Effects of flaxseed oil on anti-oxidative system and membrane deformation of human peripheral blood erythrocytes in high glucose level
<p>Abstract</p> <p>Background</p> <p>The erythrocyte membrane lesion is a serious diabetic complication. A number of studies suggested that n-3 fatty acid could reduce lipid peroxidation and elevate α- or γ-tocopherol contents in membrane of erythrocytes. However, evide...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-07-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://www.lipidworld.com/content/11/1/88 |
| _version_ | 1818807848245657600 |
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| author | Yang Wei Fu Juan Yu Miao Huang Qingde Wang Di Xu Jiqu Deng Qianchun Yao Ping Huang Fenghong Liu Liegang |
| author_facet | Yang Wei Fu Juan Yu Miao Huang Qingde Wang Di Xu Jiqu Deng Qianchun Yao Ping Huang Fenghong Liu Liegang |
| author_sort | Yang Wei |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The erythrocyte membrane lesion is a serious diabetic complication. A number of studies suggested that n-3 fatty acid could reduce lipid peroxidation and elevate α- or γ-tocopherol contents in membrane of erythrocytes. However, evidence regarding the protective effects of flaxseed oil, a natural product rich in n-3 fatty acid, on lipid peroxidation, antioxidative capacity and membrane deformation of erythrocytes exposed to high glucose is limited.</p> <p>Methods</p> <p>Human peripheral blood erythrocytes were isolated and treated with 50 mM glucose to mimic hyperglycemia in the absence or presence of three different doses of flaxseed oil (50, 100 or 200 μM) in the culture medium for 24 h. The malondialdehyde (MDA) and L-glutathione (GSH) were measured by HPLC and LC/MS respectively. The phospholipids symmetry and membrane fatty acid composition of human erythrocytes were detected by flow cytometry and gas chromatograph (GC). The morphology of human erythrocyte was illuminated by ultra scanning electron microscopy.</p> <p>Results</p> <p>Flaxseed oil attenuated hyperglycemia-induced increase of MDA and decrease of GSH in human erythrocytes. Human erythrocytes treated with flaxseed oil contained higher C22:5 and C22:6 than those in the 50 mM glucose control group, indicating that flaxseed oil could reduce lipid asymmetric distribution and membrane perturbation. The ultra scanning electron microscopy and flow cytometer have also indicated that flaxseed oil could protect the membrane of human erythrocytes from deformation at high glucose level.</p> <p>Conclusion</p> <p>The flaxseed oil supplementation may prevent lipid peroxidation and membrane dysfunction of human erythrocytes in hyperglycemia.</p> |
| first_indexed | 2024-12-18T19:32:11Z |
| format | Article |
| id | doaj.art-818b546f84214693b774d75d65417e2f |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-18T19:32:11Z |
| publishDate | 2012-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-818b546f84214693b774d75d65417e2f2022-12-21T20:55:41ZengBMCLipids in Health and Disease1476-511X2012-07-011118810.1186/1476-511X-11-88Effects of flaxseed oil on anti-oxidative system and membrane deformation of human peripheral blood erythrocytes in high glucose levelYang WeiFu JuanYu MiaoHuang QingdeWang DiXu JiquDeng QianchunYao PingHuang FenghongLiu Liegang<p>Abstract</p> <p>Background</p> <p>The erythrocyte membrane lesion is a serious diabetic complication. A number of studies suggested that n-3 fatty acid could reduce lipid peroxidation and elevate α- or γ-tocopherol contents in membrane of erythrocytes. However, evidence regarding the protective effects of flaxseed oil, a natural product rich in n-3 fatty acid, on lipid peroxidation, antioxidative capacity and membrane deformation of erythrocytes exposed to high glucose is limited.</p> <p>Methods</p> <p>Human peripheral blood erythrocytes were isolated and treated with 50 mM glucose to mimic hyperglycemia in the absence or presence of three different doses of flaxseed oil (50, 100 or 200 μM) in the culture medium for 24 h. The malondialdehyde (MDA) and L-glutathione (GSH) were measured by HPLC and LC/MS respectively. The phospholipids symmetry and membrane fatty acid composition of human erythrocytes were detected by flow cytometry and gas chromatograph (GC). The morphology of human erythrocyte was illuminated by ultra scanning electron microscopy.</p> <p>Results</p> <p>Flaxseed oil attenuated hyperglycemia-induced increase of MDA and decrease of GSH in human erythrocytes. Human erythrocytes treated with flaxseed oil contained higher C22:5 and C22:6 than those in the 50 mM glucose control group, indicating that flaxseed oil could reduce lipid asymmetric distribution and membrane perturbation. The ultra scanning electron microscopy and flow cytometer have also indicated that flaxseed oil could protect the membrane of human erythrocytes from deformation at high glucose level.</p> <p>Conclusion</p> <p>The flaxseed oil supplementation may prevent lipid peroxidation and membrane dysfunction of human erythrocytes in hyperglycemia.</p>http://www.lipidworld.com/content/11/1/88Flaxseed oilAntioxidationErythrocytesDiabetesMembrane |
| spellingShingle | Yang Wei Fu Juan Yu Miao Huang Qingde Wang Di Xu Jiqu Deng Qianchun Yao Ping Huang Fenghong Liu Liegang Effects of flaxseed oil on anti-oxidative system and membrane deformation of human peripheral blood erythrocytes in high glucose level Lipids in Health and Disease Flaxseed oil Antioxidation Erythrocytes Diabetes Membrane |
| title | Effects of flaxseed oil on anti-oxidative system and membrane deformation of human peripheral blood erythrocytes in high glucose level |
| title_full | Effects of flaxseed oil on anti-oxidative system and membrane deformation of human peripheral blood erythrocytes in high glucose level |
| title_fullStr | Effects of flaxseed oil on anti-oxidative system and membrane deformation of human peripheral blood erythrocytes in high glucose level |
| title_full_unstemmed | Effects of flaxseed oil on anti-oxidative system and membrane deformation of human peripheral blood erythrocytes in high glucose level |
| title_short | Effects of flaxseed oil on anti-oxidative system and membrane deformation of human peripheral blood erythrocytes in high glucose level |
| title_sort | effects of flaxseed oil on anti oxidative system and membrane deformation of human peripheral blood erythrocytes in high glucose level |
| topic | Flaxseed oil Antioxidation Erythrocytes Diabetes Membrane |
| url | http://www.lipidworld.com/content/11/1/88 |
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