Bioinformatic analysis of differentially expressed genes in porcine intestinal epithelial cells infected with transmissible gastroenteritis virus
Transmissible Gastroenteritis Virus (TGEV) infection is one of the leading causes of diarrhea in piglets. Intestinal immune system plays an important role in maintaining the intestinal mucosa"s integrity and resisting infection by pathogens. However, the relationship between the immune response...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2021-11-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2860 |
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| author | Xu-xuan ZHANG Guo-wei LAN Zhe WANG Chen-hao JIANG Jing XI Yao LI Jun QIAO Yan REN |
| author_facet | Xu-xuan ZHANG Guo-wei LAN Zhe WANG Chen-hao JIANG Jing XI Yao LI Jun QIAO Yan REN |
| author_sort | Xu-xuan ZHANG |
| collection | DOAJ |
| description | Transmissible Gastroenteritis Virus (TGEV) infection is one of the leading causes of diarrhea in piglets. Intestinal immune system plays an important role in maintaining the intestinal mucosa"s integrity and resisting infection by pathogens. However, the relationship between the immune response induced by TGEV infection and disease progression is unclear. Using the microarray data set GSE41756 from the Gene Expression Omnibus database, we analyzed porcine small intestinal epithelial cells at 6 and 12 h of TGEV infection. Differentially expressed genes (DEGs) were detected using the "limma" R package. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway enrichment, and the protein-protein interaction network analyses were performed with the detected DEGs. We found 56 DEGs (47 up-regulated and 9 down-regulated) after the intersection. Pathway enrichment analysis revealed that the DEGs were mainly associated with immune response, extracellular space, cytokine activity, and positive regulation of nuclear factor-κB import into the nucleus. This revealed the strong relationships among DEGs in the tumor necrosis factor signaling pathway, inflammatory bowel disease, and influenza A. Interleukin 6 (IL6), IL8, IL18, tumor necrosis factor, and toll-like receptor hub genes may play important roles during TGEV infection. |
| first_indexed | 2024-03-13T04:39:27Z |
| format | Article |
| id | doaj.art-817854c75c744700a5a76b66e3998cd5 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:39:27Z |
| publishDate | 2021-11-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-817854c75c744700a5a76b66e3998cd52023-06-19T06:28:53ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512021-11-0127680380910.9775/kvfd.2021.263732860Bioinformatic analysis of differentially expressed genes in porcine intestinal epithelial cells infected with transmissible gastroenteritis virusXu-xuan ZHANG0Guo-wei LAN1Zhe WANG2Chen-hao JIANG3Jing XI4Yao LI5Jun QIAO6Yan REN7First Affiliated Hospital of School of Medicine, Shihezi University, Shihezi 832000, CHINASchool of Chemistry and Chemical Engineering, Shihezi University, Xinjiang, 832003, CHINAFirst Affiliated Hospital of School of Medicine, Shihezi University, Shihezi 832000, CHINAFirst Affiliated Hospital of School of Medicine, Shihezi University, Shihezi 832000, CHINASchool of Animal Science and Technology, Shihezi University, Shihezi 832000, CHINAFirst Affiliated Hospital of School of Medicine, Shihezi University, Shihezi 832000, CHINASchool of Animal Science and Technology, Shihezi University, Shihezi 832000, CHINAFirst Affiliated Hospital of School of Medicine, Shihezi University, Shihezi 832000, CHINATransmissible Gastroenteritis Virus (TGEV) infection is one of the leading causes of diarrhea in piglets. Intestinal immune system plays an important role in maintaining the intestinal mucosa"s integrity and resisting infection by pathogens. However, the relationship between the immune response induced by TGEV infection and disease progression is unclear. Using the microarray data set GSE41756 from the Gene Expression Omnibus database, we analyzed porcine small intestinal epithelial cells at 6 and 12 h of TGEV infection. Differentially expressed genes (DEGs) were detected using the "limma" R package. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes pathway enrichment, and the protein-protein interaction network analyses were performed with the detected DEGs. We found 56 DEGs (47 up-regulated and 9 down-regulated) after the intersection. Pathway enrichment analysis revealed that the DEGs were mainly associated with immune response, extracellular space, cytokine activity, and positive regulation of nuclear factor-κB import into the nucleus. This revealed the strong relationships among DEGs in the tumor necrosis factor signaling pathway, inflammatory bowel disease, and influenza A. Interleukin 6 (IL6), IL8, IL18, tumor necrosis factor, and toll-like receptor hub genes may play important roles during TGEV infection.https://vetdergikafkas.org/pdf.php?id=2860degsimmune responsetgevtnftlr2 |
| spellingShingle | Xu-xuan ZHANG Guo-wei LAN Zhe WANG Chen-hao JIANG Jing XI Yao LI Jun QIAO Yan REN Bioinformatic analysis of differentially expressed genes in porcine intestinal epithelial cells infected with transmissible gastroenteritis virus Kafkas Universitesi Veteriner Fakültesi Dergisi degs immune response tgev tnf tlr2 |
| title | Bioinformatic analysis of differentially expressed genes in porcine intestinal epithelial cells infected with transmissible gastroenteritis virus |
| title_full | Bioinformatic analysis of differentially expressed genes in porcine intestinal epithelial cells infected with transmissible gastroenteritis virus |
| title_fullStr | Bioinformatic analysis of differentially expressed genes in porcine intestinal epithelial cells infected with transmissible gastroenteritis virus |
| title_full_unstemmed | Bioinformatic analysis of differentially expressed genes in porcine intestinal epithelial cells infected with transmissible gastroenteritis virus |
| title_short | Bioinformatic analysis of differentially expressed genes in porcine intestinal epithelial cells infected with transmissible gastroenteritis virus |
| title_sort | bioinformatic analysis of differentially expressed genes in porcine intestinal epithelial cells infected with transmissible gastroenteritis virus |
| topic | degs immune response tgev tnf tlr2 |
| url | https://vetdergikafkas.org/pdf.php?id=2860 |
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