Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production
Summary: Recent technical advances in cell-free protein synthesis (CFPS) offer several advantages over cell-based expression systems, including the application of cellular machinery, such as transcription and translation, in the test tube. Inspired by the advantages of CFPS, we have fabricated a mul...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223011665 |
| _version_ | 1797774555250425856 |
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| author | Hyangsu Nam Taehyeon Kim Sunghyun Moon Yoonbin Ji Jong Bum Lee |
| author_facet | Hyangsu Nam Taehyeon Kim Sunghyun Moon Yoonbin Ji Jong Bum Lee |
| author_sort | Hyangsu Nam |
| collection | DOAJ |
| description | Summary: Recent technical advances in cell-free protein synthesis (CFPS) offer several advantages over cell-based expression systems, including the application of cellular machinery, such as transcription and translation, in the test tube. Inspired by the advantages of CFPS, we have fabricated a multimeric genomic DNA hydrogel (mGD-gel) via rolling circle chain amplification (RCCA) using dual single-stranded circular plasmids with multiple primers. The mGD-gel exhibited significantly enhanced protein yield. In addition, mGD-gel can be reused at least five times, and the shape of the mGD-gel can be easily manipulated without losing the feasibility of protein expression. The mGD-gel platform based on the self-assembly of multimeric genomic DNA strands (mGD strands) has the potential to be used in CFPS systems for a variety of biotechnological applications. |
| first_indexed | 2024-03-12T22:22:45Z |
| format | Article |
| id | doaj.art-81a92ce1975d4f3b9baf57719c13cc39 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-12T22:22:45Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-81a92ce1975d4f3b9baf57719c13cc392023-07-23T04:55:30ZengElsevieriScience2589-00422023-07-01267107089Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein productionHyangsu Nam0Taehyeon Kim1Sunghyun Moon2Yoonbin Ji3Jong Bum Lee4Department of Chemical Engineering, University of Seoul, 163 Seoulsiripdaero, Dongdaemun-gu, Seoul 02504, Republic of KoreaDepartment of Chemical Engineering, University of Seoul, 163 Seoulsiripdaero, Dongdaemun-gu, Seoul 02504, Republic of KoreaDepartment of Chemical Engineering, University of Seoul, 163 Seoulsiripdaero, Dongdaemun-gu, Seoul 02504, Republic of KoreaDepartment of Chemical Engineering, University of Seoul, 163 Seoulsiripdaero, Dongdaemun-gu, Seoul 02504, Republic of KoreaDepartment of Chemical Engineering, University of Seoul, 163 Seoulsiripdaero, Dongdaemun-gu, Seoul 02504, Republic of Korea; Corresponding authorSummary: Recent technical advances in cell-free protein synthesis (CFPS) offer several advantages over cell-based expression systems, including the application of cellular machinery, such as transcription and translation, in the test tube. Inspired by the advantages of CFPS, we have fabricated a multimeric genomic DNA hydrogel (mGD-gel) via rolling circle chain amplification (RCCA) using dual single-stranded circular plasmids with multiple primers. The mGD-gel exhibited significantly enhanced protein yield. In addition, mGD-gel can be reused at least five times, and the shape of the mGD-gel can be easily manipulated without losing the feasibility of protein expression. The mGD-gel platform based on the self-assembly of multimeric genomic DNA strands (mGD strands) has the potential to be used in CFPS systems for a variety of biotechnological applications.http://www.sciencedirect.com/science/article/pii/S2589004223011665Scaffolds in supramolecular chemistryMolecular self-assemblyBiotechnology |
| spellingShingle | Hyangsu Nam Taehyeon Kim Sunghyun Moon Yoonbin Ji Jong Bum Lee Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production iScience Scaffolds in supramolecular chemistry Molecular self-assembly Biotechnology |
| title | Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production |
| title_full | Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production |
| title_fullStr | Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production |
| title_full_unstemmed | Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production |
| title_short | Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production |
| title_sort | self assembly of a multimeric genomic hydrogel via multi primed chain reaction of dual single stranded circular plasmids for cell free protein production |
| topic | Scaffolds in supramolecular chemistry Molecular self-assembly Biotechnology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223011665 |
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