Comparative Transcriptomic Analyses Revealed the Effects of Poly (I:C) on the Liver and Spleen of <i>Argyrosomus japonicus</i>
Poly (I:C) can work as an immunostimulant and a viral vaccine; however, its functional mechanism in aquatic animals needs to be further investigated. In this study, comparative transcriptomic analyses were performed to investigate the effects of poly (I:C) on <i>Argyrosomus japonicus</i>...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/17/9801 |
| Summary: | Poly (I:C) can work as an immunostimulant and a viral vaccine; however, its functional mechanism in aquatic animals needs to be further investigated. In this study, comparative transcriptomic analyses were performed to investigate the effects of poly (I:C) on <i>Argyrosomus japonicus</i> at 12 h and 48 h postinjection. A total of 194 and 294 differentially expressed genes were obtained in the liver and spleen, respectively. At 12 h, poly (I:C) injection could significantly influence the function of the metabolism-related pathways and immune-related pathways in the liver through the upregulation of the genes <i>GST</i>, <i>LPIN</i>, <i>FOXO1</i>, <i>CYP24A1</i>, <i>ECM1,</i> and <i>SGK1</i>, and the downregulation of the genes <i>IL-1β</i>, <i>CXC19</i>, <i>TNFAIP3</i>, and <i>IRF1</i>. At 48 h, poly (I:C) could enhance the liver energy metabolism by upregulating the genes <i>TXNRD</i> and <i>ECM1</i>, while it also induced some injury in the cells with the downregulation of the genes <i>HBA</i> and <i>CYP24A1</i>. In the spleen, poly (I:C) could regulate the fish immunity and inflammatory response by upregulating the genes <i>DDIT4</i>, <i>C3</i>, <i>EFNA</i>, and <i>MNK</i>, and by downregulating the genes <i>ABCA1</i>, <i>SORT1</i>, <i>TNF</i>, <i>TLR2</i>, <i>IL8</i>, and <i>MHCII</i> at 12 h, and at 48 h, the poly (I:C) had a similar influence as that in the liver. Intersection analyses demonstrated that <i>CYP24A1</i> and <i>ECM1</i> were the main functional genes that contributed to the health of the liver. Ten and four genes participated in maintaining the health of the two tissues after 12 h and 48 h, respectively. In summary, our results provided a new insight into ploy (I:C) application in <i>A. japonicus</i>, and it also helped us to better understand the fish response mechanism to the viral vaccine injection. |
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| ISSN: | 1661-6596 1422-0067 |