Characterization of <i>JsWOX1</i> and <i>JsWOX4</i> during Callus and Root Induction in the Shrub Species <i>Jasminum sambac</i>
Plant regeneration <i>in vitro</i> and the underlying molecular regulatory network are of great interest to developmental biology, and have potential applications in agriculture and biotechnology. Cell growth and re-differentiation during <i>de novo</i> organogenesis require...
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| Format: | Article |
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MDPI AG
2019-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/8/4/79 |
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| author | Ying Lu Zhuoyi Liu Meiling Lyu Yuan Yuan Binghua Wu |
| author_facet | Ying Lu Zhuoyi Liu Meiling Lyu Yuan Yuan Binghua Wu |
| author_sort | Ying Lu |
| collection | DOAJ |
| description | Plant regeneration <i>in vitro</i> and the underlying molecular regulatory network are of great interest to developmental biology, and have potential applications in agriculture and biotechnology. Cell growth and re-differentiation during <i>de novo</i> organogenesis require the activation and reprogramming of stem cells within the stem cell niche of the tissues. The WUSCHEL-related homeobox (WOX) factors play important roles in the maintenance and regulation of plant stem cells and are involved in many developmental processes. However, in woody species such as the <i>Jasminum sambac,</i> little is known about the involvement of <i>WOX</i> genes in <i>de novo</i> organogenesis. Here we show that two <i>WOXs</i>, <i>JsWOX4</i> and <i>JsWOX1</i>, are implicated in callus proliferation and root regeneration, respectively. The expression of both, together with another member <i>JsWOX13</i>, are upregulated during later stage of callus formation. The <i>JsWOX4</i> is associated with callus proliferation, or cell division during the redifferentiation. The overexpression of this gene results in up-regulation of <i>JsWOX13</i> and another homeobox gene. The <i>JsWOX1</i> plays a role in root primordium initiation, as its overexpression leads to more rooty calli and more roots per callus. <i>JsWOX1</i> also possibly acts upstream of <i>JsWOX4</i> and <i>JsWOX13</i> transcriptionally. Our results provide further evidence regarding the functions of <i>WOX</i> genes in organogenesis in a woody plant. |
| first_indexed | 2024-12-24T03:22:52Z |
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| id | doaj.art-81854a08ba8d4c889f19436a8c68cd75 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-24T03:22:52Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj.art-81854a08ba8d4c889f19436a8c68cd752022-12-21T17:17:26ZengMDPI AGPlants2223-77472019-03-01847910.3390/plants8040079plants8040079Characterization of <i>JsWOX1</i> and <i>JsWOX4</i> during Callus and Root Induction in the Shrub Species <i>Jasminum sambac</i>Ying Lu0Zhuoyi Liu1Meiling Lyu2Yuan Yuan3Binghua Wu4Fujian Provincial Key Laboratory of Plant Functional Biology, College of Horticulture, Fujian A & F University, Fuzhou 350002, ChinaFujian Provincial Key Laboratory of Plant Functional Biology, College of Horticulture, Fujian A & F University, Fuzhou 350002, ChinaFujian Provincial Key Laboratory of Plant Functional Biology, College of Horticulture, Fujian A & F University, Fuzhou 350002, ChinaFujian Provincial Key Laboratory of Plant Functional Biology, College of Horticulture, Fujian A & F University, Fuzhou 350002, ChinaFujian Provincial Key Laboratory of Plant Functional Biology, College of Horticulture, Fujian A & F University, Fuzhou 350002, ChinaPlant regeneration <i>in vitro</i> and the underlying molecular regulatory network are of great interest to developmental biology, and have potential applications in agriculture and biotechnology. Cell growth and re-differentiation during <i>de novo</i> organogenesis require the activation and reprogramming of stem cells within the stem cell niche of the tissues. The WUSCHEL-related homeobox (WOX) factors play important roles in the maintenance and regulation of plant stem cells and are involved in many developmental processes. However, in woody species such as the <i>Jasminum sambac,</i> little is known about the involvement of <i>WOX</i> genes in <i>de novo</i> organogenesis. Here we show that two <i>WOXs</i>, <i>JsWOX4</i> and <i>JsWOX1</i>, are implicated in callus proliferation and root regeneration, respectively. The expression of both, together with another member <i>JsWOX13</i>, are upregulated during later stage of callus formation. The <i>JsWOX4</i> is associated with callus proliferation, or cell division during the redifferentiation. The overexpression of this gene results in up-regulation of <i>JsWOX13</i> and another homeobox gene. The <i>JsWOX1</i> plays a role in root primordium initiation, as its overexpression leads to more rooty calli and more roots per callus. <i>JsWOX1</i> also possibly acts upstream of <i>JsWOX4</i> and <i>JsWOX13</i> transcriptionally. Our results provide further evidence regarding the functions of <i>WOX</i> genes in organogenesis in a woody plant.https://www.mdpi.com/2223-7747/8/4/79callus<i>de novo</i> organogenesisWUSCHEL-related homeobox (WOX) transcription factors<i>Jasminum sambac</i>transgenic |
| spellingShingle | Ying Lu Zhuoyi Liu Meiling Lyu Yuan Yuan Binghua Wu Characterization of <i>JsWOX1</i> and <i>JsWOX4</i> during Callus and Root Induction in the Shrub Species <i>Jasminum sambac</i> Plants callus <i>de novo</i> organogenesis WUSCHEL-related homeobox (WOX) transcription factors <i>Jasminum sambac</i> transgenic |
| title | Characterization of <i>JsWOX1</i> and <i>JsWOX4</i> during Callus and Root Induction in the Shrub Species <i>Jasminum sambac</i> |
| title_full | Characterization of <i>JsWOX1</i> and <i>JsWOX4</i> during Callus and Root Induction in the Shrub Species <i>Jasminum sambac</i> |
| title_fullStr | Characterization of <i>JsWOX1</i> and <i>JsWOX4</i> during Callus and Root Induction in the Shrub Species <i>Jasminum sambac</i> |
| title_full_unstemmed | Characterization of <i>JsWOX1</i> and <i>JsWOX4</i> during Callus and Root Induction in the Shrub Species <i>Jasminum sambac</i> |
| title_short | Characterization of <i>JsWOX1</i> and <i>JsWOX4</i> during Callus and Root Induction in the Shrub Species <i>Jasminum sambac</i> |
| title_sort | characterization of i jswox1 i and i jswox4 i during callus and root induction in the shrub species i jasminum sambac i |
| topic | callus <i>de novo</i> organogenesis WUSCHEL-related homeobox (WOX) transcription factors <i>Jasminum sambac</i> transgenic |
| url | https://www.mdpi.com/2223-7747/8/4/79 |
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