Gut microbiota profiling variated during colorectal cancer development in mouse
Abstract Background The imbalance of intestinal flora may promote the occurrence and development of colorectal cancer, changes of the intestinal flora during the development of colorectal cancer and the mechanism that promotes the colorectal cancer were discovered in this study. Deep sequencing of t...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-022-09008-3 |
| _version_ | 1797977599977193472 |
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| author | Jingjing Liu Wei Dong Jian Zhao Jing Wu Jinqiang Xia Shaofei Xie Xiaofeng Song |
| author_facet | Jingjing Liu Wei Dong Jian Zhao Jing Wu Jinqiang Xia Shaofei Xie Xiaofeng Song |
| author_sort | Jingjing Liu |
| collection | DOAJ |
| description | Abstract Background The imbalance of intestinal flora may promote the occurrence and development of colorectal cancer, changes of the intestinal flora during the development of colorectal cancer and the mechanism that promotes the colorectal cancer were discovered in this study. Deep sequencing of the microbial 16 s ribosomal RNA gene was used to investigate alterations in feces samples of mice at the early inflammation stage and fully developed stage of colorectal cancer. Results According to PCoA analysis and ANOSIM test, we found the intestinal flora had significantly changed in mice with colorectal inflammation or colorectal cancer compared with healthy mice (p < 0.05). Using correlation analysis, we found that Muribaculaceae and Bacteroidaceae had strong excluding interactions. The functional changes of the gut microbiota include the up-regulation of the cancers pathway and the down-regulation of the replication and repair pathways. Conclusion Our study found the intestinal flora of mice suffering from colorectal inflammation and colorectal cancer has changed significantly, especially the decrease of Muribaculaceae and the increase of Bacteroidaceae. We suppose that these two floras may play an important role in development of colorectal cancer. |
| first_indexed | 2024-04-11T05:09:44Z |
| format | Article |
| id | doaj.art-8174a3d6c1dc484d814428857b807de8 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-11T05:09:44Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-8174a3d6c1dc484d814428857b807de82022-12-25T12:06:19ZengBMCBMC Genomics1471-21642022-12-0123S411310.1186/s12864-022-09008-3Gut microbiota profiling variated during colorectal cancer development in mouseJingjing Liu0Wei Dong1Jian Zhao2Jing Wu3Jinqiang Xia4Shaofei Xie5Xiaofeng Song6Department of Biomedical Engineering, Nanjing University of Aeronautics and AstronauticsDepartment of Biomedical Engineering, Nanjing University of Aeronautics and AstronauticsDepartment of Biomedical Engineering, Nanjing University of Aeronautics and AstronauticsSchool of Biomedical Engineering and Informatics, Nanjing Medical UniversityThe State Key Laboratory of Translational Medicine and Innovative Drug Development, Jiangsu Simcere pharmaceutical Co., LtdThe State Key Laboratory of Translational Medicine and Innovative Drug Development, Jiangsu Simcere pharmaceutical Co., LtdDepartment of Biomedical Engineering, Nanjing University of Aeronautics and AstronauticsAbstract Background The imbalance of intestinal flora may promote the occurrence and development of colorectal cancer, changes of the intestinal flora during the development of colorectal cancer and the mechanism that promotes the colorectal cancer were discovered in this study. Deep sequencing of the microbial 16 s ribosomal RNA gene was used to investigate alterations in feces samples of mice at the early inflammation stage and fully developed stage of colorectal cancer. Results According to PCoA analysis and ANOSIM test, we found the intestinal flora had significantly changed in mice with colorectal inflammation or colorectal cancer compared with healthy mice (p < 0.05). Using correlation analysis, we found that Muribaculaceae and Bacteroidaceae had strong excluding interactions. The functional changes of the gut microbiota include the up-regulation of the cancers pathway and the down-regulation of the replication and repair pathways. Conclusion Our study found the intestinal flora of mice suffering from colorectal inflammation and colorectal cancer has changed significantly, especially the decrease of Muribaculaceae and the increase of Bacteroidaceae. We suppose that these two floras may play an important role in development of colorectal cancer.https://doi.org/10.1186/s12864-022-09008-3Colorectal inflammationColorectal cancerGut microbiota16S rRNA |
| spellingShingle | Jingjing Liu Wei Dong Jian Zhao Jing Wu Jinqiang Xia Shaofei Xie Xiaofeng Song Gut microbiota profiling variated during colorectal cancer development in mouse BMC Genomics Colorectal inflammation Colorectal cancer Gut microbiota 16S rRNA |
| title | Gut microbiota profiling variated during colorectal cancer development in mouse |
| title_full | Gut microbiota profiling variated during colorectal cancer development in mouse |
| title_fullStr | Gut microbiota profiling variated during colorectal cancer development in mouse |
| title_full_unstemmed | Gut microbiota profiling variated during colorectal cancer development in mouse |
| title_short | Gut microbiota profiling variated during colorectal cancer development in mouse |
| title_sort | gut microbiota profiling variated during colorectal cancer development in mouse |
| topic | Colorectal inflammation Colorectal cancer Gut microbiota 16S rRNA |
| url | https://doi.org/10.1186/s12864-022-09008-3 |
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