3D grating-based X-ray phase-contrast computed tomography for high-resolution quantitative assessment of cartilage: An experimental feasibility study with 3T MRI, 7T MRI and biomechanical correlation.
<h4>Objective</h4>Aim of this study was, to demonstrate the feasibility of high-resolution grating-based X-ray phase-contrast computed tomography (PCCT) for quantitative assessment of cartilage.<h4>Materials and methods</h4>In an experimental setup, 12 osteochondral samples w...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0212106 |
| _version_ | 1819112691001720832 |
|---|---|
| author | Julia Herzen Dimitrios C Karampinos Peter Foehr Lorenz Birnbacher Manuel Viermetz Rainer Burgkart Thomas Baum Fabian Lohoefer Moritz Wildgruber Franz Schilling Marian Willner Mathias Marschner Peter B Noël Ernst J Rummeny Franz Pfeiffer Pia M Jungmann |
| author_facet | Julia Herzen Dimitrios C Karampinos Peter Foehr Lorenz Birnbacher Manuel Viermetz Rainer Burgkart Thomas Baum Fabian Lohoefer Moritz Wildgruber Franz Schilling Marian Willner Mathias Marschner Peter B Noël Ernst J Rummeny Franz Pfeiffer Pia M Jungmann |
| author_sort | Julia Herzen |
| collection | DOAJ |
| description | <h4>Objective</h4>Aim of this study was, to demonstrate the feasibility of high-resolution grating-based X-ray phase-contrast computed tomography (PCCT) for quantitative assessment of cartilage.<h4>Materials and methods</h4>In an experimental setup, 12 osteochondral samples were harvested from n = 6 bovine knees (n = 2 each). From each knee, one cartilage sample was degraded using 2.5% Trypsin. In addition to PCCT and biomechanical cartilage stiffness measurements, 3T and 7T MRI was performed including MSME SE T2 and ME GE T2* mapping sequences for relaxationtime measurements. Paired t-tests and receiver operating characteristics (ROC) curves were used for statistical analyses.<h4>Results</h4>PCCT provided high-resolution images for improved morphological cartilage evaluation as compared to 3T and 7T MRI. Quantitative analyses revealed significant differences between the superficial and the deep cartilage layer for T2 mapping as well as for PCCT (P<0.05). No significant difference was detected for PCCT between healthy and degraded samples (P>0.05). MRI and stiffness measurements showed significant differences between healthy and degraded osteochondral samples. Accuracy in the prediction of cartilage degradation was excellent for MRI and biomechanical analyses.<h4>Conclusion</h4>In conclusion, high-resolution grating-based X-ray PCCT cartilage imaging is feasible. In addition to MRI and biomechanical analyses it provides complementary, water content independent, information for improved morphological and quantitative characterization of articular cartilage ultrastructure. |
| first_indexed | 2024-12-22T04:17:32Z |
| format | Article |
| id | doaj.art-81b27d522d04453d907bcc1361cd21ff |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T04:17:32Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-81b27d522d04453d907bcc1361cd21ff2022-12-21T18:39:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01142e021210610.1371/journal.pone.02121063D grating-based X-ray phase-contrast computed tomography for high-resolution quantitative assessment of cartilage: An experimental feasibility study with 3T MRI, 7T MRI and biomechanical correlation.Julia HerzenDimitrios C KarampinosPeter FoehrLorenz BirnbacherManuel ViermetzRainer BurgkartThomas BaumFabian LohoeferMoritz WildgruberFranz SchillingMarian WillnerMathias MarschnerPeter B NoëlErnst J RummenyFranz PfeifferPia M Jungmann<h4>Objective</h4>Aim of this study was, to demonstrate the feasibility of high-resolution grating-based X-ray phase-contrast computed tomography (PCCT) for quantitative assessment of cartilage.<h4>Materials and methods</h4>In an experimental setup, 12 osteochondral samples were harvested from n = 6 bovine knees (n = 2 each). From each knee, one cartilage sample was degraded using 2.5% Trypsin. In addition to PCCT and biomechanical cartilage stiffness measurements, 3T and 7T MRI was performed including MSME SE T2 and ME GE T2* mapping sequences for relaxationtime measurements. Paired t-tests and receiver operating characteristics (ROC) curves were used for statistical analyses.<h4>Results</h4>PCCT provided high-resolution images for improved morphological cartilage evaluation as compared to 3T and 7T MRI. Quantitative analyses revealed significant differences between the superficial and the deep cartilage layer for T2 mapping as well as for PCCT (P<0.05). No significant difference was detected for PCCT between healthy and degraded samples (P>0.05). MRI and stiffness measurements showed significant differences between healthy and degraded osteochondral samples. Accuracy in the prediction of cartilage degradation was excellent for MRI and biomechanical analyses.<h4>Conclusion</h4>In conclusion, high-resolution grating-based X-ray PCCT cartilage imaging is feasible. In addition to MRI and biomechanical analyses it provides complementary, water content independent, information for improved morphological and quantitative characterization of articular cartilage ultrastructure.https://doi.org/10.1371/journal.pone.0212106 |
| spellingShingle | Julia Herzen Dimitrios C Karampinos Peter Foehr Lorenz Birnbacher Manuel Viermetz Rainer Burgkart Thomas Baum Fabian Lohoefer Moritz Wildgruber Franz Schilling Marian Willner Mathias Marschner Peter B Noël Ernst J Rummeny Franz Pfeiffer Pia M Jungmann 3D grating-based X-ray phase-contrast computed tomography for high-resolution quantitative assessment of cartilage: An experimental feasibility study with 3T MRI, 7T MRI and biomechanical correlation. PLoS ONE |
| title | 3D grating-based X-ray phase-contrast computed tomography for high-resolution quantitative assessment of cartilage: An experimental feasibility study with 3T MRI, 7T MRI and biomechanical correlation. |
| title_full | 3D grating-based X-ray phase-contrast computed tomography for high-resolution quantitative assessment of cartilage: An experimental feasibility study with 3T MRI, 7T MRI and biomechanical correlation. |
| title_fullStr | 3D grating-based X-ray phase-contrast computed tomography for high-resolution quantitative assessment of cartilage: An experimental feasibility study with 3T MRI, 7T MRI and biomechanical correlation. |
| title_full_unstemmed | 3D grating-based X-ray phase-contrast computed tomography for high-resolution quantitative assessment of cartilage: An experimental feasibility study with 3T MRI, 7T MRI and biomechanical correlation. |
| title_short | 3D grating-based X-ray phase-contrast computed tomography for high-resolution quantitative assessment of cartilage: An experimental feasibility study with 3T MRI, 7T MRI and biomechanical correlation. |
| title_sort | 3d grating based x ray phase contrast computed tomography for high resolution quantitative assessment of cartilage an experimental feasibility study with 3t mri 7t mri and biomechanical correlation |
| url | https://doi.org/10.1371/journal.pone.0212106 |
| work_keys_str_mv | AT juliaherzen 3dgratingbasedxrayphasecontrastcomputedtomographyforhighresolutionquantitativeassessmentofcartilageanexperimentalfeasibilitystudywith3tmri7tmriandbiomechanicalcorrelation AT dimitriosckarampinos 3dgratingbasedxrayphasecontrastcomputedtomographyforhighresolutionquantitativeassessmentofcartilageanexperimentalfeasibilitystudywith3tmri7tmriandbiomechanicalcorrelation AT peterfoehr 3dgratingbasedxrayphasecontrastcomputedtomographyforhighresolutionquantitativeassessmentofcartilageanexperimentalfeasibilitystudywith3tmri7tmriandbiomechanicalcorrelation AT lorenzbirnbacher 3dgratingbasedxrayphasecontrastcomputedtomographyforhighresolutionquantitativeassessmentofcartilageanexperimentalfeasibilitystudywith3tmri7tmriandbiomechanicalcorrelation AT manuelviermetz 3dgratingbasedxrayphasecontrastcomputedtomographyforhighresolutionquantitativeassessmentofcartilageanexperimentalfeasibilitystudywith3tmri7tmriandbiomechanicalcorrelation AT rainerburgkart 3dgratingbasedxrayphasecontrastcomputedtomographyforhighresolutionquantitativeassessmentofcartilageanexperimentalfeasibilitystudywith3tmri7tmriandbiomechanicalcorrelation AT thomasbaum 3dgratingbasedxrayphasecontrastcomputedtomographyforhighresolutionquantitativeassessmentofcartilageanexperimentalfeasibilitystudywith3tmri7tmriandbiomechanicalcorrelation AT fabianlohoefer 3dgratingbasedxrayphasecontrastcomputedtomographyforhighresolutionquantitativeassessmentofcartilageanexperimentalfeasibilitystudywith3tmri7tmriandbiomechanicalcorrelation AT moritzwildgruber 3dgratingbasedxrayphasecontrastcomputedtomographyforhighresolutionquantitativeassessmentofcartilageanexperimentalfeasibilitystudywith3tmri7tmriandbiomechanicalcorrelation AT franzschilling 3dgratingbasedxrayphasecontrastcomputedtomographyforhighresolutionquantitativeassessmentofcartilageanexperimentalfeasibilitystudywith3tmri7tmriandbiomechanicalcorrelation AT marianwillner 3dgratingbasedxrayphasecontrastcomputedtomographyforhighresolutionquantitativeassessmentofcartilageanexperimentalfeasibilitystudywith3tmri7tmriandbiomechanicalcorrelation AT mathiasmarschner 3dgratingbasedxrayphasecontrastcomputedtomographyforhighresolutionquantitativeassessmentofcartilageanexperimentalfeasibilitystudywith3tmri7tmriandbiomechanicalcorrelation AT peterbnoel 3dgratingbasedxrayphasecontrastcomputedtomographyforhighresolutionquantitativeassessmentofcartilageanexperimentalfeasibilitystudywith3tmri7tmriandbiomechanicalcorrelation AT ernstjrummeny 3dgratingbasedxrayphasecontrastcomputedtomographyforhighresolutionquantitativeassessmentofcartilageanexperimentalfeasibilitystudywith3tmri7tmriandbiomechanicalcorrelation AT franzpfeiffer 3dgratingbasedxrayphasecontrastcomputedtomographyforhighresolutionquantitativeassessmentofcartilageanexperimentalfeasibilitystudywith3tmri7tmriandbiomechanicalcorrelation AT piamjungmann 3dgratingbasedxrayphasecontrastcomputedtomographyforhighresolutionquantitativeassessmentofcartilageanexperimentalfeasibilitystudywith3tmri7tmriandbiomechanicalcorrelation |