A conserved RNA degradation complex required for spreading and epigenetic inheritance of heterochromatin
Heterochromatic domains containing histone H3 lysine 9 methylation (H3K9me) can be epigenetically inherited independently of underlying DNA sequence. To gain insight into the mechanisms that mediate epigenetic inheritance, we used a Schizosaccharomyces pombe inducible heterochromatin formation syste...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-06-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/54341 |
| _version_ | 1811180544149422080 |
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| author | Gergana Shipkovenska Alexander Durango Marian Kalocsay Steven P Gygi Danesh Moazed |
| author_facet | Gergana Shipkovenska Alexander Durango Marian Kalocsay Steven P Gygi Danesh Moazed |
| author_sort | Gergana Shipkovenska |
| collection | DOAJ |
| description | Heterochromatic domains containing histone H3 lysine 9 methylation (H3K9me) can be epigenetically inherited independently of underlying DNA sequence. To gain insight into the mechanisms that mediate epigenetic inheritance, we used a Schizosaccharomyces pombe inducible heterochromatin formation system to perform a genetic screen for mutations that abolish heterochromatin inheritance without affecting its establishment. We identified mutations in several pathways, including the conserved and essential Rix1-associated complex (henceforth the rixosome), which contains RNA endonuclease and polynucleotide kinase activities with known roles in ribosomal RNA processing. We show that the rixosome is required for spreading and epigenetic inheritance of heterochromatin in fission yeast. Viable rixosome mutations that disrupt its association with Swi6/HP1 fail to localize to heterochromatin, lead to accumulation of heterochromatic RNAs, and block spreading of H3K9me and silencing into actively transcribed regions. These findings reveal a new pathway for degradation of heterochromatic RNAs with essential roles in heterochromatin spreading and inheritance. |
| first_indexed | 2024-04-11T09:05:00Z |
| format | Article |
| id | doaj.art-81905807a78d47b6ae44e1920d3ae94d |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:05:00Z |
| publishDate | 2020-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-81905807a78d47b6ae44e1920d3ae94d2022-12-22T04:32:40ZengeLife Sciences Publications LtdeLife2050-084X2020-06-01910.7554/eLife.54341A conserved RNA degradation complex required for spreading and epigenetic inheritance of heterochromatinGergana Shipkovenska0https://orcid.org/0000-0001-6288-4401Alexander Durango1Marian Kalocsay2Steven P Gygi3Danesh Moazed4https://orcid.org/0000-0003-0321-6221Howard Hughes Medical Institute, Department of Cell Biology, Harvard Medical School, Boston, United StatesHoward Hughes Medical Institute, Department of Cell Biology, Harvard Medical School, Boston, United StatesDepartment of Cell Biology, Harvard Medical School, Boston, United StatesDepartment of Cell Biology, Harvard Medical School, Boston, United StatesHoward Hughes Medical Institute, Department of Cell Biology, Harvard Medical School, Boston, United StatesHeterochromatic domains containing histone H3 lysine 9 methylation (H3K9me) can be epigenetically inherited independently of underlying DNA sequence. To gain insight into the mechanisms that mediate epigenetic inheritance, we used a Schizosaccharomyces pombe inducible heterochromatin formation system to perform a genetic screen for mutations that abolish heterochromatin inheritance without affecting its establishment. We identified mutations in several pathways, including the conserved and essential Rix1-associated complex (henceforth the rixosome), which contains RNA endonuclease and polynucleotide kinase activities with known roles in ribosomal RNA processing. We show that the rixosome is required for spreading and epigenetic inheritance of heterochromatin in fission yeast. Viable rixosome mutations that disrupt its association with Swi6/HP1 fail to localize to heterochromatin, lead to accumulation of heterochromatic RNAs, and block spreading of H3K9me and silencing into actively transcribed regions. These findings reveal a new pathway for degradation of heterochromatic RNAs with essential roles in heterochromatin spreading and inheritance.https://elifesciences.org/articles/54341heterochromatinepigeneticsRNA degradationH3K9 methylationRix1rixosome |
| spellingShingle | Gergana Shipkovenska Alexander Durango Marian Kalocsay Steven P Gygi Danesh Moazed A conserved RNA degradation complex required for spreading and epigenetic inheritance of heterochromatin eLife heterochromatin epigenetics RNA degradation H3K9 methylation Rix1 rixosome |
| title | A conserved RNA degradation complex required for spreading and epigenetic inheritance of heterochromatin |
| title_full | A conserved RNA degradation complex required for spreading and epigenetic inheritance of heterochromatin |
| title_fullStr | A conserved RNA degradation complex required for spreading and epigenetic inheritance of heterochromatin |
| title_full_unstemmed | A conserved RNA degradation complex required for spreading and epigenetic inheritance of heterochromatin |
| title_short | A conserved RNA degradation complex required for spreading and epigenetic inheritance of heterochromatin |
| title_sort | conserved rna degradation complex required for spreading and epigenetic inheritance of heterochromatin |
| topic | heterochromatin epigenetics RNA degradation H3K9 methylation Rix1 rixosome |
| url | https://elifesciences.org/articles/54341 |
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