TETRASPANIN 8-1 from Phaseolus vulgaris plays a key role during mutualistic interactions

Arbuscular mycorrhizal (AM) fungi and rhizobia form two of the most important plant-microbe associations for the assimilation of phosphorus (P) and nitrogen (N). Symbiont-derived signals are able to coordinate the infection process by triggering multiple responses in the plant root, such as calcium...

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Main Authors: Thelma J. Parra-Aguilar, Luis G. Sarmiento-López, Olivia Santana, Juan Elías Olivares, Edgar Pascual-Morales, Saul Jiménez-Jiménez, Andrea Quero-Hostos, Janet Palacios-Martínez, Ana I. Chávez-Martínez, Luis Cárdenas
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-07-01
Series:Frontiers in Plant Science
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Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2023.1152493/full
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author Thelma J. Parra-Aguilar
Luis G. Sarmiento-López
Olivia Santana
Juan Elías Olivares
Edgar Pascual-Morales
Saul Jiménez-Jiménez
Andrea Quero-Hostos
Janet Palacios-Martínez
Ana I. Chávez-Martínez
Luis Cárdenas
author_facet Thelma J. Parra-Aguilar
Luis G. Sarmiento-López
Olivia Santana
Juan Elías Olivares
Edgar Pascual-Morales
Saul Jiménez-Jiménez
Andrea Quero-Hostos
Janet Palacios-Martínez
Ana I. Chávez-Martínez
Luis Cárdenas
author_sort Thelma J. Parra-Aguilar
collection DOAJ
description Arbuscular mycorrhizal (AM) fungi and rhizobia form two of the most important plant-microbe associations for the assimilation of phosphorus (P) and nitrogen (N). Symbiont-derived signals are able to coordinate the infection process by triggering multiple responses in the plant root, such as calcium influxes and oscillations, increased reactive oxygen species (ROS), cytoskeletal rearrangements and altered gene expression. An examination was made of the role of tetraspanins, which are transmembrane proteins that self-organize into tetraspanin web regions, where they recruit specific proteins into platforms required for signal transduction, membrane fusion, cell trafficking, and ROS generation. In plant cells, tetraspanins are scaffolding proteins associated with root radial patterning, biotic and abiotic stress responses, cell fate determination, plasmodesmata and hormonal regulation. Some plant tetraspanins, such as Arabidopsis thaliana TETRASPANIN 8 and TETRASPANIN 9 (AtTET8 and AtTET9) are associated with exosomes during inter-kingdom communication. In this study, a homolog of AtTET8, PvTET8-1, in common bean (Phaseolus vulgaris L. var. Negro Jamapa) was examined in roots during interactions with Rhizobium tropici and Rhizophagus irregularis. The promoter of PvTET8-1 contained several cis-acting regulatory DNA elements potentially related to mutualistic interactions, and PvTET8-1 was transcriptionally activated during AM fungal and rhizobial associations. Silencing it decreased the size and number of nodules, nitrogen fixation, and mycorrhizal arbuscule formation, whereas overexpressing it increased the size and number of nodules, and mycorrhizal arbuscule formation but decreased nitrogen fixation. PvTET8-1 appears to be an important element in both of these mutualistic interactions, perhaps through its interaction with NADPH oxidase and the generation of ROS during the infection processes.
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spelling doaj.art-818386777827489797d11b3f512a7efa2023-07-03T08:18:47ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-07-011410.3389/fpls.2023.11524931152493TETRASPANIN 8-1 from Phaseolus vulgaris plays a key role during mutualistic interactionsThelma J. Parra-Aguilar0Luis G. Sarmiento-López1Olivia Santana2Juan Elías Olivares3Edgar Pascual-Morales4Saul Jiménez-Jiménez5Andrea Quero-Hostos6Janet Palacios-Martínez7Ana I. Chávez-Martínez8Luis Cárdenas9Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, MexicoDepartamento de Biotecnología Agrícola, Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional Unidad Sinaloa-Instituto Politécnico Nacional, Guasave, Sinaloa, MexicoDepartamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, MexicoDepartamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, MexicoDepartamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, MexicoDepartamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, MexicoDepartamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, MexicoDepartamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, MexicoDepartamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, MexicoDepartamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, MexicoArbuscular mycorrhizal (AM) fungi and rhizobia form two of the most important plant-microbe associations for the assimilation of phosphorus (P) and nitrogen (N). Symbiont-derived signals are able to coordinate the infection process by triggering multiple responses in the plant root, such as calcium influxes and oscillations, increased reactive oxygen species (ROS), cytoskeletal rearrangements and altered gene expression. An examination was made of the role of tetraspanins, which are transmembrane proteins that self-organize into tetraspanin web regions, where they recruit specific proteins into platforms required for signal transduction, membrane fusion, cell trafficking, and ROS generation. In plant cells, tetraspanins are scaffolding proteins associated with root radial patterning, biotic and abiotic stress responses, cell fate determination, plasmodesmata and hormonal regulation. Some plant tetraspanins, such as Arabidopsis thaliana TETRASPANIN 8 and TETRASPANIN 9 (AtTET8 and AtTET9) are associated with exosomes during inter-kingdom communication. In this study, a homolog of AtTET8, PvTET8-1, in common bean (Phaseolus vulgaris L. var. Negro Jamapa) was examined in roots during interactions with Rhizobium tropici and Rhizophagus irregularis. The promoter of PvTET8-1 contained several cis-acting regulatory DNA elements potentially related to mutualistic interactions, and PvTET8-1 was transcriptionally activated during AM fungal and rhizobial associations. Silencing it decreased the size and number of nodules, nitrogen fixation, and mycorrhizal arbuscule formation, whereas overexpressing it increased the size and number of nodules, and mycorrhizal arbuscule formation but decreased nitrogen fixation. PvTET8-1 appears to be an important element in both of these mutualistic interactions, perhaps through its interaction with NADPH oxidase and the generation of ROS during the infection processes.https://www.frontiersin.org/articles/10.3389/fpls.2023.1152493/fullnodulationmycorrhizationtetraspaninnodulearbusculeROS
spellingShingle Thelma J. Parra-Aguilar
Luis G. Sarmiento-López
Olivia Santana
Juan Elías Olivares
Edgar Pascual-Morales
Saul Jiménez-Jiménez
Andrea Quero-Hostos
Janet Palacios-Martínez
Ana I. Chávez-Martínez
Luis Cárdenas
TETRASPANIN 8-1 from Phaseolus vulgaris plays a key role during mutualistic interactions
Frontiers in Plant Science
nodulation
mycorrhization
tetraspanin
nodule
arbuscule
ROS
title TETRASPANIN 8-1 from Phaseolus vulgaris plays a key role during mutualistic interactions
title_full TETRASPANIN 8-1 from Phaseolus vulgaris plays a key role during mutualistic interactions
title_fullStr TETRASPANIN 8-1 from Phaseolus vulgaris plays a key role during mutualistic interactions
title_full_unstemmed TETRASPANIN 8-1 from Phaseolus vulgaris plays a key role during mutualistic interactions
title_short TETRASPANIN 8-1 from Phaseolus vulgaris plays a key role during mutualistic interactions
title_sort tetraspanin 8 1 from phaseolus vulgaris plays a key role during mutualistic interactions
topic nodulation
mycorrhization
tetraspanin
nodule
arbuscule
ROS
url https://www.frontiersin.org/articles/10.3389/fpls.2023.1152493/full
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