Monoclonal antibody induced with inactived EV71-Hn2 virus protects mice against lethal EV71-Hn2 virus infection
<p>Abstract</p> <p>Background</p> <p>Enterovirus 71 (EV71) is a viral pathogen that belongs to the <it>Picornaviridae </it>family, EV71-infected children can develop severe neurological complications leading to rapid clinical deterioration and death.</p&g...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-05-01
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| Series: | Virology Journal |
| Online Access: | http://www.virologyj.com/content/7/1/106 |
| _version_ | 1811278664672739328 |
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| author | Lin Lei Si Bing-yin Wu Xiao-yan Luo Yan-jun Chang Guo-hui Zhu Qing-yu |
| author_facet | Lin Lei Si Bing-yin Wu Xiao-yan Luo Yan-jun Chang Guo-hui Zhu Qing-yu |
| author_sort | Lin Lei |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Enterovirus 71 (EV71) is a viral pathogen that belongs to the <it>Picornaviridae </it>family, EV71-infected children can develop severe neurological complications leading to rapid clinical deterioration and death.</p> <p>Results</p> <p>In this study, several monoclonal antibodies (MAbs) were produced by immunizing mice with the inactived EV71 Henan (Hn2) virus strain. The isolated MAbs were characterised by <it>in vitro </it>neutralizing analysis and peptide ELISA. ELISA assay showed that the neutralizing monoclonal antibody 4E8 specifically reacted with synthetic peptides which contain amino acid 240-250 and 250-260 of EV71 VP1. The <it>in vivo </it>protection assay showed that 4E8 can protect two-day-old BALB/c mice against the lethal challenge of EV71 virus.</p> <p>Conclusion</p> <p>The MAb 4E8 could be a promising candidate to be humanized and used for treatment of EV71 infection.</p> |
| first_indexed | 2024-04-13T00:39:49Z |
| format | Article |
| id | doaj.art-81b6b5f032cc48c8866693acf3122dc6 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-13T00:39:49Z |
| publishDate | 2010-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-81b6b5f032cc48c8866693acf3122dc62022-12-22T03:10:13ZengBMCVirology Journal1743-422X2010-05-017110610.1186/1743-422X-7-106Monoclonal antibody induced with inactived EV71-Hn2 virus protects mice against lethal EV71-Hn2 virus infectionLin LeiSi Bing-yinWu Xiao-yanLuo Yan-junChang Guo-huiZhu Qing-yu<p>Abstract</p> <p>Background</p> <p>Enterovirus 71 (EV71) is a viral pathogen that belongs to the <it>Picornaviridae </it>family, EV71-infected children can develop severe neurological complications leading to rapid clinical deterioration and death.</p> <p>Results</p> <p>In this study, several monoclonal antibodies (MAbs) were produced by immunizing mice with the inactived EV71 Henan (Hn2) virus strain. The isolated MAbs were characterised by <it>in vitro </it>neutralizing analysis and peptide ELISA. ELISA assay showed that the neutralizing monoclonal antibody 4E8 specifically reacted with synthetic peptides which contain amino acid 240-250 and 250-260 of EV71 VP1. The <it>in vivo </it>protection assay showed that 4E8 can protect two-day-old BALB/c mice against the lethal challenge of EV71 virus.</p> <p>Conclusion</p> <p>The MAb 4E8 could be a promising candidate to be humanized and used for treatment of EV71 infection.</p>http://www.virologyj.com/content/7/1/106 |
| spellingShingle | Lin Lei Si Bing-yin Wu Xiao-yan Luo Yan-jun Chang Guo-hui Zhu Qing-yu Monoclonal antibody induced with inactived EV71-Hn2 virus protects mice against lethal EV71-Hn2 virus infection Virology Journal |
| title | Monoclonal antibody induced with inactived EV71-Hn2 virus protects mice against lethal EV71-Hn2 virus infection |
| title_full | Monoclonal antibody induced with inactived EV71-Hn2 virus protects mice against lethal EV71-Hn2 virus infection |
| title_fullStr | Monoclonal antibody induced with inactived EV71-Hn2 virus protects mice against lethal EV71-Hn2 virus infection |
| title_full_unstemmed | Monoclonal antibody induced with inactived EV71-Hn2 virus protects mice against lethal EV71-Hn2 virus infection |
| title_short | Monoclonal antibody induced with inactived EV71-Hn2 virus protects mice against lethal EV71-Hn2 virus infection |
| title_sort | monoclonal antibody induced with inactived ev71 hn2 virus protects mice against lethal ev71 hn2 virus infection |
| url | http://www.virologyj.com/content/7/1/106 |
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