Effect of Danofloxacin Treatment on the Development of Fluoroquinolone Resistance in <i>Campylobacter jejuni</i> in Calves
<i>Campylobacter</i> is a leading cause of foodborne gastroenteritis. Recent studies have indicated a rise in fluoroquinolone-resistant (FQ-R) <i>Campylobacter</i> in cattle, where FQ is used to control bovine respiratory disease (BRD). To assess the effect of danofloxacin tr...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/4/531 |
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| author | Debora Brito Goulart Ashenafi Feyisa Beyi Zuowei Wu Mehmet Cemal Adiguzel Anastasia Schroeder Kritika Singh Changyun Xu Melda Meral Ocal Renee Dewell Grant A. Dewell Paul J. Plummer Qijing Zhang Orhan Sahin |
| author_facet | Debora Brito Goulart Ashenafi Feyisa Beyi Zuowei Wu Mehmet Cemal Adiguzel Anastasia Schroeder Kritika Singh Changyun Xu Melda Meral Ocal Renee Dewell Grant A. Dewell Paul J. Plummer Qijing Zhang Orhan Sahin |
| author_sort | Debora Brito Goulart |
| collection | DOAJ |
| description | <i>Campylobacter</i> is a leading cause of foodborne gastroenteritis. Recent studies have indicated a rise in fluoroquinolone-resistant (FQ-R) <i>Campylobacter</i> in cattle, where FQ is used to control bovine respiratory disease (BRD). To assess the effect of danofloxacin treatment on the development of FQ-resistance in <i>C. jejuni</i>, 30 commercial calves were divided into Group 1, Group 2, and Group 3 (<i>n</i> = 10), and were all inoculated orally with FQ-susceptible (FQ-S) <i>C. jejuni</i>; seven days later, Group 3 was challenged with transtracheal <i>Mannheimia haemolytica</i>, and one week later, Group 2 and Group 3 were injected subcutaneously with danofloxacin. Rectal feces were collected to determine relative percentages of FQ-R <i>Campylobacter</i> via culture. Before oral inoculation with <i>C. jejuni</i>, 87% of calves were naturally colonized by FQ-R <i>C. jejuni</i>. Two days after the inoculation, FQ-R <i>C. jejuni</i> decreased substantially in the majority of calves. Within 24 h of danofloxacin injection, almost all <i>C. jejuni</i> populations shifted to an FQ-R phenotype in both FQ-treated groups, which was only transitory, as FQ-S strains became predominant during later periods. Genotyping indicated that the spike seen in FQ-R <i>C. jejuni</i> populations following the injection was due mainly to enrichment of preexisting FQ-R <i>C. jejuni</i>, rather than development of de novo FQ resistance in susceptible strains. These results provide important insights into the dynamic changes of FQ-resistant <i>Campylobacter</i> in cattle in response to FQ treatment. |
| first_indexed | 2024-03-09T11:15:04Z |
| format | Article |
| id | doaj.art-81bcd1eacd0c40f08fb47cdc87eb83c2 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T11:15:04Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-81bcd1eacd0c40f08fb47cdc87eb83c22023-12-01T00:33:29ZengMDPI AGAntibiotics2079-63822022-04-0111453110.3390/antibiotics11040531Effect of Danofloxacin Treatment on the Development of Fluoroquinolone Resistance in <i>Campylobacter jejuni</i> in CalvesDebora Brito Goulart0Ashenafi Feyisa Beyi1Zuowei Wu2Mehmet Cemal Adiguzel3Anastasia Schroeder4Kritika Singh5Changyun Xu6Melda Meral Ocal7Renee Dewell8Grant A. Dewell9Paul J. Plummer10Qijing Zhang11Orhan Sahin12Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USACenter for Food Security and Public Health, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA<i>Campylobacter</i> is a leading cause of foodborne gastroenteritis. Recent studies have indicated a rise in fluoroquinolone-resistant (FQ-R) <i>Campylobacter</i> in cattle, where FQ is used to control bovine respiratory disease (BRD). To assess the effect of danofloxacin treatment on the development of FQ-resistance in <i>C. jejuni</i>, 30 commercial calves were divided into Group 1, Group 2, and Group 3 (<i>n</i> = 10), and were all inoculated orally with FQ-susceptible (FQ-S) <i>C. jejuni</i>; seven days later, Group 3 was challenged with transtracheal <i>Mannheimia haemolytica</i>, and one week later, Group 2 and Group 3 were injected subcutaneously with danofloxacin. Rectal feces were collected to determine relative percentages of FQ-R <i>Campylobacter</i> via culture. Before oral inoculation with <i>C. jejuni</i>, 87% of calves were naturally colonized by FQ-R <i>C. jejuni</i>. Two days after the inoculation, FQ-R <i>C. jejuni</i> decreased substantially in the majority of calves. Within 24 h of danofloxacin injection, almost all <i>C. jejuni</i> populations shifted to an FQ-R phenotype in both FQ-treated groups, which was only transitory, as FQ-S strains became predominant during later periods. Genotyping indicated that the spike seen in FQ-R <i>C. jejuni</i> populations following the injection was due mainly to enrichment of preexisting FQ-R <i>C. jejuni</i>, rather than development of de novo FQ resistance in susceptible strains. These results provide important insights into the dynamic changes of FQ-resistant <i>Campylobacter</i> in cattle in response to FQ treatment.https://www.mdpi.com/2079-6382/11/4/531<i>Campylobacter</i>cattlecolonizationrectal fecesbovine respiratory disease (BRD)fluoroquinolone/danofloxacin treatment |
| spellingShingle | Debora Brito Goulart Ashenafi Feyisa Beyi Zuowei Wu Mehmet Cemal Adiguzel Anastasia Schroeder Kritika Singh Changyun Xu Melda Meral Ocal Renee Dewell Grant A. Dewell Paul J. Plummer Qijing Zhang Orhan Sahin Effect of Danofloxacin Treatment on the Development of Fluoroquinolone Resistance in <i>Campylobacter jejuni</i> in Calves Antibiotics <i>Campylobacter</i> cattle colonization rectal feces bovine respiratory disease (BRD) fluoroquinolone/danofloxacin treatment |
| title | Effect of Danofloxacin Treatment on the Development of Fluoroquinolone Resistance in <i>Campylobacter jejuni</i> in Calves |
| title_full | Effect of Danofloxacin Treatment on the Development of Fluoroquinolone Resistance in <i>Campylobacter jejuni</i> in Calves |
| title_fullStr | Effect of Danofloxacin Treatment on the Development of Fluoroquinolone Resistance in <i>Campylobacter jejuni</i> in Calves |
| title_full_unstemmed | Effect of Danofloxacin Treatment on the Development of Fluoroquinolone Resistance in <i>Campylobacter jejuni</i> in Calves |
| title_short | Effect of Danofloxacin Treatment on the Development of Fluoroquinolone Resistance in <i>Campylobacter jejuni</i> in Calves |
| title_sort | effect of danofloxacin treatment on the development of fluoroquinolone resistance in i campylobacter jejuni i in calves |
| topic | <i>Campylobacter</i> cattle colonization rectal feces bovine respiratory disease (BRD) fluoroquinolone/danofloxacin treatment |
| url | https://www.mdpi.com/2079-6382/11/4/531 |
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