Comparative Transcriptomic Analyses Revealed the Effects of Poly (I:C) on the Liver and Spleen of <i>Argyrosomus japonicus</i>
Poly (I:C) can work as an immunostimulant and a viral vaccine; however, its functional mechanism in aquatic animals needs to be further investigated. In this study, comparative transcriptomic analyses were performed to investigate the effects of poly (I:C) on <i>Argyrosomus japonicus</i>...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/17/9801 |
| _version_ | 1797495149322829824 |
|---|---|
| author | Anle Xu Fei Han Yuan Zhang Tao Zhou Tianxiang Gao |
| author_facet | Anle Xu Fei Han Yuan Zhang Tao Zhou Tianxiang Gao |
| author_sort | Anle Xu |
| collection | DOAJ |
| description | Poly (I:C) can work as an immunostimulant and a viral vaccine; however, its functional mechanism in aquatic animals needs to be further investigated. In this study, comparative transcriptomic analyses were performed to investigate the effects of poly (I:C) on <i>Argyrosomus japonicus</i> at 12 h and 48 h postinjection. A total of 194 and 294 differentially expressed genes were obtained in the liver and spleen, respectively. At 12 h, poly (I:C) injection could significantly influence the function of the metabolism-related pathways and immune-related pathways in the liver through the upregulation of the genes <i>GST</i>, <i>LPIN</i>, <i>FOXO1</i>, <i>CYP24A1</i>, <i>ECM1,</i> and <i>SGK1</i>, and the downregulation of the genes <i>IL-1β</i>, <i>CXC19</i>, <i>TNFAIP3</i>, and <i>IRF1</i>. At 48 h, poly (I:C) could enhance the liver energy metabolism by upregulating the genes <i>TXNRD</i> and <i>ECM1</i>, while it also induced some injury in the cells with the downregulation of the genes <i>HBA</i> and <i>CYP24A1</i>. In the spleen, poly (I:C) could regulate the fish immunity and inflammatory response by upregulating the genes <i>DDIT4</i>, <i>C3</i>, <i>EFNA</i>, and <i>MNK</i>, and by downregulating the genes <i>ABCA1</i>, <i>SORT1</i>, <i>TNF</i>, <i>TLR2</i>, <i>IL8</i>, and <i>MHCII</i> at 12 h, and at 48 h, the poly (I:C) had a similar influence as that in the liver. Intersection analyses demonstrated that <i>CYP24A1</i> and <i>ECM1</i> were the main functional genes that contributed to the health of the liver. Ten and four genes participated in maintaining the health of the two tissues after 12 h and 48 h, respectively. In summary, our results provided a new insight into ploy (I:C) application in <i>A. japonicus</i>, and it also helped us to better understand the fish response mechanism to the viral vaccine injection. |
| first_indexed | 2024-03-10T01:44:19Z |
| format | Article |
| id | doaj.art-81a193bdc0684cc1bc1fc40896a5548b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:44:19Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-81a193bdc0684cc1bc1fc40896a5548b2023-11-23T13:17:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012317980110.3390/ijms23179801Comparative Transcriptomic Analyses Revealed the Effects of Poly (I:C) on the Liver and Spleen of <i>Argyrosomus japonicus</i>Anle Xu0Fei Han1Yuan Zhang2Tao Zhou3Tianxiang Gao4Fisheries College, Zhejiang Ocean University, Zhoushan 316022, ChinaFisheries College, Ocean University of China, Qingdao 266003, ChinaFisheries College, Ocean University of China, Qingdao 266003, ChinaCollege of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, ChinaFisheries College, Zhejiang Ocean University, Zhoushan 316022, ChinaPoly (I:C) can work as an immunostimulant and a viral vaccine; however, its functional mechanism in aquatic animals needs to be further investigated. In this study, comparative transcriptomic analyses were performed to investigate the effects of poly (I:C) on <i>Argyrosomus japonicus</i> at 12 h and 48 h postinjection. A total of 194 and 294 differentially expressed genes were obtained in the liver and spleen, respectively. At 12 h, poly (I:C) injection could significantly influence the function of the metabolism-related pathways and immune-related pathways in the liver through the upregulation of the genes <i>GST</i>, <i>LPIN</i>, <i>FOXO1</i>, <i>CYP24A1</i>, <i>ECM1,</i> and <i>SGK1</i>, and the downregulation of the genes <i>IL-1β</i>, <i>CXC19</i>, <i>TNFAIP3</i>, and <i>IRF1</i>. At 48 h, poly (I:C) could enhance the liver energy metabolism by upregulating the genes <i>TXNRD</i> and <i>ECM1</i>, while it also induced some injury in the cells with the downregulation of the genes <i>HBA</i> and <i>CYP24A1</i>. In the spleen, poly (I:C) could regulate the fish immunity and inflammatory response by upregulating the genes <i>DDIT4</i>, <i>C3</i>, <i>EFNA</i>, and <i>MNK</i>, and by downregulating the genes <i>ABCA1</i>, <i>SORT1</i>, <i>TNF</i>, <i>TLR2</i>, <i>IL8</i>, and <i>MHCII</i> at 12 h, and at 48 h, the poly (I:C) had a similar influence as that in the liver. Intersection analyses demonstrated that <i>CYP24A1</i> and <i>ECM1</i> were the main functional genes that contributed to the health of the liver. Ten and four genes participated in maintaining the health of the two tissues after 12 h and 48 h, respectively. In summary, our results provided a new insight into ploy (I:C) application in <i>A. japonicus</i>, and it also helped us to better understand the fish response mechanism to the viral vaccine injection.https://www.mdpi.com/1422-0067/23/17/9801<i>Argyrosomus japonicus</i>poly (I:C)comparative transcriptomic analysesfunctional genesimmunity |
| spellingShingle | Anle Xu Fei Han Yuan Zhang Tao Zhou Tianxiang Gao Comparative Transcriptomic Analyses Revealed the Effects of Poly (I:C) on the Liver and Spleen of <i>Argyrosomus japonicus</i> International Journal of Molecular Sciences <i>Argyrosomus japonicus</i> poly (I:C) comparative transcriptomic analyses functional genes immunity |
| title | Comparative Transcriptomic Analyses Revealed the Effects of Poly (I:C) on the Liver and Spleen of <i>Argyrosomus japonicus</i> |
| title_full | Comparative Transcriptomic Analyses Revealed the Effects of Poly (I:C) on the Liver and Spleen of <i>Argyrosomus japonicus</i> |
| title_fullStr | Comparative Transcriptomic Analyses Revealed the Effects of Poly (I:C) on the Liver and Spleen of <i>Argyrosomus japonicus</i> |
| title_full_unstemmed | Comparative Transcriptomic Analyses Revealed the Effects of Poly (I:C) on the Liver and Spleen of <i>Argyrosomus japonicus</i> |
| title_short | Comparative Transcriptomic Analyses Revealed the Effects of Poly (I:C) on the Liver and Spleen of <i>Argyrosomus japonicus</i> |
| title_sort | comparative transcriptomic analyses revealed the effects of poly i c on the liver and spleen of i argyrosomus japonicus i |
| topic | <i>Argyrosomus japonicus</i> poly (I:C) comparative transcriptomic analyses functional genes immunity |
| url | https://www.mdpi.com/1422-0067/23/17/9801 |
| work_keys_str_mv | AT anlexu comparativetranscriptomicanalysesrevealedtheeffectsofpolyicontheliverandspleenofiargyrosomusjaponicusi AT feihan comparativetranscriptomicanalysesrevealedtheeffectsofpolyicontheliverandspleenofiargyrosomusjaponicusi AT yuanzhang comparativetranscriptomicanalysesrevealedtheeffectsofpolyicontheliverandspleenofiargyrosomusjaponicusi AT taozhou comparativetranscriptomicanalysesrevealedtheeffectsofpolyicontheliverandspleenofiargyrosomusjaponicusi AT tianxianggao comparativetranscriptomicanalysesrevealedtheeffectsofpolyicontheliverandspleenofiargyrosomusjaponicusi |