Optimization of Dynamic High Pressure Microfluidization-Assisted Extraction of Burdock Root Polysaccharide and Its in Vitro Antioxidant Activity
In this paper, the extraction process of burdock root polysaccharide was optimized using dynamic high pressure microfluidization (DHPM) combined with water extraction, and its infrared spectral structure and monosaccharide composition were analyzed to evaluate its scavenging activities against DPPH·...
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The editorial department of Science and Technology of Food Industry
2023-06-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022070133 |
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| author | Chenchen ZHANG Jiangfeng SONG Yuanfa LIU Ningning GENG Kaiyang MA Lin WANG Ying LI |
| author_facet | Chenchen ZHANG Jiangfeng SONG Yuanfa LIU Ningning GENG Kaiyang MA Lin WANG Ying LI |
| author_sort | Chenchen ZHANG |
| collection | DOAJ |
| description | In this paper, the extraction process of burdock root polysaccharide was optimized using dynamic high pressure microfluidization (DHPM) combined with water extraction, and its infrared spectral structure and monosaccharide composition were analyzed to evaluate its scavenging activities against DPPH·, ·OH and ABTS＋· radicals. The results showed that the factors affecting the yield of burdock root polysaccharide were DHPM pressure>extraction temperature>liquid to solid ratio>extraction time> number of treatments in descending order. The optimal extraction conditions were determined by response surface methodology: DHPM pressure of 148.0 MPa, liquid-solid ratio of 25:1 mL/g, two times of treatment, extraction temperature of 63 ℃ and extraction time of 1 h. Under these conditions, the predicted yield of burdock root polysaccharide reached 29.6%, and the relative error between the actual yield of 29.7% and predicted values was only 0.3%. The polysaccharides were mainly composed of fructose, glucose, arabinose, galactose and glucosamine hydrochloride, with molar ratios of 0.844:0.122:0.024:0.008:0.002. The polysaccharides of burdock root had different effects on DPPH·, ABTS+· and ·OH radicals. The IC50 values were 0.30, 0.36 and 3.93 mg/mL, respectively. The study would provide a reference for the green and efficient extraction of burdock root polysaccharide. |
| first_indexed | 2024-03-12T13:34:27Z |
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| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-12T13:34:27Z |
| publishDate | 2023-06-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-8174a3a176d84539b4f26f605e381c692023-08-24T06:00:59ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-06-01441226026710.13386/j.issn1002-0306.20220701332022070133-12Optimization of Dynamic High Pressure Microfluidization-Assisted Extraction of Burdock Root Polysaccharide and Its in Vitro Antioxidant ActivityChenchen ZHANG0Jiangfeng SONG1Yuanfa LIU2Ningning GENG3Kaiyang MA4Lin WANG5Ying LI6School of Food and Bioengineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Bioengineering, Jiangsu University, Zhenjiang 212013, ChinaFuture Food Bai Ma Research Institute, Nanjing 211225, ChinaSchool of Food and Bioengineering, Jiangsu University, Zhenjiang 212013, ChinaInstitute of Agro-Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaSchool of Food and Bioengineering, Jiangsu University, Zhenjiang 212013, ChinaInstitute of Agro-Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaIn this paper, the extraction process of burdock root polysaccharide was optimized using dynamic high pressure microfluidization (DHPM) combined with water extraction, and its infrared spectral structure and monosaccharide composition were analyzed to evaluate its scavenging activities against DPPH·, ·OH and ABTS＋· radicals. The results showed that the factors affecting the yield of burdock root polysaccharide were DHPM pressure>extraction temperature>liquid to solid ratio>extraction time> number of treatments in descending order. The optimal extraction conditions were determined by response surface methodology: DHPM pressure of 148.0 MPa, liquid-solid ratio of 25:1 mL/g, two times of treatment, extraction temperature of 63 ℃ and extraction time of 1 h. Under these conditions, the predicted yield of burdock root polysaccharide reached 29.6%, and the relative error between the actual yield of 29.7% and predicted values was only 0.3%. The polysaccharides were mainly composed of fructose, glucose, arabinose, galactose and glucosamine hydrochloride, with molar ratios of 0.844:0.122:0.024:0.008:0.002. The polysaccharides of burdock root had different effects on DPPH·, ABTS+· and ·OH radicals. The IC50 values were 0.30, 0.36 and 3.93 mg/mL, respectively. The study would provide a reference for the green and efficient extraction of burdock root polysaccharide.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022070133burdock rootpolysaccharide extractiondynamic high pressure microfluidizationmonosaccharide compositionantioxidant activity |
| spellingShingle | Chenchen ZHANG Jiangfeng SONG Yuanfa LIU Ningning GENG Kaiyang MA Lin WANG Ying LI Optimization of Dynamic High Pressure Microfluidization-Assisted Extraction of Burdock Root Polysaccharide and Its in Vitro Antioxidant Activity Shipin gongye ke-ji burdock root polysaccharide extraction dynamic high pressure microfluidization monosaccharide composition antioxidant activity |
| title | Optimization of Dynamic High Pressure Microfluidization-Assisted Extraction of Burdock Root Polysaccharide and Its in Vitro Antioxidant Activity |
| title_full | Optimization of Dynamic High Pressure Microfluidization-Assisted Extraction of Burdock Root Polysaccharide and Its in Vitro Antioxidant Activity |
| title_fullStr | Optimization of Dynamic High Pressure Microfluidization-Assisted Extraction of Burdock Root Polysaccharide and Its in Vitro Antioxidant Activity |
| title_full_unstemmed | Optimization of Dynamic High Pressure Microfluidization-Assisted Extraction of Burdock Root Polysaccharide and Its in Vitro Antioxidant Activity |
| title_short | Optimization of Dynamic High Pressure Microfluidization-Assisted Extraction of Burdock Root Polysaccharide and Its in Vitro Antioxidant Activity |
| title_sort | optimization of dynamic high pressure microfluidization assisted extraction of burdock root polysaccharide and its in vitro antioxidant activity |
| topic | burdock root polysaccharide extraction dynamic high pressure microfluidization monosaccharide composition antioxidant activity |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022070133 |
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