Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production
Summary: Recent technical advances in cell-free protein synthesis (CFPS) offer several advantages over cell-based expression systems, including the application of cellular machinery, such as transcription and translation, in the test tube. Inspired by the advantages of CFPS, we have fabricated a mul...
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Format: | Article |
Language: | English |
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Elsevier
2023-07-01
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Series: | iScience |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223011665 |
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author | Hyangsu Nam Taehyeon Kim Sunghyun Moon Yoonbin Ji Jong Bum Lee |
author_facet | Hyangsu Nam Taehyeon Kim Sunghyun Moon Yoonbin Ji Jong Bum Lee |
author_sort | Hyangsu Nam |
collection | DOAJ |
description | Summary: Recent technical advances in cell-free protein synthesis (CFPS) offer several advantages over cell-based expression systems, including the application of cellular machinery, such as transcription and translation, in the test tube. Inspired by the advantages of CFPS, we have fabricated a multimeric genomic DNA hydrogel (mGD-gel) via rolling circle chain amplification (RCCA) using dual single-stranded circular plasmids with multiple primers. The mGD-gel exhibited significantly enhanced protein yield. In addition, mGD-gel can be reused at least five times, and the shape of the mGD-gel can be easily manipulated without losing the feasibility of protein expression. The mGD-gel platform based on the self-assembly of multimeric genomic DNA strands (mGD strands) has the potential to be used in CFPS systems for a variety of biotechnological applications. |
first_indexed | 2024-03-12T22:22:45Z |
format | Article |
id | doaj.art-81a92ce1975d4f3b9baf57719c13cc39 |
institution | Directory Open Access Journal |
issn | 2589-0042 |
language | English |
last_indexed | 2024-03-12T22:22:45Z |
publishDate | 2023-07-01 |
publisher | Elsevier |
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series | iScience |
spelling | doaj.art-81a92ce1975d4f3b9baf57719c13cc392023-07-23T04:55:30ZengElsevieriScience2589-00422023-07-01267107089Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein productionHyangsu Nam0Taehyeon Kim1Sunghyun Moon2Yoonbin Ji3Jong Bum Lee4Department of Chemical Engineering, University of Seoul, 163 Seoulsiripdaero, Dongdaemun-gu, Seoul 02504, Republic of KoreaDepartment of Chemical Engineering, University of Seoul, 163 Seoulsiripdaero, Dongdaemun-gu, Seoul 02504, Republic of KoreaDepartment of Chemical Engineering, University of Seoul, 163 Seoulsiripdaero, Dongdaemun-gu, Seoul 02504, Republic of KoreaDepartment of Chemical Engineering, University of Seoul, 163 Seoulsiripdaero, Dongdaemun-gu, Seoul 02504, Republic of KoreaDepartment of Chemical Engineering, University of Seoul, 163 Seoulsiripdaero, Dongdaemun-gu, Seoul 02504, Republic of Korea; Corresponding authorSummary: Recent technical advances in cell-free protein synthesis (CFPS) offer several advantages over cell-based expression systems, including the application of cellular machinery, such as transcription and translation, in the test tube. Inspired by the advantages of CFPS, we have fabricated a multimeric genomic DNA hydrogel (mGD-gel) via rolling circle chain amplification (RCCA) using dual single-stranded circular plasmids with multiple primers. The mGD-gel exhibited significantly enhanced protein yield. In addition, mGD-gel can be reused at least five times, and the shape of the mGD-gel can be easily manipulated without losing the feasibility of protein expression. The mGD-gel platform based on the self-assembly of multimeric genomic DNA strands (mGD strands) has the potential to be used in CFPS systems for a variety of biotechnological applications.http://www.sciencedirect.com/science/article/pii/S2589004223011665Scaffolds in supramolecular chemistryMolecular self-assemblyBiotechnology |
spellingShingle | Hyangsu Nam Taehyeon Kim Sunghyun Moon Yoonbin Ji Jong Bum Lee Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production iScience Scaffolds in supramolecular chemistry Molecular self-assembly Biotechnology |
title | Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production |
title_full | Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production |
title_fullStr | Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production |
title_full_unstemmed | Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production |
title_short | Self-assembly of a multimeric genomic hydrogel via multi-primed chain reaction of dual single-stranded circular plasmids for cell-free protein production |
title_sort | self assembly of a multimeric genomic hydrogel via multi primed chain reaction of dual single stranded circular plasmids for cell free protein production |
topic | Scaffolds in supramolecular chemistry Molecular self-assembly Biotechnology |
url | http://www.sciencedirect.com/science/article/pii/S2589004223011665 |
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