Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in Indonesia

The objective of this study was to discover and identify the effect of SNP of follicle-stimulating hormone receptor (FSHR) gen on prolific traits in Kejobong (KJ) and Etawah grade (EG) doe. A total of 15 blood samples were taken from 11 KJ and 4 EG with various parity and type of birth. The FSHR gen...

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Main Authors: E. T. Setiatin, D. A. Lestari, D. W. Harjanti, A. Setiaji, E. Kurnianto
Format: Article
Language:English
Published: Diponegoro University, Faculty of Animal and Agricultural Sciences 2023-03-01
Series:Journal of the Indonesian Tropical Animal Agriculture
Subjects:
Online Access:https://ejournal.undip.ac.id/index.php/jitaa/article/view/50343
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author E. T. Setiatin
D. A. Lestari
D. W. Harjanti
A. Setiaji
E. Kurnianto
author_facet E. T. Setiatin
D. A. Lestari
D. W. Harjanti
A. Setiaji
E. Kurnianto
author_sort E. T. Setiatin
collection DOAJ
description The objective of this study was to discover and identify the effect of SNP of follicle-stimulating hormone receptor (FSHR) gen on prolific traits in Kejobong (KJ) and Etawah grade (EG) doe. A total of 15 blood samples were taken from 11 KJ and 4 EG with various parity and type of birth. The FSHR gene was amplified from the DNA template by Polymerase Chain Reaction (PCR); the PCR products were then sequenced to determine Single Nucleotide Polymorphisms (SNP). Results showed that 3 SNPs were identified, and those SNPs altered amino acid sequence formed 6 haplotypes and divided the doe based on the type of birth. In conclusion, SNPs identified in this study are associated with a prolific trait that can be used as a genetic marker and haplotype 3, 4, and 5 showed the highest prolific on KJ and EG doe.
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spelling doaj.art-8181835ced2b4babbb68613c8d3c9c952023-03-17T09:05:30ZengDiponegoro University, Faculty of Animal and Agricultural SciencesJournal of the Indonesian Tropical Animal Agriculture2087-82732460-62782023-03-01481283610.14710/jitaa.48.1.28-3621720Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in IndonesiaE. T. Setiatin0D. A. Lestari1D. W. Harjanti2A. Setiaji3E. Kurnianto4Department of Animal Science, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro, IndonesiaDepartment of Animal Science, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro, IndonesiaDepartment of Animal Science, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro, IndonesiaDepartment of Animal Science, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro, IndonesiaDepartment of Animal Science, Faculty of Animal and Agricultural Sciences, Universitas Diponegoro, IndonesiaThe objective of this study was to discover and identify the effect of SNP of follicle-stimulating hormone receptor (FSHR) gen on prolific traits in Kejobong (KJ) and Etawah grade (EG) doe. A total of 15 blood samples were taken from 11 KJ and 4 EG with various parity and type of birth. The FSHR gene was amplified from the DNA template by Polymerase Chain Reaction (PCR); the PCR products were then sequenced to determine Single Nucleotide Polymorphisms (SNP). Results showed that 3 SNPs were identified, and those SNPs altered amino acid sequence formed 6 haplotypes and divided the doe based on the type of birth. In conclusion, SNPs identified in this study are associated with a prolific trait that can be used as a genetic marker and haplotype 3, 4, and 5 showed the highest prolific on KJ and EG doe.https://ejournal.undip.ac.id/index.php/jitaa/article/view/50343fshrgenetic markerlocal goatprolificsnps
spellingShingle E. T. Setiatin
D. A. Lestari
D. W. Harjanti
A. Setiaji
E. Kurnianto
Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in Indonesia
Journal of the Indonesian Tropical Animal Agriculture
fshr
genetic marker
local goat
prolific
snps
title Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in Indonesia
title_full Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in Indonesia
title_fullStr Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in Indonesia
title_full_unstemmed Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in Indonesia
title_short Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in Indonesia
title_sort follicle stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in indonesia
topic fshr
genetic marker
local goat
prolific
snps
url https://ejournal.undip.ac.id/index.php/jitaa/article/view/50343
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AT asetiaji folliclestimulatinghormonereceptorgeneexplorationaspossiblemarkersforprolifictraitoflocalgoatinindonesia
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