The NK1 receptor antagonist NKP608 inhibits proliferation of human colorectal cancer cells via Wnt signaling pathway
Abstract Background Neurokinin1 (NK1) receptor has played a vital role in the development of tumor. However, NKP608 as a NK1 receptor antagonist whether has the effect of the resistance of colorectal cancer is still unclear. Thereby, in this study, we investigated the role of NKP608 on human colorec...
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| Format: | Article |
| Language: | English |
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BMC
2018-05-01
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| Series: | Biological Research |
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| Online Access: | http://link.springer.com/article/10.1186/s40659-018-0163-x |
| _version_ | 1828891839834357760 |
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| author | Xiao-Ling Niu Jian-Feng Hou Jing-Xiang Li |
| author_facet | Xiao-Ling Niu Jian-Feng Hou Jing-Xiang Li |
| author_sort | Xiao-Ling Niu |
| collection | DOAJ |
| description | Abstract Background Neurokinin1 (NK1) receptor has played a vital role in the development of tumor. However, NKP608 as a NK1 receptor antagonist whether has the effect of the resistance of colorectal cancer is still unclear. Thereby, in this study, we investigated the role of NKP608 on human colorectal cancer and explored the underlying mechanism. Methods The cell proliferation of colorectal cancer cells was detected by cell counting kit-8 (CCK8) assay, cell migration and invasion were assessed by transwell assay, the apoptotic ratio of cells was assessed by Annexin V-fluorescein isothiocyanate/propidium iodide stained and flow cytometry. The involvement of molecular mechanisms was examined by western blot. Results In this study, we found that NKP608 inhibited the proliferation, migration/invasion of HCT116 cells. In addition, NKP608 reduced expressions of Wnt-3a, β-catenin, Cyclin D1, and (vascular endothelial growth factor) VEGF while induced expression of E-Cadherin. Furthermore, flow cytometry analyzed that NKP608 induced apoptosis of HCT116 cells, consistently, western blotting detecting of apoptosis-related proteins revealed that NKP608 downregulated Bcl-2 while upregulated Bax and Active-Caspase-3. Conclusions Taken together, our results demonstrated that NKP608 inhibited colorectal cancer cell proliferation, migration and invasion via suppressing the Wnt/β-catenin signaling pathway. Therefore, NKP608 might represent a promising therapeutic agent in the treatment of colorectal cancer. |
| first_indexed | 2024-12-13T13:25:11Z |
| format | Article |
| id | doaj.art-81b2cc1377e4425e804c50983221ccd2 |
| institution | Directory Open Access Journal |
| issn | 0717-6287 |
| language | English |
| last_indexed | 2024-12-13T13:25:11Z |
| publishDate | 2018-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-81b2cc1377e4425e804c50983221ccd22022-12-21T23:44:19ZengBMCBiological Research0717-62872018-05-015111810.1186/s40659-018-0163-xThe NK1 receptor antagonist NKP608 inhibits proliferation of human colorectal cancer cells via Wnt signaling pathwayXiao-Ling Niu0Jian-Feng Hou1Jing-Xiang Li2Department of Traditional Chinese Medicine, Shanghai Pudong New Area Zhoupu HospitalDepartment of Hepatobiliary Surgery, The First Hospital of Yulin CityAnorectal Department, Dongzhimen Hospital, Beijing University of Chinese MedicineAbstract Background Neurokinin1 (NK1) receptor has played a vital role in the development of tumor. However, NKP608 as a NK1 receptor antagonist whether has the effect of the resistance of colorectal cancer is still unclear. Thereby, in this study, we investigated the role of NKP608 on human colorectal cancer and explored the underlying mechanism. Methods The cell proliferation of colorectal cancer cells was detected by cell counting kit-8 (CCK8) assay, cell migration and invasion were assessed by transwell assay, the apoptotic ratio of cells was assessed by Annexin V-fluorescein isothiocyanate/propidium iodide stained and flow cytometry. The involvement of molecular mechanisms was examined by western blot. Results In this study, we found that NKP608 inhibited the proliferation, migration/invasion of HCT116 cells. In addition, NKP608 reduced expressions of Wnt-3a, β-catenin, Cyclin D1, and (vascular endothelial growth factor) VEGF while induced expression of E-Cadherin. Furthermore, flow cytometry analyzed that NKP608 induced apoptosis of HCT116 cells, consistently, western blotting detecting of apoptosis-related proteins revealed that NKP608 downregulated Bcl-2 while upregulated Bax and Active-Caspase-3. Conclusions Taken together, our results demonstrated that NKP608 inhibited colorectal cancer cell proliferation, migration and invasion via suppressing the Wnt/β-catenin signaling pathway. Therefore, NKP608 might represent a promising therapeutic agent in the treatment of colorectal cancer.http://link.springer.com/article/10.1186/s40659-018-0163-xNKP608Colorectal cancerWnt signaling pathwayProliferation |
| spellingShingle | Xiao-Ling Niu Jian-Feng Hou Jing-Xiang Li The NK1 receptor antagonist NKP608 inhibits proliferation of human colorectal cancer cells via Wnt signaling pathway Biological Research NKP608 Colorectal cancer Wnt signaling pathway Proliferation |
| title | The NK1 receptor antagonist NKP608 inhibits proliferation of human colorectal cancer cells via Wnt signaling pathway |
| title_full | The NK1 receptor antagonist NKP608 inhibits proliferation of human colorectal cancer cells via Wnt signaling pathway |
| title_fullStr | The NK1 receptor antagonist NKP608 inhibits proliferation of human colorectal cancer cells via Wnt signaling pathway |
| title_full_unstemmed | The NK1 receptor antagonist NKP608 inhibits proliferation of human colorectal cancer cells via Wnt signaling pathway |
| title_short | The NK1 receptor antagonist NKP608 inhibits proliferation of human colorectal cancer cells via Wnt signaling pathway |
| title_sort | nk1 receptor antagonist nkp608 inhibits proliferation of human colorectal cancer cells via wnt signaling pathway |
| topic | NKP608 Colorectal cancer Wnt signaling pathway Proliferation |
| url | http://link.springer.com/article/10.1186/s40659-018-0163-x |
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