Serotype Occurrence, Virulence Profiles, Antimicrobial Resistance and Molecular Characterization of <i>Salmonella</i> Isolated from Hospitalized Patients with Gastroenteritis in Great Tunisia between 2010 and 2020
Non-typhoid <i>Salmonella</i> is one of the major causes of food-borne infections worldwide. The aim of the current study is to determine the serotype occurrence, virulence factors and antimicrobial resistance patterns of <i>Salmonella</i> isolated from hospitalized patients....
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/3/526 |
| _version_ | 1797613859271344128 |
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| author | Walid Oueslati Mohamed Ridha Rjeibi Hayet Benyedem Mounir Jebali Fatma Souissi Rachid Selmi Mohamed Sélim El Asli Farouk Barguellil Abdelfettah Ettriqui |
| author_facet | Walid Oueslati Mohamed Ridha Rjeibi Hayet Benyedem Mounir Jebali Fatma Souissi Rachid Selmi Mohamed Sélim El Asli Farouk Barguellil Abdelfettah Ettriqui |
| author_sort | Walid Oueslati |
| collection | DOAJ |
| description | Non-typhoid <i>Salmonella</i> is one of the major causes of food-borne infections worldwide. The aim of the current study is to determine the serotype occurrence, virulence factors and antimicrobial resistance patterns of <i>Salmonella</i> isolated from hospitalized patients. The identification of <i>Salmonella</i> strains was performed according to REMIC, 2018. The susceptibility of <i>Salmonella</i> isolates was assessed against 20 antimicrobials using the disk diffusion method. Some virulence and antimicrobial resistance genes were identified using PCR. Among the 61 isolated <i>Salmonella</i> strains, seven serotypes were identified and all were positive for the virulence genes <i>invA</i>, <i>mgtC</i> and <i>sirA</i>. Critical resistance rates (>40%) were detected for tetracycline, nalidixic acid, amoxicillin and fluoroquinolones. However, resistances to ertapenem, ceftazidim, aztreonam and colistin were null. In addition, 33% of the isolated strains were multidrug-resistant (MDR). Moreover, 80% and 60% of <i>S</i>. Kentucky isolates were identified as fluoroquinolone-resistant and MDR strains, respectively. The <i>qnrB</i> gene was amplified in 63.2% of fluoroquinolone-resistant strains. The <i>dfrA1</i> gene was identified in 20% (4/20) of the trimethoprim-sulfamethoxazole resistant strains and the integrase Class 2 gene was amplified in only 8.2% (5/61) of the isolates. Our findings highlight the emergence of MDR <i>Salmonella</i> isolates. A rationalization of antimicrobial use is urgently recommended in both human and veterinary medicine. |
| first_indexed | 2024-03-11T07:01:36Z |
| format | Article |
| id | doaj.art-81b3ff196e0f4c0e875e1d80fff32810 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T07:01:36Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-81b3ff196e0f4c0e875e1d80fff328102023-11-17T09:14:16ZengMDPI AGAntibiotics2079-63822023-03-0112352610.3390/antibiotics12030526Serotype Occurrence, Virulence Profiles, Antimicrobial Resistance and Molecular Characterization of <i>Salmonella</i> Isolated from Hospitalized Patients with Gastroenteritis in Great Tunisia between 2010 and 2020Walid Oueslati0Mohamed Ridha Rjeibi1Hayet Benyedem2Mounir Jebali3Fatma Souissi4Rachid Selmi5Mohamed Sélim El Asli6Farouk Barguellil7Abdelfettah Ettriqui8Laboratory of Management of Animal Production’s Health and Quality, National School of Veterinary Medicine of Sidi Thabet, University Manouba, La Manouba 2010, TunisiaLaboratory of Parasitology, National School of Veterinary Medicine of Sidi Thabet, University Manouba, La Manouba 2010, TunisiaLaboratory of Parasitology, National School of Veterinary Medicine of Sidi Thabet, University Manouba, La Manouba 2010, TunisiaLaboratory of Management of Animal Production’s Health and Quality, National School of Veterinary Medicine of Sidi Thabet, University Manouba, La Manouba 2010, TunisiaLaboratory of Management of Animal Production’s Health and Quality, National School of Veterinary Medicine of Sidi Thabet, University Manouba, La Manouba 2010, TunisiaLaboratory of Microbiology, National School of Veterinary Medicine of Sidi Thabet, University Manouba, La Manouba 2010, TunisiaLaboratory of Microorganisms and Environment, Molecular Diagnostic Tools and Emerging and Re-Emerging Infections, Military Hospital of Tunis, University of Tunis El Manar, Tunis 1068, TunisiaLaboratory of Microorganisms and Environment, Molecular Diagnostic Tools and Emerging and Re-Emerging Infections, Military Hospital of Tunis, University of Tunis El Manar, Tunis 1068, TunisiaLaboratory of Management of Animal Production’s Health and Quality, National School of Veterinary Medicine of Sidi Thabet, University Manouba, La Manouba 2010, TunisiaNon-typhoid <i>Salmonella</i> is one of the major causes of food-borne infections worldwide. The aim of the current study is to determine the serotype occurrence, virulence factors and antimicrobial resistance patterns of <i>Salmonella</i> isolated from hospitalized patients. The identification of <i>Salmonella</i> strains was performed according to REMIC, 2018. The susceptibility of <i>Salmonella</i> isolates was assessed against 20 antimicrobials using the disk diffusion method. Some virulence and antimicrobial resistance genes were identified using PCR. Among the 61 isolated <i>Salmonella</i> strains, seven serotypes were identified and all were positive for the virulence genes <i>invA</i>, <i>mgtC</i> and <i>sirA</i>. Critical resistance rates (>40%) were detected for tetracycline, nalidixic acid, amoxicillin and fluoroquinolones. However, resistances to ertapenem, ceftazidim, aztreonam and colistin were null. In addition, 33% of the isolated strains were multidrug-resistant (MDR). Moreover, 80% and 60% of <i>S</i>. Kentucky isolates were identified as fluoroquinolone-resistant and MDR strains, respectively. The <i>qnrB</i> gene was amplified in 63.2% of fluoroquinolone-resistant strains. The <i>dfrA1</i> gene was identified in 20% (4/20) of the trimethoprim-sulfamethoxazole resistant strains and the integrase Class 2 gene was amplified in only 8.2% (5/61) of the isolates. Our findings highlight the emergence of MDR <i>Salmonella</i> isolates. A rationalization of antimicrobial use is urgently recommended in both human and veterinary medicine.https://www.mdpi.com/2079-6382/12/3/526<i>Salmonella</i>gastroenteritisserotypesvirulenceMDRTunisia |
| spellingShingle | Walid Oueslati Mohamed Ridha Rjeibi Hayet Benyedem Mounir Jebali Fatma Souissi Rachid Selmi Mohamed Sélim El Asli Farouk Barguellil Abdelfettah Ettriqui Serotype Occurrence, Virulence Profiles, Antimicrobial Resistance and Molecular Characterization of <i>Salmonella</i> Isolated from Hospitalized Patients with Gastroenteritis in Great Tunisia between 2010 and 2020 Antibiotics <i>Salmonella</i> gastroenteritis serotypes virulence MDR Tunisia |
| title | Serotype Occurrence, Virulence Profiles, Antimicrobial Resistance and Molecular Characterization of <i>Salmonella</i> Isolated from Hospitalized Patients with Gastroenteritis in Great Tunisia between 2010 and 2020 |
| title_full | Serotype Occurrence, Virulence Profiles, Antimicrobial Resistance and Molecular Characterization of <i>Salmonella</i> Isolated from Hospitalized Patients with Gastroenteritis in Great Tunisia between 2010 and 2020 |
| title_fullStr | Serotype Occurrence, Virulence Profiles, Antimicrobial Resistance and Molecular Characterization of <i>Salmonella</i> Isolated from Hospitalized Patients with Gastroenteritis in Great Tunisia between 2010 and 2020 |
| title_full_unstemmed | Serotype Occurrence, Virulence Profiles, Antimicrobial Resistance and Molecular Characterization of <i>Salmonella</i> Isolated from Hospitalized Patients with Gastroenteritis in Great Tunisia between 2010 and 2020 |
| title_short | Serotype Occurrence, Virulence Profiles, Antimicrobial Resistance and Molecular Characterization of <i>Salmonella</i> Isolated from Hospitalized Patients with Gastroenteritis in Great Tunisia between 2010 and 2020 |
| title_sort | serotype occurrence virulence profiles antimicrobial resistance and molecular characterization of i salmonella i isolated from hospitalized patients with gastroenteritis in great tunisia between 2010 and 2020 |
| topic | <i>Salmonella</i> gastroenteritis serotypes virulence MDR Tunisia |
| url | https://www.mdpi.com/2079-6382/12/3/526 |
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