Prevalence and association of Epstein-Barr virus infection with sinonasal inverted papilloma and sinonasal squamous cell carcinoma in the northeastern Thai population
Abstract Aims Sinonasal inverted papillomas (SIP) and sinonasal squamous cell carcinomas (SNSCC) are sinonasal tumors with unclear etiology and pathogenesis. Epstein-Barr virus (EBV) has been detected in these tumors but information concerning their association is still limited. This study aimed to...
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| Format: | Article |
| Language: | English |
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BMC
2020-06-01
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| Series: | Infectious Agents and Cancer |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13027-020-00308-5 |
| _version_ | 1819071977449586688 |
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| author | Thawaree Nukpook Tipaya Ekalaksananan Watchareporn Teeramatwanich Natcha Patarapadungkit Surachat Chaiwiriyakul Patravoot Vatanasapt Sirinart Aromseree Chamsai Pientong |
| author_facet | Thawaree Nukpook Tipaya Ekalaksananan Watchareporn Teeramatwanich Natcha Patarapadungkit Surachat Chaiwiriyakul Patravoot Vatanasapt Sirinart Aromseree Chamsai Pientong |
| author_sort | Thawaree Nukpook |
| collection | DOAJ |
| description | Abstract Aims Sinonasal inverted papillomas (SIP) and sinonasal squamous cell carcinomas (SNSCC) are sinonasal tumors with unclear etiology and pathogenesis. Epstein-Barr virus (EBV) has been detected in these tumors but information concerning their association is still limited. This study aimed to investigate the prevalence in, and association of EBV infection with SIP and SNSCC in northeastern Thailand. Methods DNA was extracted from 226 formalin-fixed, paraffin-embedded tissues including 80 nasal polyps (NP; the control group), 64 SIP and 82 SNSCC samples. Presence of EBV in these tissues was investigated using real-time PCR and their localization within tissues was confirmed using in situ hybridization (ISH). Characteristics of patients and the association of EBV prevalence with sinonasal tumors were analyzed. Results SIP and SNSCC were frequently found in people aged > 50 years and more often in males than in females (3:1 ratio). EBV infection was detected in 33.75, 64.06 and 37.80% of NP, SIP and SNSCC tissues, respectively, by real-time PCR. There was a statistically significant association between EBV infection and SIP (odds ratio [OR] = 3.52). This was not the case for SNSCC when compared to the NP group (OR = 1.83). Interestingly, EBV infection tended to be associated with inflammation and dysplasia in SIP. In SNSCC, EBV was mostly found in samples with undifferentiated or poorly differentiated cell types as well as in recurrent cases and lymph-node metastasis. Using ISH, EBV was detected only in infiltrating lymphocytes within the tumor stroma, not in the tumor epithelial cells. Conclusions Infiltrating lymphocytes containing EBV in the tumor microenvironment might enhance tumorigenesis of SIP and SNSCC. The mechanism by which EBV promotes development of SIP and SNSCC needs to be elucidated in the future. |
| first_indexed | 2024-12-21T17:30:24Z |
| format | Article |
| id | doaj.art-81a4ba9ff0c6453e8c4127548f74b287 |
| institution | Directory Open Access Journal |
| issn | 1750-9378 |
| language | English |
| last_indexed | 2024-12-21T17:30:24Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Infectious Agents and Cancer |
| spelling | doaj.art-81a4ba9ff0c6453e8c4127548f74b2872022-12-21T18:55:57ZengBMCInfectious Agents and Cancer1750-93782020-06-0115111010.1186/s13027-020-00308-5Prevalence and association of Epstein-Barr virus infection with sinonasal inverted papilloma and sinonasal squamous cell carcinoma in the northeastern Thai populationThawaree Nukpook0Tipaya Ekalaksananan1Watchareporn Teeramatwanich2Natcha Patarapadungkit3Surachat Chaiwiriyakul4Patravoot Vatanasapt5Sirinart Aromseree6Chamsai Pientong7Department of Microbiology, Faculty of Medicine, Khon Kaen UniversityDepartment of Microbiology, Faculty of Medicine, Khon Kaen UniversityHPV & EBV and Carcinogenesis Research Group, Khon Kaen UniversityHPV & EBV and Carcinogenesis Research Group, Khon Kaen UniversityDepartment of Pathology, Faculty of Medicine, Khon Kaen UniversityHPV & EBV and Carcinogenesis Research Group, Khon Kaen UniversityDepartment of Microbiology, Faculty of Medicine, Khon Kaen UniversityDepartment of Microbiology, Faculty of Medicine, Khon Kaen UniversityAbstract Aims Sinonasal inverted papillomas (SIP) and sinonasal squamous cell carcinomas (SNSCC) are sinonasal tumors with unclear etiology and pathogenesis. Epstein-Barr virus (EBV) has been detected in these tumors but information concerning their association is still limited. This study aimed to investigate the prevalence in, and association of EBV infection with SIP and SNSCC in northeastern Thailand. Methods DNA was extracted from 226 formalin-fixed, paraffin-embedded tissues including 80 nasal polyps (NP; the control group), 64 SIP and 82 SNSCC samples. Presence of EBV in these tissues was investigated using real-time PCR and their localization within tissues was confirmed using in situ hybridization (ISH). Characteristics of patients and the association of EBV prevalence with sinonasal tumors were analyzed. Results SIP and SNSCC were frequently found in people aged > 50 years and more often in males than in females (3:1 ratio). EBV infection was detected in 33.75, 64.06 and 37.80% of NP, SIP and SNSCC tissues, respectively, by real-time PCR. There was a statistically significant association between EBV infection and SIP (odds ratio [OR] = 3.52). This was not the case for SNSCC when compared to the NP group (OR = 1.83). Interestingly, EBV infection tended to be associated with inflammation and dysplasia in SIP. In SNSCC, EBV was mostly found in samples with undifferentiated or poorly differentiated cell types as well as in recurrent cases and lymph-node metastasis. Using ISH, EBV was detected only in infiltrating lymphocytes within the tumor stroma, not in the tumor epithelial cells. Conclusions Infiltrating lymphocytes containing EBV in the tumor microenvironment might enhance tumorigenesis of SIP and SNSCC. The mechanism by which EBV promotes development of SIP and SNSCC needs to be elucidated in the future.http://link.springer.com/article/10.1186/s13027-020-00308-5Epstein-Barr virusSinonasal inverted papillomaSinonasal squamous cell carcinomaInflammation |
| spellingShingle | Thawaree Nukpook Tipaya Ekalaksananan Watchareporn Teeramatwanich Natcha Patarapadungkit Surachat Chaiwiriyakul Patravoot Vatanasapt Sirinart Aromseree Chamsai Pientong Prevalence and association of Epstein-Barr virus infection with sinonasal inverted papilloma and sinonasal squamous cell carcinoma in the northeastern Thai population Infectious Agents and Cancer Epstein-Barr virus Sinonasal inverted papilloma Sinonasal squamous cell carcinoma Inflammation |
| title | Prevalence and association of Epstein-Barr virus infection with sinonasal inverted papilloma and sinonasal squamous cell carcinoma in the northeastern Thai population |
| title_full | Prevalence and association of Epstein-Barr virus infection with sinonasal inverted papilloma and sinonasal squamous cell carcinoma in the northeastern Thai population |
| title_fullStr | Prevalence and association of Epstein-Barr virus infection with sinonasal inverted papilloma and sinonasal squamous cell carcinoma in the northeastern Thai population |
| title_full_unstemmed | Prevalence and association of Epstein-Barr virus infection with sinonasal inverted papilloma and sinonasal squamous cell carcinoma in the northeastern Thai population |
| title_short | Prevalence and association of Epstein-Barr virus infection with sinonasal inverted papilloma and sinonasal squamous cell carcinoma in the northeastern Thai population |
| title_sort | prevalence and association of epstein barr virus infection with sinonasal inverted papilloma and sinonasal squamous cell carcinoma in the northeastern thai population |
| topic | Epstein-Barr virus Sinonasal inverted papilloma Sinonasal squamous cell carcinoma Inflammation |
| url | http://link.springer.com/article/10.1186/s13027-020-00308-5 |
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