Efficacy of Ciprofloxacin, Metronidazole and Minocycline in Ordered Mesoporous Silica against <i>Enterococcus faecalis</i> for Dental Pulp Revascularization: An In-Vitro Study
Pulp revascularization of teeth with necrotic pulp has become an alternative treatment in cases with immature apex. Microbial control is essential to achieve a successful outcome and continued root development. <i>Enterococcus faecalis</i> (<i>E. faecalis</i>) is the most fre...
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MDPI AG
2022-03-01
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| Online Access: | https://www.mdpi.com/1996-1944/15/6/2266 |
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| author | Cintia Micaela Chamorro-Petronacci Beatriz Santos Torres Rocío Guerrero-Nieves Mario Pérez-Sayáns Marcia Carvalho-de Abreu Fantini Luis Carlos Cides-da-Silva Beatriz Magariños Berta Rivas-Mundiña |
| author_facet | Cintia Micaela Chamorro-Petronacci Beatriz Santos Torres Rocío Guerrero-Nieves Mario Pérez-Sayáns Marcia Carvalho-de Abreu Fantini Luis Carlos Cides-da-Silva Beatriz Magariños Berta Rivas-Mundiña |
| author_sort | Cintia Micaela Chamorro-Petronacci |
| collection | DOAJ |
| description | Pulp revascularization of teeth with necrotic pulp has become an alternative treatment in cases with immature apex. Microbial control is essential to achieve a successful outcome and continued root development. <i>Enterococcus faecalis</i> (<i>E. faecalis</i>) is the most frequently isolated bacterial species in root canals of endodontically failed teeth. Our main goal was to compare the in-vitro antimicrobial efficacy of different antibiotic formulations delivered by ordered mesoporous silica (OMS) against <i>E. faecalis</i>. To determine antibiotic susceptibility, we tested OMS and triple antibiotic paste (TAP; ciprofloxacin:metronidazole:minocycline) with different reagents in different concentrations, using the Kirby–Bauer disk diffusion method. OMS and metronidazole showed no antibacterial activity against <i>E. faecalis</i>. Mixtures of OMS and antibiotics in proportions of 2:2:14 and 4:1:7 (mg/L of ciprofloxacin:metronidazole:minocycline, respectively) showed the lowest antibacterial activity. The antibacterial activity of the combined solutions of ciprofloxacin and metronidazole was significantly higher (<i>p</i> < 0.005). Combinations in different concentrations of minocycline, ciprofloxacin, and metronidazole in OMS have shown activity against <i>E. faecalis</i>, although the combined use of ciprofloxacin and metronidazole has shown the most effective results. This study demonstrates the efficacy of intracanal antibiotic combination paste activity against <i>E. faecalis,</i> avoiding the use of minocycline, whose undesirable effect of teeth staining is a common problem for patients and professionals in dental clinic. |
| first_indexed | 2024-03-09T13:27:21Z |
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| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T13:27:21Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-81bd56e671d34d87890b5c89317f476c2023-11-30T21:22:01ZengMDPI AGMaterials1996-19442022-03-01156226610.3390/ma15062266Efficacy of Ciprofloxacin, Metronidazole and Minocycline in Ordered Mesoporous Silica against <i>Enterococcus faecalis</i> for Dental Pulp Revascularization: An In-Vitro StudyCintia Micaela Chamorro-Petronacci0Beatriz Santos Torres1Rocío Guerrero-Nieves2Mario Pérez-Sayáns3Marcia Carvalho-de Abreu Fantini4Luis Carlos Cides-da-Silva5Beatriz Magariños6Berta Rivas-Mundiña7ORALRES Group, Department of Oral Medicine, Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Travesía da Choupana S/N, 15706 Santiago de Compostela, SpainDepartment of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Campus Vida, 15705 Santiago de Compostela, SpainMedOralRes Group, Department of Surgery and Medical-Surgical Specialities, Universidade de Santiago de Compostela (USC), Entrerrios S/N, 15705 Santiago de Compostela, SpainORALRES Group, Department of Oral Medicine, Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Travesía da Choupana S/N, 15706 Santiago de Compostela, SpainInstitute of Physics, Universidade de São Paulo, Rua do Matão, Sao Paulo 05508-090, SP, BrazilInstitute of Physics, Universidade de São Paulo, Rua do Matão, Sao Paulo 05508-090, SP, BrazilDepartment of Microbiology and Parasitology, CIBUS-Faculty of Biology, University of Santiago de Compostela, Campus Vida, 15705 Santiago de Compostela, SpainMedOralRes Group, Department of Surgery and Medical-Surgical Specialities, Universidade de Santiago de Compostela (USC), Entrerrios S/N, 15705 Santiago de Compostela, SpainPulp revascularization of teeth with necrotic pulp has become an alternative treatment in cases with immature apex. Microbial control is essential to achieve a successful outcome and continued root development. <i>Enterococcus faecalis</i> (<i>E. faecalis</i>) is the most frequently isolated bacterial species in root canals of endodontically failed teeth. Our main goal was to compare the in-vitro antimicrobial efficacy of different antibiotic formulations delivered by ordered mesoporous silica (OMS) against <i>E. faecalis</i>. To determine antibiotic susceptibility, we tested OMS and triple antibiotic paste (TAP; ciprofloxacin:metronidazole:minocycline) with different reagents in different concentrations, using the Kirby–Bauer disk diffusion method. OMS and metronidazole showed no antibacterial activity against <i>E. faecalis</i>. Mixtures of OMS and antibiotics in proportions of 2:2:14 and 4:1:7 (mg/L of ciprofloxacin:metronidazole:minocycline, respectively) showed the lowest antibacterial activity. The antibacterial activity of the combined solutions of ciprofloxacin and metronidazole was significantly higher (<i>p</i> < 0.005). Combinations in different concentrations of minocycline, ciprofloxacin, and metronidazole in OMS have shown activity against <i>E. faecalis</i>, although the combined use of ciprofloxacin and metronidazole has shown the most effective results. This study demonstrates the efficacy of intracanal antibiotic combination paste activity against <i>E. faecalis,</i> avoiding the use of minocycline, whose undesirable effect of teeth staining is a common problem for patients and professionals in dental clinic.https://www.mdpi.com/1996-1944/15/6/2266antibiotics<i>Enterococcus faecalis</i>microbial sensitivity testspulp revascularization |
| spellingShingle | Cintia Micaela Chamorro-Petronacci Beatriz Santos Torres Rocío Guerrero-Nieves Mario Pérez-Sayáns Marcia Carvalho-de Abreu Fantini Luis Carlos Cides-da-Silva Beatriz Magariños Berta Rivas-Mundiña Efficacy of Ciprofloxacin, Metronidazole and Minocycline in Ordered Mesoporous Silica against <i>Enterococcus faecalis</i> for Dental Pulp Revascularization: An In-Vitro Study Materials antibiotics <i>Enterococcus faecalis</i> microbial sensitivity tests pulp revascularization |
| title | Efficacy of Ciprofloxacin, Metronidazole and Minocycline in Ordered Mesoporous Silica against <i>Enterococcus faecalis</i> for Dental Pulp Revascularization: An In-Vitro Study |
| title_full | Efficacy of Ciprofloxacin, Metronidazole and Minocycline in Ordered Mesoporous Silica against <i>Enterococcus faecalis</i> for Dental Pulp Revascularization: An In-Vitro Study |
| title_fullStr | Efficacy of Ciprofloxacin, Metronidazole and Minocycline in Ordered Mesoporous Silica against <i>Enterococcus faecalis</i> for Dental Pulp Revascularization: An In-Vitro Study |
| title_full_unstemmed | Efficacy of Ciprofloxacin, Metronidazole and Minocycline in Ordered Mesoporous Silica against <i>Enterococcus faecalis</i> for Dental Pulp Revascularization: An In-Vitro Study |
| title_short | Efficacy of Ciprofloxacin, Metronidazole and Minocycline in Ordered Mesoporous Silica against <i>Enterococcus faecalis</i> for Dental Pulp Revascularization: An In-Vitro Study |
| title_sort | efficacy of ciprofloxacin metronidazole and minocycline in ordered mesoporous silica against i enterococcus faecalis i for dental pulp revascularization an in vitro study |
| topic | antibiotics <i>Enterococcus faecalis</i> microbial sensitivity tests pulp revascularization |
| url | https://www.mdpi.com/1996-1944/15/6/2266 |
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