Quantitative Succinyl-Proteome Profiling of Turnip (<i>Brassica rapa</i> var. <i>rapa</i>) in Response to Cadmium Stress
Protein post-translational modification (PTM) is an efficient biological mechanism to regulate protein structure and function, but its role in plant responses to heavy metal stress is poorly understood. The present study performed quantitative succinyl-proteome profiling using liquid chromatography–...
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MDPI AG
2022-06-01
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| author | Xiong Li Danni Yang Yunqiang Yang Guihua Jin Xin Yin Yan Zheng Jianchu Xu Yongping Yang |
| author_facet | Xiong Li Danni Yang Yunqiang Yang Guihua Jin Xin Yin Yan Zheng Jianchu Xu Yongping Yang |
| author_sort | Xiong Li |
| collection | DOAJ |
| description | Protein post-translational modification (PTM) is an efficient biological mechanism to regulate protein structure and function, but its role in plant responses to heavy metal stress is poorly understood. The present study performed quantitative succinyl-proteome profiling using liquid chromatography–mass spectrometry analysis to explore the potential roles of lysine succinylation modification in turnip seedlings in response to cadmium (Cd) stress (20 μM) under hydroponic conditions over a short time period (0–8 h). A total of 547 succinylated sites on 256 proteins were identified in the shoots of turnip seedlings. These succinylated proteins participated in various biological processes (e.g., photosynthesis, tricarboxylic acid cycle, amino acid metabolism, and response to stimulation) that occurred in diverse cellular compartments according to the functional classification, subcellular localization, and protein interaction network analysis. Quantitative analysis showed that the intensities of nine succinylation sites on eight proteins were significantly altered (<i>p</i> < 0.05) in turnip shoots after 8 h of Cd stress. These differentially succinylated sites were highly conserved in Brassicaceae species and mostly located in the conserved domains of the proteins. Among them, a downregulated succinylation site (K150) in the glycolate oxidase protein (Gene0282600.1), an upregulated succinylation site (K396) in the catalase 3 protein (Gene0163880.1), and a downregulated succinylation site (K197) in the glutathione S-transferase protein (Gene0315380.1) may have contributed to the altered activity of the corresponding enzymes, which suggests that lysine succinylation affects the Cd detoxification process in turnip by regulating the H<sub>2</sub>O<sub>2</sub> accumulation and glutathione metabolism. These results provide novel insights into understanding Cd response mechanisms in plants and important protein modification information for the molecular-assisted breeding of <i>Brassica</i> varieties with distinct Cd tolerance and accumulation capacities. |
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| series | Cells |
| spelling | doaj.art-81973fecb341464c830dd1626abde22d2023-11-23T16:01:59ZengMDPI AGCells2073-44092022-06-011112194710.3390/cells11121947Quantitative Succinyl-Proteome Profiling of Turnip (<i>Brassica rapa</i> var. <i>rapa</i>) in Response to Cadmium StressXiong Li0Danni Yang1Yunqiang Yang2Guihua Jin3Xin Yin4Yan Zheng5Jianchu Xu6Yongping Yang7Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaYunnan Key Laboratory for Wild Plant Resources, Department of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaGermplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, ChinaProtein post-translational modification (PTM) is an efficient biological mechanism to regulate protein structure and function, but its role in plant responses to heavy metal stress is poorly understood. The present study performed quantitative succinyl-proteome profiling using liquid chromatography–mass spectrometry analysis to explore the potential roles of lysine succinylation modification in turnip seedlings in response to cadmium (Cd) stress (20 μM) under hydroponic conditions over a short time period (0–8 h). A total of 547 succinylated sites on 256 proteins were identified in the shoots of turnip seedlings. These succinylated proteins participated in various biological processes (e.g., photosynthesis, tricarboxylic acid cycle, amino acid metabolism, and response to stimulation) that occurred in diverse cellular compartments according to the functional classification, subcellular localization, and protein interaction network analysis. Quantitative analysis showed that the intensities of nine succinylation sites on eight proteins were significantly altered (<i>p</i> < 0.05) in turnip shoots after 8 h of Cd stress. These differentially succinylated sites were highly conserved in Brassicaceae species and mostly located in the conserved domains of the proteins. Among them, a downregulated succinylation site (K150) in the glycolate oxidase protein (Gene0282600.1), an upregulated succinylation site (K396) in the catalase 3 protein (Gene0163880.1), and a downregulated succinylation site (K197) in the glutathione S-transferase protein (Gene0315380.1) may have contributed to the altered activity of the corresponding enzymes, which suggests that lysine succinylation affects the Cd detoxification process in turnip by regulating the H<sub>2</sub>O<sub>2</sub> accumulation and glutathione metabolism. These results provide novel insights into understanding Cd response mechanisms in plants and important protein modification information for the molecular-assisted breeding of <i>Brassica</i> varieties with distinct Cd tolerance and accumulation capacities.https://www.mdpi.com/2073-4409/11/12/1947heavy metal pollution<i>Brassica</i>post-translational modificationdetoxificationmolecular breeding |
| spellingShingle | Xiong Li Danni Yang Yunqiang Yang Guihua Jin Xin Yin Yan Zheng Jianchu Xu Yongping Yang Quantitative Succinyl-Proteome Profiling of Turnip (<i>Brassica rapa</i> var. <i>rapa</i>) in Response to Cadmium Stress Cells heavy metal pollution <i>Brassica</i> post-translational modification detoxification molecular breeding |
| title | Quantitative Succinyl-Proteome Profiling of Turnip (<i>Brassica rapa</i> var. <i>rapa</i>) in Response to Cadmium Stress |
| title_full | Quantitative Succinyl-Proteome Profiling of Turnip (<i>Brassica rapa</i> var. <i>rapa</i>) in Response to Cadmium Stress |
| title_fullStr | Quantitative Succinyl-Proteome Profiling of Turnip (<i>Brassica rapa</i> var. <i>rapa</i>) in Response to Cadmium Stress |
| title_full_unstemmed | Quantitative Succinyl-Proteome Profiling of Turnip (<i>Brassica rapa</i> var. <i>rapa</i>) in Response to Cadmium Stress |
| title_short | Quantitative Succinyl-Proteome Profiling of Turnip (<i>Brassica rapa</i> var. <i>rapa</i>) in Response to Cadmium Stress |
| title_sort | quantitative succinyl proteome profiling of turnip i brassica rapa i var i rapa i in response to cadmium stress |
| topic | heavy metal pollution <i>Brassica</i> post-translational modification detoxification molecular breeding |
| url | https://www.mdpi.com/2073-4409/11/12/1947 |
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