Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models
Abstract Background Justicia secunda Vahl. is a medicinal plant used in ethnomedical practice as therapy to manage inflammation. Therefore, this study was designed to evaluate the anti-inflammatory activity of methanol extract of J. secunda leaves (MEJSL) using in vitro and in vivo inflammation mode...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2019-12-01
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| Series: | Clinical Phytoscience |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40816-019-0137-8 |
| _version_ | 1819295546783825920 |
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| author | Godswill Nduka Anyasor Azeezat Adenike Okanlawon Babafemi Ogunbiyi |
| author_facet | Godswill Nduka Anyasor Azeezat Adenike Okanlawon Babafemi Ogunbiyi |
| author_sort | Godswill Nduka Anyasor |
| collection | DOAJ |
| description | Abstract Background Justicia secunda Vahl. is a medicinal plant used in ethnomedical practice as therapy to manage inflammation. Therefore, this study was designed to evaluate the anti-inflammatory activity of methanol extract of J. secunda leaves (MEJSL) using in vitro and in vivo inflammation models. Methods Seventy-percent MEJSL was prepared following standard procedure. In vitro anti-inflammatory assays were performed using heat-induced bovine serum albumin (BSA) denaturation and erythrocyte membrane stabilization assays. Carrageenan and formaldehyde induced inflammation in rat models were used to evaluate the anti-inflammatory activity of MEJSL in vivo. Diclofenac sodium was used as a reference drug. In addition, liver and kidney function assays and hematological analysis were carried out. Results Data revealed that varying concentrations of MEJSL significantly (P < 0.05) inhibited heat-induced BSA denaturation and stabilized erythrocyte membrane against hypotonicity-induced hemolysis when compared with diclofenac sodium in a concentration-dependent manner. In vivo study showed that 10 mg/kg body weight (b.w.) diclofenac sodium, 100 and 300 mg/kg b.w. MEJSL suppressed carrageenan-induced paw edema at the sixth hour by 71.14%, 83.08%, and 89.05%, respectively. Furthermore, 10 mg/kg b.w. diclofenac sodium, 100 and 300 mg/kg b.w. MEJSL inhibited formaldehyde-induced paw edema by 72.53%, 74.73%, and 76.48%, respectively. Animals treated with varying doses of MEJSL had reduced plasma aspartate aminotransferase and alanine aminotransferase activities; urea and creatinine concentrations; and modulated hematological parameters when compared with the untreated control group. Conclusions Findings from this study showed that MEJSL exhibited substantial anti-inflammatory actions in the in vitro and in vivo models. It also indicated that MEJSL anti-inflammatory mechanisms of action could be through interference with phase 2 inflammatory stressors, upregulation of cytoprotective genes, stabilization of inflammatory cell membranes and immunomodulatory activity. |
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| format | Article |
| id | doaj.art-8176f8638b91464981ce6f3988b77de0 |
| institution | Directory Open Access Journal |
| issn | 2199-1197 |
| language | English |
| last_indexed | 2024-12-24T04:43:56Z |
| publishDate | 2019-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Clinical Phytoscience |
| spelling | doaj.art-8176f8638b91464981ce6f3988b77de02022-12-21T17:14:44ZengSpringerOpenClinical Phytoscience2199-11972019-12-015111310.1186/s40816-019-0137-8Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation modelsGodswill Nduka Anyasor0Azeezat Adenike Okanlawon1Babafemi Ogunbiyi2Department of Biochemistry, Benjamin S. Carson (Snr.) School of Medicine, Babcock UniversityDepartment of Biochemistry, Benjamin S. Carson (Snr.) School of Medicine, Babcock UniversityDepartment of Biochemistry, Benjamin S. Carson (Snr.) School of Medicine, Babcock UniversityAbstract Background Justicia secunda Vahl. is a medicinal plant used in ethnomedical practice as therapy to manage inflammation. Therefore, this study was designed to evaluate the anti-inflammatory activity of methanol extract of J. secunda leaves (MEJSL) using in vitro and in vivo inflammation models. Methods Seventy-percent MEJSL was prepared following standard procedure. In vitro anti-inflammatory assays were performed using heat-induced bovine serum albumin (BSA) denaturation and erythrocyte membrane stabilization assays. Carrageenan and formaldehyde induced inflammation in rat models were used to evaluate the anti-inflammatory activity of MEJSL in vivo. Diclofenac sodium was used as a reference drug. In addition, liver and kidney function assays and hematological analysis were carried out. Results Data revealed that varying concentrations of MEJSL significantly (P < 0.05) inhibited heat-induced BSA denaturation and stabilized erythrocyte membrane against hypotonicity-induced hemolysis when compared with diclofenac sodium in a concentration-dependent manner. In vivo study showed that 10 mg/kg body weight (b.w.) diclofenac sodium, 100 and 300 mg/kg b.w. MEJSL suppressed carrageenan-induced paw edema at the sixth hour by 71.14%, 83.08%, and 89.05%, respectively. Furthermore, 10 mg/kg b.w. diclofenac sodium, 100 and 300 mg/kg b.w. MEJSL inhibited formaldehyde-induced paw edema by 72.53%, 74.73%, and 76.48%, respectively. Animals treated with varying doses of MEJSL had reduced plasma aspartate aminotransferase and alanine aminotransferase activities; urea and creatinine concentrations; and modulated hematological parameters when compared with the untreated control group. Conclusions Findings from this study showed that MEJSL exhibited substantial anti-inflammatory actions in the in vitro and in vivo models. It also indicated that MEJSL anti-inflammatory mechanisms of action could be through interference with phase 2 inflammatory stressors, upregulation of cytoprotective genes, stabilization of inflammatory cell membranes and immunomodulatory activity.https://doi.org/10.1186/s40816-019-0137-8Justicia secundaAnti-inflammationCarrageenanFormaldehydeHematologyAnd immunology |
| spellingShingle | Godswill Nduka Anyasor Azeezat Adenike Okanlawon Babafemi Ogunbiyi Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models Clinical Phytoscience Justicia secunda Anti-inflammation Carrageenan Formaldehyde Hematology And immunology |
| title | Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models |
| title_full | Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models |
| title_fullStr | Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models |
| title_full_unstemmed | Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models |
| title_short | Evaluation of anti-inflammatory activity of Justicia secunda Vahl leaf extract using in vitro and in vivo inflammation models |
| title_sort | evaluation of anti inflammatory activity of justicia secunda vahl leaf extract using in vitro and in vivo inflammation models |
| topic | Justicia secunda Anti-inflammation Carrageenan Formaldehyde Hematology And immunology |
| url | https://doi.org/10.1186/s40816-019-0137-8 |
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