Evolution of knockdown resistance (kdr) mutations of Aedes aegypti and Aedes albopictus in Hainan Island and Leizhou Peninsula, China
BackgroundAedes aegypti and Aedes albopictus are important vectors of human arboviruses, transmitting arboviral diseases such as yellow fever, dengue, chikungunya and Zika. These two mosquitoes coexist on Hainan Island and the Leizhou Peninsula in China. Over the past 40 years, the distribution of A...
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1265873/full |
| _version_ | 1797678099223019520 |
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| author | Minghui Zhao Minghui Zhao Xin Ran Dan Xing Yun Liao Wei Liu Yu Bai Qiang Zhang Kan Chen Lan Liu Mingyu Wu Zu Ma Jian Gao Hengduan Zhang Tongyan Zhao |
| author_facet | Minghui Zhao Minghui Zhao Xin Ran Dan Xing Yun Liao Wei Liu Yu Bai Qiang Zhang Kan Chen Lan Liu Mingyu Wu Zu Ma Jian Gao Hengduan Zhang Tongyan Zhao |
| author_sort | Minghui Zhao |
| collection | DOAJ |
| description | BackgroundAedes aegypti and Aedes albopictus are important vectors of human arboviruses, transmitting arboviral diseases such as yellow fever, dengue, chikungunya and Zika. These two mosquitoes coexist on Hainan Island and the Leizhou Peninsula in China. Over the past 40 years, the distribution of Ae. albopictus has gradually expanded in these areas, while the distribution of Ae. aegypti has declined dramatically mainly due to the ecological changes and some other factors such as heavy use of insecticide indoor based on endophagic bloodfeeding of the species.MethodsThis study focused on the knockdown resistance (kdr) genes of both mosquitoes, investigated their mutations, and analyzed their haplotype and evolutionary diversity combined with population genetic features based on the ND4/ND5 genes to further elucidate the molecular mechanisms underlying the development of insecticide resistance in both mosquitoes.ResultsThree mutations, S989P, V1016G and F1534C, were found to be present in Ae. aegypti populations, and the three mutations occurred synergistically. Multiple mutation types (F1534C/S/L/W) of the F1534 locus are found in Ae. albopictus populations, with the three common mutations F1534C, F1534S and F1534L all having multiple independent origins. The F1534W (TTC/TGG) mutation is thought to have evolved from the F1534L (TTC/TTG) mutation. The F1534S (TTC/TCG) mutation has evolved from the F1534S (TTC/TCC) mutation. The most common form of mutation at the F1534 locus found in this study was S1534C, accounting for 20.97%, which may have evolved from the F1534C mutation. In addition, a new non-synonymous mutation M1524I and 28 synonymous mutations were identified in Ae. albopictus populations. Correlation analysis showed that the genetic diversity of Ae. aegypti and Ae. albopictus populations did not correlate with their kdr haplotype diversity (P>0.05), but strong gene flow between populations may have contributed to the evolution of the kdr gene.ConclusionThe study of kdr gene evolution in the two mosquito species may help to identify the evolutionary trend of insecticide resistance at an early stage and provide a theoretical basis for improving the efficiency of biological vector control and subsequent research into new insecticides. |
| first_indexed | 2024-03-11T22:54:45Z |
| format | Article |
| id | doaj.art-8198c1ff9f4a4d2bacfbe50b992c8a58 |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T22:54:45Z |
| publishDate | 2023-09-01 |
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| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-8198c1ff9f4a4d2bacfbe50b992c8a582023-09-21T18:56:03ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-09-011310.3389/fcimb.2023.12658731265873Evolution of knockdown resistance (kdr) mutations of Aedes aegypti and Aedes albopictus in Hainan Island and Leizhou Peninsula, ChinaMinghui Zhao0Minghui Zhao1Xin Ran2Dan Xing3Yun Liao4Wei Liu5Yu Bai6Qiang Zhang7Kan Chen8Lan Liu9Mingyu Wu10Zu Ma11Jian Gao12Hengduan Zhang13Tongyan Zhao14State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, ChinaJiangxi International Travel Healthcare Center, Nanchang, ChinaJiangxi Provincial Center for Disease Control and Prevention, Nanchang, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, ChinaJiangxi International Travel Healthcare Center, Nanchang, ChinaJiangxi International Travel Healthcare Center, Nanchang, ChinaJiangxi International Travel Healthcare Center, Nanchang, ChinaJiangxi International Travel Healthcare Center, Nanchang, ChinaJiangxi International Travel Healthcare Center, Nanchang, ChinaJiangxi International Travel Healthcare Center, Nanchang, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, ChinaJiangsu Provincial Center for Disease Control and Prevention, Nanjing, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, ChinaState Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, ChinaBackgroundAedes aegypti and Aedes albopictus are important vectors of human arboviruses, transmitting arboviral diseases such as yellow fever, dengue, chikungunya and Zika. These two mosquitoes coexist on Hainan Island and the Leizhou Peninsula in China. Over the past 40 years, the distribution of Ae. albopictus has gradually expanded in these areas, while the distribution of Ae. aegypti has declined dramatically mainly due to the ecological changes and some other factors such as heavy use of insecticide indoor based on endophagic bloodfeeding of the species.MethodsThis study focused on the knockdown resistance (kdr) genes of both mosquitoes, investigated their mutations, and analyzed their haplotype and evolutionary diversity combined with population genetic features based on the ND4/ND5 genes to further elucidate the molecular mechanisms underlying the development of insecticide resistance in both mosquitoes.ResultsThree mutations, S989P, V1016G and F1534C, were found to be present in Ae. aegypti populations, and the three mutations occurred synergistically. Multiple mutation types (F1534C/S/L/W) of the F1534 locus are found in Ae. albopictus populations, with the three common mutations F1534C, F1534S and F1534L all having multiple independent origins. The F1534W (TTC/TGG) mutation is thought to have evolved from the F1534L (TTC/TTG) mutation. The F1534S (TTC/TCG) mutation has evolved from the F1534S (TTC/TCC) mutation. The most common form of mutation at the F1534 locus found in this study was S1534C, accounting for 20.97%, which may have evolved from the F1534C mutation. In addition, a new non-synonymous mutation M1524I and 28 synonymous mutations were identified in Ae. albopictus populations. Correlation analysis showed that the genetic diversity of Ae. aegypti and Ae. albopictus populations did not correlate with their kdr haplotype diversity (P>0.05), but strong gene flow between populations may have contributed to the evolution of the kdr gene.ConclusionThe study of kdr gene evolution in the two mosquito species may help to identify the evolutionary trend of insecticide resistance at an early stage and provide a theoretical basis for improving the efficiency of biological vector control and subsequent research into new insecticides.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1265873/fullAe. aegyptiAe. albopictuskdrevolutionpopulation genetics |
| spellingShingle | Minghui Zhao Minghui Zhao Xin Ran Dan Xing Yun Liao Wei Liu Yu Bai Qiang Zhang Kan Chen Lan Liu Mingyu Wu Zu Ma Jian Gao Hengduan Zhang Tongyan Zhao Evolution of knockdown resistance (kdr) mutations of Aedes aegypti and Aedes albopictus in Hainan Island and Leizhou Peninsula, China Frontiers in Cellular and Infection Microbiology Ae. aegypti Ae. albopictus kdr evolution population genetics |
| title | Evolution of knockdown resistance (kdr) mutations of Aedes aegypti and Aedes albopictus in Hainan Island and Leizhou Peninsula, China |
| title_full | Evolution of knockdown resistance (kdr) mutations of Aedes aegypti and Aedes albopictus in Hainan Island and Leizhou Peninsula, China |
| title_fullStr | Evolution of knockdown resistance (kdr) mutations of Aedes aegypti and Aedes albopictus in Hainan Island and Leizhou Peninsula, China |
| title_full_unstemmed | Evolution of knockdown resistance (kdr) mutations of Aedes aegypti and Aedes albopictus in Hainan Island and Leizhou Peninsula, China |
| title_short | Evolution of knockdown resistance (kdr) mutations of Aedes aegypti and Aedes albopictus in Hainan Island and Leizhou Peninsula, China |
| title_sort | evolution of knockdown resistance kdr mutations of aedes aegypti and aedes albopictus in hainan island and leizhou peninsula china |
| topic | Ae. aegypti Ae. albopictus kdr evolution population genetics |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1265873/full |
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