Inhibition of Period Gene Expression Causes Repression of Cell Cycle Progression and Cell Growth in the Bombyx mori Cells
Circadian clock system disorders can lead to uncontrolled cell proliferation, but the molecular mechanism remains unknown. We used a Bombyx mori animal model of single Period gene (BmPer) expression to investigate this mechanism. A slow growing developmental cell model (Per-KD) was isolated from a B...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2019.00537/full |
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| author | Jian-Feng Qiu Jian-Feng Qiu Xue Li Xue Li Wen-Zhao Cui Wen-Zhao Cui Xiao-Fei Liu Xiao-Fei Liu Hui Tao Hui Tao Kun Yang Kun Yang Tai-Ming Dai Tai-Ming Dai Yang-Hu Sima Yang-Hu Sima Shi-Qing Xu Shi-Qing Xu |
| author_facet | Jian-Feng Qiu Jian-Feng Qiu Xue Li Xue Li Wen-Zhao Cui Wen-Zhao Cui Xiao-Fei Liu Xiao-Fei Liu Hui Tao Hui Tao Kun Yang Kun Yang Tai-Ming Dai Tai-Ming Dai Yang-Hu Sima Yang-Hu Sima Shi-Qing Xu Shi-Qing Xu |
| author_sort | Jian-Feng Qiu |
| collection | DOAJ |
| description | Circadian clock system disorders can lead to uncontrolled cell proliferation, but the molecular mechanism remains unknown. We used a Bombyx mori animal model of single Period gene (BmPer) expression to investigate this mechanism. A slow growing developmental cell model (Per-KD) was isolated from a B. mori ovarian cell line (BmN) by continuous knock down of BmPer expression. The effects of BmPer expression knockdown (Per-KD) on cell proliferation and apoptosis were opposite to those of m/hPer1 and m/hPer2 in mammals. The knockdown of BmPer expression led to cell cycle deceleration with shrinking of the BmN cell nucleus, and significant inhibition of nuclear DNA synthesis and cell proliferation. It also promoted autophagy via the lysosomal pathway, and accelerated apoptosis via the caspase pathway. |
| first_indexed | 2024-12-11T18:40:53Z |
| format | Article |
| id | doaj.art-81b1ea73934a44e283fef17b2987c91e |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-11T18:40:53Z |
| publishDate | 2019-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-81b1ea73934a44e283fef17b2987c91e2022-12-22T00:54:37ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2019-05-011010.3389/fphys.2019.00537450446Inhibition of Period Gene Expression Causes Repression of Cell Cycle Progression and Cell Growth in the Bombyx mori CellsJian-Feng Qiu0Jian-Feng Qiu1Xue Li2Xue Li3Wen-Zhao Cui4Wen-Zhao Cui5Xiao-Fei Liu6Xiao-Fei Liu7Hui Tao8Hui Tao9Kun Yang10Kun Yang11Tai-Ming Dai12Tai-Ming Dai13Yang-Hu Sima14Yang-Hu Sima15Shi-Qing Xu16Shi-Qing Xu17School of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, ChinaInstitute of Agricultural Biotechnology and Ecology (IABE), Soochow University, Suzhou, ChinaSchool of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, ChinaInstitute of Agricultural Biotechnology and Ecology (IABE), Soochow University, Suzhou, ChinaSchool of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, ChinaInstitute of Agricultural Biotechnology and Ecology (IABE), Soochow University, Suzhou, ChinaSchool of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, ChinaInstitute of Agricultural Biotechnology and Ecology (IABE), Soochow University, Suzhou, ChinaSchool of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, ChinaInstitute of Agricultural Biotechnology and Ecology (IABE), Soochow University, Suzhou, ChinaSchool of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, ChinaInstitute of Agricultural Biotechnology and Ecology (IABE), Soochow University, Suzhou, ChinaSchool of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, ChinaInstitute of Agricultural Biotechnology and Ecology (IABE), Soochow University, Suzhou, ChinaSchool of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, ChinaInstitute of Agricultural Biotechnology and Ecology (IABE), Soochow University, Suzhou, ChinaSchool of Biology and Basic Medical Sciences, Medical College, Soochow University, Suzhou, ChinaInstitute of Agricultural Biotechnology and Ecology (IABE), Soochow University, Suzhou, ChinaCircadian clock system disorders can lead to uncontrolled cell proliferation, but the molecular mechanism remains unknown. We used a Bombyx mori animal model of single Period gene (BmPer) expression to investigate this mechanism. A slow growing developmental cell model (Per-KD) was isolated from a B. mori ovarian cell line (BmN) by continuous knock down of BmPer expression. The effects of BmPer expression knockdown (Per-KD) on cell proliferation and apoptosis were opposite to those of m/hPer1 and m/hPer2 in mammals. The knockdown of BmPer expression led to cell cycle deceleration with shrinking of the BmN cell nucleus, and significant inhibition of nuclear DNA synthesis and cell proliferation. It also promoted autophagy via the lysosomal pathway, and accelerated apoptosis via the caspase pathway.https://www.frontiersin.org/article/10.3389/fphys.2019.00537/fullPeriod genecell cycleapoptosisautophagyovarian cell lineBombyx mori |
| spellingShingle | Jian-Feng Qiu Jian-Feng Qiu Xue Li Xue Li Wen-Zhao Cui Wen-Zhao Cui Xiao-Fei Liu Xiao-Fei Liu Hui Tao Hui Tao Kun Yang Kun Yang Tai-Ming Dai Tai-Ming Dai Yang-Hu Sima Yang-Hu Sima Shi-Qing Xu Shi-Qing Xu Inhibition of Period Gene Expression Causes Repression of Cell Cycle Progression and Cell Growth in the Bombyx mori Cells Frontiers in Physiology Period gene cell cycle apoptosis autophagy ovarian cell line Bombyx mori |
| title | Inhibition of Period Gene Expression Causes Repression of Cell Cycle Progression and Cell Growth in the Bombyx mori Cells |
| title_full | Inhibition of Period Gene Expression Causes Repression of Cell Cycle Progression and Cell Growth in the Bombyx mori Cells |
| title_fullStr | Inhibition of Period Gene Expression Causes Repression of Cell Cycle Progression and Cell Growth in the Bombyx mori Cells |
| title_full_unstemmed | Inhibition of Period Gene Expression Causes Repression of Cell Cycle Progression and Cell Growth in the Bombyx mori Cells |
| title_short | Inhibition of Period Gene Expression Causes Repression of Cell Cycle Progression and Cell Growth in the Bombyx mori Cells |
| title_sort | inhibition of period gene expression causes repression of cell cycle progression and cell growth in the bombyx mori cells |
| topic | Period gene cell cycle apoptosis autophagy ovarian cell line Bombyx mori |
| url | https://www.frontiersin.org/article/10.3389/fphys.2019.00537/full |
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