Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves
Acellular nerve allografts (ANGs) represent a promising alternative in nerve repair. Our aim is to improve the structural and biomechanical properties of biocompatible Sondell (SD) and Roosens (RS) based ANGs using genipin (GP) as a crosslinker agent ex vivo. The impact of two concentrations of GP (...
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/2/674 |
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| author | Óscar Darío García-García Marwa El Soury David González-Quevedo David Sánchez-Porras Jesús Chato-Astrain Fernando Campos Víctor Carriel |
| author_facet | Óscar Darío García-García Marwa El Soury David González-Quevedo David Sánchez-Porras Jesús Chato-Astrain Fernando Campos Víctor Carriel |
| author_sort | Óscar Darío García-García |
| collection | DOAJ |
| description | Acellular nerve allografts (ANGs) represent a promising alternative in nerve repair. Our aim is to improve the structural and biomechanical properties of biocompatible Sondell (SD) and Roosens (RS) based ANGs using genipin (GP) as a crosslinker agent ex vivo. The impact of two concentrations of GP (0.10% and 0.25%) on Wistar rat sciatic nerve-derived ANGs was assessed at the histological, biomechanical, and biocompatibility levels. Histology confirmed the differences between SD and RS procedures, but not remarkable changes were induced by GP, which helped to preserve the nerve histological pattern. Tensile test revealed that GP enhanced the biomechanical properties of SD and RS ANGs, being the crosslinked RS ANGs more comparable to the native nerves used as control. The evaluation of the ANGs biocompatibility conducted with adipose-derived mesenchymal stem cells cultured within the ANGs confirmed a high degree of biocompatibility in all ANGs, especially in RS and RS-GP 0.10% ANGs. Finally, this study demonstrates that the use of GP could be an efficient alternative to improve the biomechanical properties of ANGs with a slight impact on the biocompatibility and histological pattern. For these reasons, we hypothesize that our novel crosslinked ANGs could be a suitable alternative for future in vivo preclinical studies. |
| first_indexed | 2024-03-09T05:08:10Z |
| format | Article |
| id | doaj.art-819a8be559af49fb8c20d3b5677a2078 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:08:10Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-819a8be559af49fb8c20d3b5677a20782023-12-03T12:52:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-0122267410.3390/ijms22020674Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral NervesÓscar Darío García-García0Marwa El Soury1David González-Quevedo2David Sánchez-Porras3Jesús Chato-Astrain4Fernando Campos5Víctor Carriel6Tissue Engineering Group, Department of Histology, University of Granada, 18016 Granada, SpainTissue Engineering Group, Department of Histology, University of Granada, 18016 Granada, SpainTissue Engineering Group, Department of Histology, University of Granada, 18016 Granada, SpainTissue Engineering Group, Department of Histology, University of Granada, 18016 Granada, SpainTissue Engineering Group, Department of Histology, University of Granada, 18016 Granada, SpainTissue Engineering Group, Department of Histology, University of Granada, 18016 Granada, SpainTissue Engineering Group, Department of Histology, University of Granada, 18016 Granada, SpainAcellular nerve allografts (ANGs) represent a promising alternative in nerve repair. Our aim is to improve the structural and biomechanical properties of biocompatible Sondell (SD) and Roosens (RS) based ANGs using genipin (GP) as a crosslinker agent ex vivo. The impact of two concentrations of GP (0.10% and 0.25%) on Wistar rat sciatic nerve-derived ANGs was assessed at the histological, biomechanical, and biocompatibility levels. Histology confirmed the differences between SD and RS procedures, but not remarkable changes were induced by GP, which helped to preserve the nerve histological pattern. Tensile test revealed that GP enhanced the biomechanical properties of SD and RS ANGs, being the crosslinked RS ANGs more comparable to the native nerves used as control. The evaluation of the ANGs biocompatibility conducted with adipose-derived mesenchymal stem cells cultured within the ANGs confirmed a high degree of biocompatibility in all ANGs, especially in RS and RS-GP 0.10% ANGs. Finally, this study demonstrates that the use of GP could be an efficient alternative to improve the biomechanical properties of ANGs with a slight impact on the biocompatibility and histological pattern. For these reasons, we hypothesize that our novel crosslinked ANGs could be a suitable alternative for future in vivo preclinical studies.https://www.mdpi.com/1422-0067/22/2/674tissue engineeringnerve repairnerve tissue decellularizationgenipinchemical crosslinkinghistology |
| spellingShingle | Óscar Darío García-García Marwa El Soury David González-Quevedo David Sánchez-Porras Jesús Chato-Astrain Fernando Campos Víctor Carriel Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves International Journal of Molecular Sciences tissue engineering nerve repair nerve tissue decellularization genipin chemical crosslinking histology |
| title | Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves |
| title_full | Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves |
| title_fullStr | Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves |
| title_full_unstemmed | Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves |
| title_short | Histological, Biomechanical, and Biological Properties of Genipin-Crosslinked Decellularized Peripheral Nerves |
| title_sort | histological biomechanical and biological properties of genipin crosslinked decellularized peripheral nerves |
| topic | tissue engineering nerve repair nerve tissue decellularization genipin chemical crosslinking histology |
| url | https://www.mdpi.com/1422-0067/22/2/674 |
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