Elimination of Curtobacterium sp. strain A7_M15, a contaminant in Prunus rootstock tissue culture production, using reduced graphene oxide–silver–copper and silver–selenium nanocomposites
Abstract Background Bacterial contamination poses a high risk to the successful establishment and maintenance of plant tissue cultures. The aim of this study was to identify the isolates representing the frequent bacterial contaminants of Prunus rootstock tissue cultures and to determine the most ef...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2024-01-01
|
| Series: | Chemical and Biological Technologies in Agriculture |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40538-024-00536-6 |
| _version_ | 1797276687327559680 |
|---|---|
| author | Dorota Tekielska Jakub Pečenka Eliška Hakalová Jana Čechová Zuzana Bytešníková Lukáš Richtera Tomáš Kiss Aleš Eichmeier Miroslav Baránek |
| author_facet | Dorota Tekielska Jakub Pečenka Eliška Hakalová Jana Čechová Zuzana Bytešníková Lukáš Richtera Tomáš Kiss Aleš Eichmeier Miroslav Baránek |
| author_sort | Dorota Tekielska |
| collection | DOAJ |
| description | Abstract Background Bacterial contamination poses a high risk to the successful establishment and maintenance of plant tissue cultures. The aim of this study was to identify the isolates representing the frequent bacterial contaminants of Prunus rootstock tissue cultures and to determine the most effective concentration of nanomaterials for Curtobacterium sp. strain A7_M15 elimination without a negative impact on explants. Results Six Curtobacterium sp. strains were isolated and identified, and the whole-genome sequence was obtained for strain A7_M15. Two nanocomposites, reduced graphene oxide–copper–silver and silver–selenium, with the highest bactericidal activity were selected for elimination of Curtobacterium sp. contamination in Gisela 5 rootstock tissue cultures. Both nanocomposites showed 100% inhibition of bacterial plaque formation on culture medium at concentrations of 100, 200 and 400 mg L-1 Ag (2 ×–8 × MBC). The quantity of Curtobacterium sp. on culture medium assessed using cfu enumeration was reduced by 92% and 74% in comparison to the positive control after treatment with reduced graphene oxide–silver–copper and silver–selenium at a concentration of 200 mg L-1 Ag, respectively. None of the tested concentrations resulted in a decrease in Curtobacterium sp. quantity in explants. Curtobacterium sp. was detected in donor Gisela 5 plants, indicating an endophytic character of this bacterium. The dry weight of explants was not negatively affected by the application of nanocomposites regardless of concentration, and no detrimental effect of either nanocomposite at 100 or 200 mg L-1 Ag on the surface covered by plants was observed. Conclusions Reduced graphene oxide–silver–copper and silver–selenium nanocomposites at 200 mg L-1 Ag effectively limited the Curtobacterium sp. presence in micropropagated Prunus rootstock without causing phytotoxicity; therefore, those treatments could be offered as prevention with a high activity against bacterial contamination in plant tissue cultures. Graphical Abstract |
| first_indexed | 2024-03-07T15:31:48Z |
| format | Article |
| id | doaj.art-8177237e95d04051bd5c882b8d07077b |
| institution | Directory Open Access Journal |
| issn | 2196-5641 |
| language | English |
| last_indexed | 2024-03-07T15:31:48Z |
| publishDate | 2024-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Chemical and Biological Technologies in Agriculture |
| spelling | doaj.art-8177237e95d04051bd5c882b8d07077b2024-03-05T16:22:13ZengSpringerOpenChemical and Biological Technologies in Agriculture2196-56412024-01-0111112110.1186/s40538-024-00536-6Elimination of Curtobacterium sp. strain A7_M15, a contaminant in Prunus rootstock tissue culture production, using reduced graphene oxide–silver–copper and silver–selenium nanocompositesDorota Tekielska0Jakub Pečenka1Eliška Hakalová2Jana Čechová3Zuzana Bytešníková4Lukáš Richtera5Tomáš Kiss6Aleš Eichmeier7Miroslav Baránek8Mendeleum—Institute of Genetics, Faculty of Horticulture, Mendel University in BrnoMendeleum—Institute of Genetics, Faculty of Horticulture, Mendel University in BrnoMendeleum—Institute of Genetics, Faculty of Horticulture, Mendel University in BrnoMendeleum—Institute of Genetics, Faculty of Horticulture, Mendel University in BrnoDepartment of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in BrnoDepartment of Chemistry and Biochemistry, Faculty of AgriSciences, Mendel University in BrnoDepartment of Fruit Growing, Faculty of Horticulture, Mendel University in BrnoMendeleum—Institute of Genetics, Faculty of Horticulture, Mendel University in BrnoMendeleum—Institute of Genetics, Faculty of Horticulture, Mendel University in BrnoAbstract Background Bacterial contamination poses a high risk to the successful establishment and maintenance of plant tissue cultures. The aim of this study was to identify the isolates representing the frequent bacterial contaminants of Prunus rootstock tissue cultures and to determine the most effective concentration of nanomaterials for Curtobacterium sp. strain A7_M15 elimination without a negative impact on explants. Results Six Curtobacterium sp. strains were isolated and identified, and the whole-genome sequence was obtained for strain A7_M15. Two nanocomposites, reduced graphene oxide–copper–silver and silver–selenium, with the highest bactericidal activity were selected for elimination of Curtobacterium sp. contamination in Gisela 5 rootstock tissue cultures. Both nanocomposites showed 100% inhibition of bacterial plaque formation on culture medium at concentrations of 100, 200 and 400 mg L-1 Ag (2 ×–8 × MBC). The quantity of Curtobacterium sp. on culture medium assessed using cfu enumeration was reduced by 92% and 74% in comparison to the positive control after treatment with reduced graphene oxide–silver–copper and silver–selenium at a concentration of 200 mg L-1 Ag, respectively. None of the tested concentrations resulted in a decrease in Curtobacterium sp. quantity in explants. Curtobacterium sp. was detected in donor Gisela 5 plants, indicating an endophytic character of this bacterium. The dry weight of explants was not negatively affected by the application of nanocomposites regardless of concentration, and no detrimental effect of either nanocomposite at 100 or 200 mg L-1 Ag on the surface covered by plants was observed. Conclusions Reduced graphene oxide–silver–copper and silver–selenium nanocomposites at 200 mg L-1 Ag effectively limited the Curtobacterium sp. presence in micropropagated Prunus rootstock without causing phytotoxicity; therefore, those treatments could be offered as prevention with a high activity against bacterial contamination in plant tissue cultures. Graphical Abstracthttps://doi.org/10.1186/s40538-024-00536-6NanomaterialNanocompositeReduced graphene oxideSilverCurtobacteriumPrunus |
| spellingShingle | Dorota Tekielska Jakub Pečenka Eliška Hakalová Jana Čechová Zuzana Bytešníková Lukáš Richtera Tomáš Kiss Aleš Eichmeier Miroslav Baránek Elimination of Curtobacterium sp. strain A7_M15, a contaminant in Prunus rootstock tissue culture production, using reduced graphene oxide–silver–copper and silver–selenium nanocomposites Chemical and Biological Technologies in Agriculture Nanomaterial Nanocomposite Reduced graphene oxide Silver Curtobacterium Prunus |
| title | Elimination of Curtobacterium sp. strain A7_M15, a contaminant in Prunus rootstock tissue culture production, using reduced graphene oxide–silver–copper and silver–selenium nanocomposites |
| title_full | Elimination of Curtobacterium sp. strain A7_M15, a contaminant in Prunus rootstock tissue culture production, using reduced graphene oxide–silver–copper and silver–selenium nanocomposites |
| title_fullStr | Elimination of Curtobacterium sp. strain A7_M15, a contaminant in Prunus rootstock tissue culture production, using reduced graphene oxide–silver–copper and silver–selenium nanocomposites |
| title_full_unstemmed | Elimination of Curtobacterium sp. strain A7_M15, a contaminant in Prunus rootstock tissue culture production, using reduced graphene oxide–silver–copper and silver–selenium nanocomposites |
| title_short | Elimination of Curtobacterium sp. strain A7_M15, a contaminant in Prunus rootstock tissue culture production, using reduced graphene oxide–silver–copper and silver–selenium nanocomposites |
| title_sort | elimination of curtobacterium sp strain a7 m15 a contaminant in prunus rootstock tissue culture production using reduced graphene oxide silver copper and silver selenium nanocomposites |
| topic | Nanomaterial Nanocomposite Reduced graphene oxide Silver Curtobacterium Prunus |
| url | https://doi.org/10.1186/s40538-024-00536-6 |
| work_keys_str_mv | AT dorotatekielska eliminationofcurtobacteriumspstraina7m15acontaminantinprunusrootstocktissuecultureproductionusingreducedgrapheneoxidesilvercopperandsilverseleniumnanocomposites AT jakubpecenka eliminationofcurtobacteriumspstraina7m15acontaminantinprunusrootstocktissuecultureproductionusingreducedgrapheneoxidesilvercopperandsilverseleniumnanocomposites AT eliskahakalova eliminationofcurtobacteriumspstraina7m15acontaminantinprunusrootstocktissuecultureproductionusingreducedgrapheneoxidesilvercopperandsilverseleniumnanocomposites AT janacechova eliminationofcurtobacteriumspstraina7m15acontaminantinprunusrootstocktissuecultureproductionusingreducedgrapheneoxidesilvercopperandsilverseleniumnanocomposites AT zuzanabytesnikova eliminationofcurtobacteriumspstraina7m15acontaminantinprunusrootstocktissuecultureproductionusingreducedgrapheneoxidesilvercopperandsilverseleniumnanocomposites AT lukasrichtera eliminationofcurtobacteriumspstraina7m15acontaminantinprunusrootstocktissuecultureproductionusingreducedgrapheneoxidesilvercopperandsilverseleniumnanocomposites AT tomaskiss eliminationofcurtobacteriumspstraina7m15acontaminantinprunusrootstocktissuecultureproductionusingreducedgrapheneoxidesilvercopperandsilverseleniumnanocomposites AT aleseichmeier eliminationofcurtobacteriumspstraina7m15acontaminantinprunusrootstocktissuecultureproductionusingreducedgrapheneoxidesilvercopperandsilverseleniumnanocomposites AT miroslavbaranek eliminationofcurtobacteriumspstraina7m15acontaminantinprunusrootstocktissuecultureproductionusingreducedgrapheneoxidesilvercopperandsilverseleniumnanocomposites |