Zinc and Iron Concentration as Affected by Nitrogen Fertilization and Their Localization in Wheat Grain
Nearly half of the world cereal production comes from soils low or marginal in plant available zinc, leading to unsustainable and poor quality grain production. Therefore, the effects of nitrogen (N) rate and application time on zinc (Zn) and iron (Fe) concentration in wheat grain were investigated....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2018.00307/full |
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| author | Bal R. Singh Yadu N. Timsina Ole C. Lind Simone Cagno Simone Cagno Koen Janssens |
| author_facet | Bal R. Singh Yadu N. Timsina Ole C. Lind Simone Cagno Simone Cagno Koen Janssens |
| author_sort | Bal R. Singh |
| collection | DOAJ |
| description | Nearly half of the world cereal production comes from soils low or marginal in plant available zinc, leading to unsustainable and poor quality grain production. Therefore, the effects of nitrogen (N) rate and application time on zinc (Zn) and iron (Fe) concentration in wheat grain were investigated. Wheat (Triticum aestivum var. Krabat) was grown in a growth chamber with 8 and 16 h of day and night periods, respectively. The N rates were 29, 43, and 57 mg N kg-1 soil, equivalent to 80, 120, and 160 kg N ha-1. Zinc and Fe were applied at 10 mg kg-1 growth media. In one of the N treatments, additional Zn and Fe through foliar spray (6 mg of Zn or Fe in 10 ml water/pot) was applied. Micro-analytical localization of Zn and Fe within grain was performed using scanning macro-X-ray fluorescence (MA-XRF) and laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). The following data were obtained: grain and straw yield pot-1, 1000 grains weight, number of grains pot-1, whole grain protein content, concentration of Zn and Fe in the grains. Grain yield increased from 80 to 120 kg N ha-1 rates only and decreased at 160 kg N ha-1 g. Relatively higher protein content and Zn and Fe concentration in the grain were recorded with the split N application of 160 kg N ha-1. Soil and foliar supply of Zn and Fe (Zn + Fes+f), with a single application of 120 kg N ha-1N at sowing, increased the concentration of Zn by 46% and of Fe by 35%, as compared to their growth media application only. Line scans of freshly cut areas of sliced grains showed co-localization of Zn and Fe within germ, crease and aleurone. We thus conclude that split application of N at 160 kg ha-1 at sowing and stem elongation, in combination with soil and foliar application of Zn and Fe, can be a good agricultural practice to enhance protein content and the Zn and Fe concentration in grain. |
| first_indexed | 2024-12-12T16:49:07Z |
| format | Article |
| id | doaj.art-81bd67261bbc4376b39582b9c90788af |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-12T16:49:07Z |
| publishDate | 2018-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-81bd67261bbc4376b39582b9c90788af2022-12-22T00:18:25ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-03-01910.3389/fpls.2018.00307327079Zinc and Iron Concentration as Affected by Nitrogen Fertilization and Their Localization in Wheat GrainBal R. Singh0Yadu N. Timsina1Ole C. Lind2Simone Cagno3Simone Cagno4Koen Janssens5Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, Ås, NorwayFaculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, Ås, NorwayCentre of Environmental Radioactivity, Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, Ås, NorwayCentre of Environmental Radioactivity, Faculty of Environmental Sciences and Natural Resource Management, Norwegian University of Life Sciences, Ås, NorwayDepartment of Chemistry, University of Antwerp, Antwerp, BelgiumDepartment of Chemistry, University of Antwerp, Antwerp, BelgiumNearly half of the world cereal production comes from soils low or marginal in plant available zinc, leading to unsustainable and poor quality grain production. Therefore, the effects of nitrogen (N) rate and application time on zinc (Zn) and iron (Fe) concentration in wheat grain were investigated. Wheat (Triticum aestivum var. Krabat) was grown in a growth chamber with 8 and 16 h of day and night periods, respectively. The N rates were 29, 43, and 57 mg N kg-1 soil, equivalent to 80, 120, and 160 kg N ha-1. Zinc and Fe were applied at 10 mg kg-1 growth media. In one of the N treatments, additional Zn and Fe through foliar spray (6 mg of Zn or Fe in 10 ml water/pot) was applied. Micro-analytical localization of Zn and Fe within grain was performed using scanning macro-X-ray fluorescence (MA-XRF) and laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). The following data were obtained: grain and straw yield pot-1, 1000 grains weight, number of grains pot-1, whole grain protein content, concentration of Zn and Fe in the grains. Grain yield increased from 80 to 120 kg N ha-1 rates only and decreased at 160 kg N ha-1 g. Relatively higher protein content and Zn and Fe concentration in the grain were recorded with the split N application of 160 kg N ha-1. Soil and foliar supply of Zn and Fe (Zn + Fes+f), with a single application of 120 kg N ha-1N at sowing, increased the concentration of Zn by 46% and of Fe by 35%, as compared to their growth media application only. Line scans of freshly cut areas of sliced grains showed co-localization of Zn and Fe within germ, crease and aleurone. We thus conclude that split application of N at 160 kg ha-1 at sowing and stem elongation, in combination with soil and foliar application of Zn and Fe, can be a good agricultural practice to enhance protein content and the Zn and Fe concentration in grain.http://journal.frontiersin.org/article/10.3389/fpls.2018.00307/fullnitrogen applicationzinc and iron uptakezinc and iron distribution in grainwheatLA-ICP-MSMA-XRF |
| spellingShingle | Bal R. Singh Yadu N. Timsina Ole C. Lind Simone Cagno Simone Cagno Koen Janssens Zinc and Iron Concentration as Affected by Nitrogen Fertilization and Their Localization in Wheat Grain Frontiers in Plant Science nitrogen application zinc and iron uptake zinc and iron distribution in grain wheat LA-ICP-MS MA-XRF |
| title | Zinc and Iron Concentration as Affected by Nitrogen Fertilization and Their Localization in Wheat Grain |
| title_full | Zinc and Iron Concentration as Affected by Nitrogen Fertilization and Their Localization in Wheat Grain |
| title_fullStr | Zinc and Iron Concentration as Affected by Nitrogen Fertilization and Their Localization in Wheat Grain |
| title_full_unstemmed | Zinc and Iron Concentration as Affected by Nitrogen Fertilization and Their Localization in Wheat Grain |
| title_short | Zinc and Iron Concentration as Affected by Nitrogen Fertilization and Their Localization in Wheat Grain |
| title_sort | zinc and iron concentration as affected by nitrogen fertilization and their localization in wheat grain |
| topic | nitrogen application zinc and iron uptake zinc and iron distribution in grain wheat LA-ICP-MS MA-XRF |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2018.00307/full |
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