The Role of Thyroid Transcription Factor-1 DNA Binding Activity in Lung Cancer
Background and objective TTF-1 is a homeodomain transcriptional regulator of both structural organization of the lung and differentiation of highly specialized epithelial cell types such as alveolar typeⅡcells. This study is to investigate the Thyroid transcription factor-1 (TTF-1) DNA binding activ...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2009-02-01
|
| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://www.lungca.org/index.php?journal=01&page=article&op=view&path%5B%5D=10.3779%2Fj.issn.1009-3419.2009.02.06&path%5B%5D=226 |
| _version_ | 1818607056295297024 |
|---|---|
| author | Zongwei GAO Chunbao GUO Xianqing JIN |
| author_facet | Zongwei GAO Chunbao GUO Xianqing JIN |
| author_sort | Zongwei GAO |
| collection | DOAJ |
| description | Background and objective TTF-1 is a homeodomain transcriptional regulator of both structural organization of the lung and differentiation of highly specialized epithelial cell types such as alveolar typeⅡcells. This study is to investigate the Thyroid transcription factor-1 (TTF-1) DNA binding activity in lung cancer tissue and its significance with lung cancer pathologic type and differentiation level. Methods The TTF-1 DNA binding activity wasdetected in lung cancer tissue by Electrophoretic mobility shift assay (EMSA), autoradiography and photo densitometry. Results The optical density of TTF-1 DNA binding activity in adenocarcinoma tissue was 172±23.2 and it was remarkably higher than that in other pathological types, including 141±16.3 of small cell carcinomas and 122±13.6 of squamous carcinoma (P <0.05). The optical density of TTF-1 DNA binding activity in highly differentiated lung cancer tissues was significantly higher than that in poor-differentiated lung cancer (P <0.05). Overall survival and disease-free survival rates were better among patients with low TTF-1 DNA binding activity levels than those in patients with high levels. Conclusion TTF-1 DNA binding activity is a potential predictor of lung cancer metastasis and survival. |
| first_indexed | 2024-12-16T14:20:41Z |
| format | Article |
| id | doaj.art-81ac03047d444b499822d59bd07e2f03 |
| institution | Directory Open Access Journal |
| issn | 1009-3419 1999-6187 |
| language | zho |
| last_indexed | 2024-12-16T14:20:41Z |
| publishDate | 2009-02-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-81ac03047d444b499822d59bd07e2f032022-12-21T22:28:30ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872009-02-01122127130The Role of Thyroid Transcription Factor-1 DNA Binding Activity in Lung CancerZongwei GAOChunbao GUOXianqing JINBackground and objective TTF-1 is a homeodomain transcriptional regulator of both structural organization of the lung and differentiation of highly specialized epithelial cell types such as alveolar typeⅡcells. This study is to investigate the Thyroid transcription factor-1 (TTF-1) DNA binding activity in lung cancer tissue and its significance with lung cancer pathologic type and differentiation level. Methods The TTF-1 DNA binding activity wasdetected in lung cancer tissue by Electrophoretic mobility shift assay (EMSA), autoradiography and photo densitometry. Results The optical density of TTF-1 DNA binding activity in adenocarcinoma tissue was 172±23.2 and it was remarkably higher than that in other pathological types, including 141±16.3 of small cell carcinomas and 122±13.6 of squamous carcinoma (P <0.05). The optical density of TTF-1 DNA binding activity in highly differentiated lung cancer tissues was significantly higher than that in poor-differentiated lung cancer (P <0.05). Overall survival and disease-free survival rates were better among patients with low TTF-1 DNA binding activity levels than those in patients with high levels. Conclusion TTF-1 DNA binding activity is a potential predictor of lung cancer metastasis and survival.http://www.lungca.org/index.php?journal=01&page=article&op=view&path%5B%5D=10.3779%2Fj.issn.1009-3419.2009.02.06&path%5B%5D=226Human TTF1 proteinLung neoplasmsElectrophoretic mobility shift assayAutoradiography |
| spellingShingle | Zongwei GAO Chunbao GUO Xianqing JIN The Role of Thyroid Transcription Factor-1 DNA Binding Activity in Lung Cancer Chinese Journal of Lung Cancer Human TTF1 protein Lung neoplasms Electrophoretic mobility shift assay Autoradiography |
| title | The Role of Thyroid Transcription Factor-1 DNA Binding Activity in Lung Cancer |
| title_full | The Role of Thyroid Transcription Factor-1 DNA Binding Activity in Lung Cancer |
| title_fullStr | The Role of Thyroid Transcription Factor-1 DNA Binding Activity in Lung Cancer |
| title_full_unstemmed | The Role of Thyroid Transcription Factor-1 DNA Binding Activity in Lung Cancer |
| title_short | The Role of Thyroid Transcription Factor-1 DNA Binding Activity in Lung Cancer |
| title_sort | role of thyroid transcription factor 1 dna binding activity in lung cancer |
| topic | Human TTF1 protein Lung neoplasms Electrophoretic mobility shift assay Autoradiography |
| url | http://www.lungca.org/index.php?journal=01&page=article&op=view&path%5B%5D=10.3779%2Fj.issn.1009-3419.2009.02.06&path%5B%5D=226 |
| work_keys_str_mv | AT zongweigao theroleofthyroidtranscriptionfactor1dnabindingactivityinlungcancer AT chunbaoguo theroleofthyroidtranscriptionfactor1dnabindingactivityinlungcancer AT xianqingjin theroleofthyroidtranscriptionfactor1dnabindingactivityinlungcancer AT zongweigao roleofthyroidtranscriptionfactor1dnabindingactivityinlungcancer AT chunbaoguo roleofthyroidtranscriptionfactor1dnabindingactivityinlungcancer AT xianqingjin roleofthyroidtranscriptionfactor1dnabindingactivityinlungcancer |