p=0.014), and negative
pRb expression was associated with invasion to the adventitia
(p=0.015) and lymph node metastasis (p=0.003) There was
significant association of p53+/ pRb- (n=11) with tumor invasion
to adventitia (p=0.001), lymph node metastasis (p=0.0007) and
TNM staging (p=0.042).
Conclusion: The study shows that p53 and pRb proteins,
individually as well as synergistically, may serve as putative
prognostic markers in ESCC. |
|---|---|
| ISSN: | 2249-782X 0973-709X |