LINC00607 affects the proliferation and apoptosis of A549/DDP cells through inhibiting miR-372
Objective To explore the effect of LINC00607 on proliferation and apoptosis of lung cancer cisplatin-resistant cell line(A549/DDP) and its possible molecular mechanism. Methods The expressions of LINC00607 and miR-372 in lung cancer tissues, adjacent tissues, A549, A549/DDP cells were detected b...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-07-01
|
| Series: | Jichu yixue yu linchuang |
| Subjects: | |
| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200897.pdf |
| _version_ | 1797365793428602880 |
|---|---|
| author | YANG Wen-chuan, HAN Dong, YANG Fan |
| author_facet | YANG Wen-chuan, HAN Dong, YANG Fan |
| author_sort | YANG Wen-chuan, HAN Dong, YANG Fan |
| collection | DOAJ |
| description | Objective To explore the effect of LINC00607 on proliferation and apoptosis of lung cancer cisplatin-resistant cell line(A549/DDP) and its possible molecular mechanism. Methods The expressions of LINC00607 and miR-372 in lung cancer tissues, adjacent tissues, A549, A549/DDP cells were detected by real-time fluorescence quantitative PCR(RT-qOCR). A549/DDP cells were divided into pcDNA3.1 group, pcDNA3.1-LINC00607 group, (pcDNA3.1-LINC00607 + miR-NC)group, (pcDNA3.1-LINC00607 + miR-372)group. Methyl thiazolyl tetrazolium (MTT) assacy and colony formation experiments were used to test cell proliferation; flow cytometry was used to measure apoptosis; and Western blot was used to detect the expression levels of cleared-caspase-3 and Ki67; Dual luciferase reporter gene and RT-qPCR experiments confirmed the targeted regulation effect of LINC00607 on miR-372. Results Compared with adjacent tissues, the expression of LINC00607 in lung cancer tissues was significantly reduced, while the expression of miR-372 was significantly increased (P＜0.05). Compared with A549 cells, the expression of LINC00607 in A549/DDP cells was significantly reduced, while the expression of miR-372 was significantly increased (P＜0.05). Compared with pcDNA3.1 group, A549/DDP cell survival rate, clone formation numbers, Ki67 protein expression in pcDNA3.1-LINC00607 group were significantly decreased, and apoptosis rate and cleared-caspase-3 protein expression were significantly increased (P＜0.05). Compared with the (pcDNA3.1-LINC00607 + miR-NC)group, the A549/DDP cell survival rate, clone formation, Ki67 protein expression in the (pcDNA3.1-LINC00607 + miR-372)group were significantly increased, and the apoptosis rate, cleared-caspase-3 protein expression were significantly reduced (P＜0.05). LINC00607 targeted at and negatively regulated miR-372 expression. Conclusions LINC00607 inhibits proliferation and induces apoptosis of A549/DDP cells by down-regulating miR-372. |
| first_indexed | 2024-03-08T16:54:01Z |
| format | Article |
| id | doaj.art-81bb852c54074019b941629f11118766 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:54:01Z |
| publishDate | 2021-07-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-81bb852c54074019b941629f111187662024-01-05T03:05:36ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252021-07-0141710011006LINC00607 affects the proliferation and apoptosis of A549/DDP cells through inhibiting miR-372YANG Wen-chuan, HAN Dong, YANG Fan0Linyi Third People's Hospital, Linyi 276004, ChinaObjective To explore the effect of LINC00607 on proliferation and apoptosis of lung cancer cisplatin-resistant cell line(A549/DDP) and its possible molecular mechanism. Methods The expressions of LINC00607 and miR-372 in lung cancer tissues, adjacent tissues, A549, A549/DDP cells were detected by real-time fluorescence quantitative PCR(RT-qOCR). A549/DDP cells were divided into pcDNA3.1 group, pcDNA3.1-LINC00607 group, (pcDNA3.1-LINC00607 + miR-NC)group, (pcDNA3.1-LINC00607 + miR-372)group. Methyl thiazolyl tetrazolium (MTT) assacy and colony formation experiments were used to test cell proliferation; flow cytometry was used to measure apoptosis; and Western blot was used to detect the expression levels of cleared-caspase-3 and Ki67; Dual luciferase reporter gene and RT-qPCR experiments confirmed the targeted regulation effect of LINC00607 on miR-372. Results Compared with adjacent tissues, the expression of LINC00607 in lung cancer tissues was significantly reduced, while the expression of miR-372 was significantly increased (P＜0.05). Compared with A549 cells, the expression of LINC00607 in A549/DDP cells was significantly reduced, while the expression of miR-372 was significantly increased (P＜0.05). Compared with pcDNA3.1 group, A549/DDP cell survival rate, clone formation numbers, Ki67 protein expression in pcDNA3.1-LINC00607 group were significantly decreased, and apoptosis rate and cleared-caspase-3 protein expression were significantly increased (P＜0.05). Compared with the (pcDNA3.1-LINC00607 + miR-NC)group, the A549/DDP cell survival rate, clone formation, Ki67 protein expression in the (pcDNA3.1-LINC00607 + miR-372)group were significantly increased, and the apoptosis rate, cleared-caspase-3 protein expression were significantly reduced (P＜0.05). LINC00607 targeted at and negatively regulated miR-372 expression. Conclusions LINC00607 inhibits proliferation and induces apoptosis of A549/DDP cells by down-regulating miR-372.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200897.pdflinc00607|mir-372|a549/ddp cell line|drug resistance sensitivity |
| spellingShingle | YANG Wen-chuan, HAN Dong, YANG Fan LINC00607 affects the proliferation and apoptosis of A549/DDP cells through inhibiting miR-372 Jichu yixue yu linchuang linc00607|mir-372|a549/ddp cell line|drug resistance sensitivity |
| title | LINC00607 affects the proliferation and apoptosis of A549/DDP cells through inhibiting miR-372 |
| title_full | LINC00607 affects the proliferation and apoptosis of A549/DDP cells through inhibiting miR-372 |
| title_fullStr | LINC00607 affects the proliferation and apoptosis of A549/DDP cells through inhibiting miR-372 |
| title_full_unstemmed | LINC00607 affects the proliferation and apoptosis of A549/DDP cells through inhibiting miR-372 |
| title_short | LINC00607 affects the proliferation and apoptosis of A549/DDP cells through inhibiting miR-372 |
| title_sort | linc00607 affects the proliferation and apoptosis of a549 ddp cells through inhibiting mir 372 |
| topic | linc00607|mir-372|a549/ddp cell line|drug resistance sensitivity |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200897.pdf |
| work_keys_str_mv | AT yangwenchuanhandongyangfan linc00607affectstheproliferationandapoptosisofa549ddpcellsthroughinhibitingmir372 |