The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR
Abstract Coptidis Rhizoma binds to the membrane receptors on hPDLSC/CMC, and the active ingredient Berberine (BER) that can be extracted from it may promote the proliferation and osteogenesis of periodontal ligament stem cells (hPDLSC). The membrane receptor that binds with BER on the cell surface o...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2018-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-21116-3 |
| _version_ | 1819202654296866816 |
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| author | Jin Liu Xiaodan Zhao Dandan Pei Guo Sun Ye Li Chunhui Zhu Cui Qiang Junyi Sun Jianfeng Shi Yan Dong Jianzhong Gou Sicen Wang Ang Li |
| author_facet | Jin Liu Xiaodan Zhao Dandan Pei Guo Sun Ye Li Chunhui Zhu Cui Qiang Junyi Sun Jianfeng Shi Yan Dong Jianzhong Gou Sicen Wang Ang Li |
| author_sort | Jin Liu |
| collection | DOAJ |
| description | Abstract Coptidis Rhizoma binds to the membrane receptors on hPDLSC/CMC, and the active ingredient Berberine (BER) that can be extracted from it may promote the proliferation and osteogenesis of periodontal ligament stem cells (hPDLSC). The membrane receptor that binds with BER on the cell surface of hPDLSC, the mechanism of direct interaction between BER and hPDLSC, and the related signal pathway are not yet clear. In this research, EGFR was screened as the affinity membrane receptor between BER and hPDLSC, through retention on CMC, competition with BER and by using a molecular docking simulation score. At the same time, the MAPK PCR Array was selected to screen the target genes that changed when hPDLSC was simulated by BER. In conclusion, BER may bind to EGFR on the cell membrane of hPDLSC so the intracellular ERK signalling pathways activate, and nuclear-related genes of FOS change, resulting in the effect of osteogenesis on PDLSC. |
| first_indexed | 2024-12-23T04:07:27Z |
| format | Article |
| id | doaj.art-817ba142af204852a14af24899beff10 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-23T04:07:27Z |
| publishDate | 2018-02-01 |
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| spelling | doaj.art-817ba142af204852a14af24899beff102022-12-21T18:00:36ZengNature PortfolioScientific Reports2045-23222018-02-018111010.1038/s41598-018-21116-3The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFRJin Liu0Xiaodan Zhao1Dandan Pei2Guo Sun3Ye Li4Chunhui Zhu5Cui Qiang6Junyi Sun7Jianfeng Shi8Yan Dong9Jianzhong Gou10Sicen Wang11Ang Li12Key Laboratory of Shannxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong UniversityKey Laboratory of Shannxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong UniversityKey Laboratory of Shannxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong UniversityKey Laboratory of Shannxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong UniversityKey Laboratory of Shannxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong UniversityKey Laboratory of Shannxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong UniversityKey Laboratory of Shannxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong UniversityKey Laboratory of Shannxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong UniversityKey Laboratory of Shannxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong UniversityThe Second Affiliated Hospital, Xi’an Jiaotong UniversityKey Laboratory of Shannxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong UniversitySchool of Pharmacy, Xi’an Jiaotong UniversityKey Laboratory of Shannxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong UniversityAbstract Coptidis Rhizoma binds to the membrane receptors on hPDLSC/CMC, and the active ingredient Berberine (BER) that can be extracted from it may promote the proliferation and osteogenesis of periodontal ligament stem cells (hPDLSC). The membrane receptor that binds with BER on the cell surface of hPDLSC, the mechanism of direct interaction between BER and hPDLSC, and the related signal pathway are not yet clear. In this research, EGFR was screened as the affinity membrane receptor between BER and hPDLSC, through retention on CMC, competition with BER and by using a molecular docking simulation score. At the same time, the MAPK PCR Array was selected to screen the target genes that changed when hPDLSC was simulated by BER. In conclusion, BER may bind to EGFR on the cell membrane of hPDLSC so the intracellular ERK signalling pathways activate, and nuclear-related genes of FOS change, resulting in the effect of osteogenesis on PDLSC.https://doi.org/10.1038/s41598-018-21116-3 |
| spellingShingle | Jin Liu Xiaodan Zhao Dandan Pei Guo Sun Ye Li Chunhui Zhu Cui Qiang Junyi Sun Jianfeng Shi Yan Dong Jianzhong Gou Sicen Wang Ang Li The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR Scientific Reports |
| title | The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR |
| title_full | The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR |
| title_fullStr | The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR |
| title_full_unstemmed | The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR |
| title_short | The promotion function of Berberine for osteogenic differentiation of human periodontal ligament stem cells via ERK-FOS pathway mediated by EGFR |
| title_sort | promotion function of berberine for osteogenic differentiation of human periodontal ligament stem cells via erk fos pathway mediated by egfr |
| url | https://doi.org/10.1038/s41598-018-21116-3 |
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