Rv1460, a SufR homologue, is a repressor of the suf operon in Mycobacterium tuberculosis.
Iron-sulphur (Fe-S) clusters are ubiquitous co-factors which require multi-protein systems for their synthesis. In Mycobacterium tuberculosis, the Rv1460-Rv1461-Rv1462-Rv1463-csd-Rv1465-Rv1466 operon (suf operon) encodes the primary Fe-S cluster biogenesis system. The first gene in this operon, Rv14...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0200145&type=printable |
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| author | Danicke Willemse Brandon Weber Laura Masino Robin M Warren Salvatore Adinolfi Annalisa Pastore Monique J Williams |
| author_facet | Danicke Willemse Brandon Weber Laura Masino Robin M Warren Salvatore Adinolfi Annalisa Pastore Monique J Williams |
| author_sort | Danicke Willemse |
| collection | DOAJ |
| description | Iron-sulphur (Fe-S) clusters are ubiquitous co-factors which require multi-protein systems for their synthesis. In Mycobacterium tuberculosis, the Rv1460-Rv1461-Rv1462-Rv1463-csd-Rv1465-Rv1466 operon (suf operon) encodes the primary Fe-S cluster biogenesis system. The first gene in this operon, Rv1460, shares homology with the cyanobacterial SufR, which functions as a transcriptional repressor of the sufBCDS operon. Rv1460's function in M. tuberculosis has however not been determined. In this study, we demonstrate that M. tuberculosis mutants lacking a functional Rv1460 protein are impaired for growth under standard culture conditions. Elevated expression of Rv1460 and Rv1461 was observed in the mutant, implicating Rv1460 in the regulation of the suf operon. Binding of an Fe-S cluster to purified recombinant Rv1460 was confirmed by UV-visible spectroscopy and circular dichroism. Furthermore, three conserved cysteine residues, C203, C216 and C244, proposed to provide ligands for the coordination of an Fe-S cluster, were shown to be required for the function of Rv1460 in M. tuberculosis. Rv1460 therefore seems to be functionally analogous to cyanobacterial SufR. |
| first_indexed | 2024-12-20T08:43:51Z |
| format | Article |
| id | doaj.art-817f88cd0e8641d594c86fef20b18247 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T14:15:24Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-817f88cd0e8641d594c86fef20b182472025-02-27T05:34:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01137e020014510.1371/journal.pone.0200145Rv1460, a SufR homologue, is a repressor of the suf operon in Mycobacterium tuberculosis.Danicke WillemseBrandon WeberLaura MasinoRobin M WarrenSalvatore AdinolfiAnnalisa PastoreMonique J WilliamsIron-sulphur (Fe-S) clusters are ubiquitous co-factors which require multi-protein systems for their synthesis. In Mycobacterium tuberculosis, the Rv1460-Rv1461-Rv1462-Rv1463-csd-Rv1465-Rv1466 operon (suf operon) encodes the primary Fe-S cluster biogenesis system. The first gene in this operon, Rv1460, shares homology with the cyanobacterial SufR, which functions as a transcriptional repressor of the sufBCDS operon. Rv1460's function in M. tuberculosis has however not been determined. In this study, we demonstrate that M. tuberculosis mutants lacking a functional Rv1460 protein are impaired for growth under standard culture conditions. Elevated expression of Rv1460 and Rv1461 was observed in the mutant, implicating Rv1460 in the regulation of the suf operon. Binding of an Fe-S cluster to purified recombinant Rv1460 was confirmed by UV-visible spectroscopy and circular dichroism. Furthermore, three conserved cysteine residues, C203, C216 and C244, proposed to provide ligands for the coordination of an Fe-S cluster, were shown to be required for the function of Rv1460 in M. tuberculosis. Rv1460 therefore seems to be functionally analogous to cyanobacterial SufR.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0200145&type=printable |
| spellingShingle | Danicke Willemse Brandon Weber Laura Masino Robin M Warren Salvatore Adinolfi Annalisa Pastore Monique J Williams Rv1460, a SufR homologue, is a repressor of the suf operon in Mycobacterium tuberculosis. PLoS ONE |
| title | Rv1460, a SufR homologue, is a repressor of the suf operon in Mycobacterium tuberculosis. |
| title_full | Rv1460, a SufR homologue, is a repressor of the suf operon in Mycobacterium tuberculosis. |
| title_fullStr | Rv1460, a SufR homologue, is a repressor of the suf operon in Mycobacterium tuberculosis. |
| title_full_unstemmed | Rv1460, a SufR homologue, is a repressor of the suf operon in Mycobacterium tuberculosis. |
| title_short | Rv1460, a SufR homologue, is a repressor of the suf operon in Mycobacterium tuberculosis. |
| title_sort | rv1460 a sufr homologue is a repressor of the suf operon in mycobacterium tuberculosis |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0200145&type=printable |
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