Two Ecdysteroids Isolated from Micropropagated <i>Lychnis flos-cuculi</i> and the Biological Activity of Plant Material
Genetically uniform plant material, derived from <i>Lychnis flos-cuculi</i> propagated in vitro, was used for the isolation of 20-hydroxyecdysone and polypodine B and subjected to an evaluation of the antifungal and antiamoebic activity. The activity of 80% aqueous methanolic extracts, t...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/4/904 |
| _version_ | 1797412379109097472 |
|---|---|
| author | Michał P. Maliński Jaromir Budzianowski Małgorzata Kikowska Monika Derda Marcelina M. Jaworska Dariusz T. Mlynarczyk Marta Szukalska Ewa Florek Barbara Thiem |
| author_facet | Michał P. Maliński Jaromir Budzianowski Małgorzata Kikowska Monika Derda Marcelina M. Jaworska Dariusz T. Mlynarczyk Marta Szukalska Ewa Florek Barbara Thiem |
| author_sort | Michał P. Maliński |
| collection | DOAJ |
| description | Genetically uniform plant material, derived from <i>Lychnis flos-cuculi</i> propagated in vitro, was used for the isolation of 20-hydroxyecdysone and polypodine B and subjected to an evaluation of the antifungal and antiamoebic activity. The activity of 80% aqueous methanolic extracts, their fractions, and isolated ecdysteroids were studied against pathogenic <i>Acanthamoeba castellani</i>. Additionally, a Microtox<sup>®</sup> acute toxicity assay was performed. It was found that an 80% methanolic fraction of root extract exerts the most potent amoebicidal activity at IC<sub>50</sub> of 0.06 mg/mL at the 3rd day of treatment. Both ecdysteroids show comparable activity at IC<sub>50</sub> of 0.07 mg/mL. The acute toxicity of 80% fractions at similar concentrations is significantly higher than that of 40% fractions. Crude extracts exhibited moderate antifungal activity, with a minimum inhibitory concentration (MIC) within the range of 1.25–2.5 mg/mL. To the best of our knowledge, the present report is the first to show the biological activity of <i>L. flos-cuculi</i> in terms of the antifungal and antiamoebic activities and acute toxicity. It is also the first isolation of the main ecdysteroids from <i>L. flos-cuculi</i> micropropagated, ecdysteroid-rich plant material. |
| first_indexed | 2024-03-09T05:02:08Z |
| format | Article |
| id | doaj.art-81be253ac51c408a9ee9991f09d7e4de |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T05:02:08Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-81be253ac51c408a9ee9991f09d7e4de2023-12-03T12:58:52ZengMDPI AGMolecules1420-30492021-02-0126490410.3390/molecules26040904Two Ecdysteroids Isolated from Micropropagated <i>Lychnis flos-cuculi</i> and the Biological Activity of Plant MaterialMichał P. Maliński0Jaromir Budzianowski1Małgorzata Kikowska2Monika Derda3Marcelina M. Jaworska4Dariusz T. Mlynarczyk5Marta Szukalska6Ewa Florek7Barbara Thiem8Chair and Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, 14 Sw. Marii Magdaleny St., 61-861 Poznań, PolandChair and Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, 14 Sw. Marii Magdaleny St., 61-861 Poznań, PolandChair and Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, 14 Sw. Marii Magdaleny St., 61-861 Poznań, PolandChair and Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, 10 Fredry St., 61-701 Poznań, PolandChair and Department of Genetics and Pharmaceutical Microbiology, Poznan University of Medical Sciences, 4 Święcickiego St., 60-781 Poznań, PolandChair and Department of Chemical Technology of Drugs, Poznan University of Medical Sciences, 6 Grunwaldzka St., 60-780 Poznań, PolandLaboratory of Environmental Research, Chair and Department of Toxicology, Poznan University of Medical Sciences, 30 Dojazd St., 60-631 Poznań, PolandLaboratory of Environmental Research, Chair and Department of Toxicology, Poznan University of Medical Sciences, 30 Dojazd St., 60-631 Poznań, PolandChair and Department of Pharmaceutical Botany and Plant Biotechnology, Poznan University of Medical Sciences, 14 Sw. Marii Magdaleny St., 61-861 Poznań, PolandGenetically uniform plant material, derived from <i>Lychnis flos-cuculi</i> propagated in vitro, was used for the isolation of 20-hydroxyecdysone and polypodine B and subjected to an evaluation of the antifungal and antiamoebic activity. The activity of 80% aqueous methanolic extracts, their fractions, and isolated ecdysteroids were studied against pathogenic <i>Acanthamoeba castellani</i>. Additionally, a Microtox<sup>®</sup> acute toxicity assay was performed. It was found that an 80% methanolic fraction of root extract exerts the most potent amoebicidal activity at IC<sub>50</sub> of 0.06 mg/mL at the 3rd day of treatment. Both ecdysteroids show comparable activity at IC<sub>50</sub> of 0.07 mg/mL. The acute toxicity of 80% fractions at similar concentrations is significantly higher than that of 40% fractions. Crude extracts exhibited moderate antifungal activity, with a minimum inhibitory concentration (MIC) within the range of 1.25–2.5 mg/mL. To the best of our knowledge, the present report is the first to show the biological activity of <i>L. flos-cuculi</i> in terms of the antifungal and antiamoebic activities and acute toxicity. It is also the first isolation of the main ecdysteroids from <i>L. flos-cuculi</i> micropropagated, ecdysteroid-rich plant material.https://www.mdpi.com/1420-3049/26/4/90420-hydroxyecdysonepolypodine BRagged Robinplant tissue culture<i>Acanthamoeba</i>antifungal activity |
| spellingShingle | Michał P. Maliński Jaromir Budzianowski Małgorzata Kikowska Monika Derda Marcelina M. Jaworska Dariusz T. Mlynarczyk Marta Szukalska Ewa Florek Barbara Thiem Two Ecdysteroids Isolated from Micropropagated <i>Lychnis flos-cuculi</i> and the Biological Activity of Plant Material Molecules 20-hydroxyecdysone polypodine B Ragged Robin plant tissue culture <i>Acanthamoeba</i> antifungal activity |
| title | Two Ecdysteroids Isolated from Micropropagated <i>Lychnis flos-cuculi</i> and the Biological Activity of Plant Material |
| title_full | Two Ecdysteroids Isolated from Micropropagated <i>Lychnis flos-cuculi</i> and the Biological Activity of Plant Material |
| title_fullStr | Two Ecdysteroids Isolated from Micropropagated <i>Lychnis flos-cuculi</i> and the Biological Activity of Plant Material |
| title_full_unstemmed | Two Ecdysteroids Isolated from Micropropagated <i>Lychnis flos-cuculi</i> and the Biological Activity of Plant Material |
| title_short | Two Ecdysteroids Isolated from Micropropagated <i>Lychnis flos-cuculi</i> and the Biological Activity of Plant Material |
| title_sort | two ecdysteroids isolated from micropropagated i lychnis flos cuculi i and the biological activity of plant material |
| topic | 20-hydroxyecdysone polypodine B Ragged Robin plant tissue culture <i>Acanthamoeba</i> antifungal activity |
| url | https://www.mdpi.com/1420-3049/26/4/904 |
| work_keys_str_mv | AT michałpmalinski twoecdysteroidsisolatedfrommicropropagatedilychnisfloscuculiiandthebiologicalactivityofplantmaterial AT jaromirbudzianowski twoecdysteroidsisolatedfrommicropropagatedilychnisfloscuculiiandthebiologicalactivityofplantmaterial AT małgorzatakikowska twoecdysteroidsisolatedfrommicropropagatedilychnisfloscuculiiandthebiologicalactivityofplantmaterial AT monikaderda twoecdysteroidsisolatedfrommicropropagatedilychnisfloscuculiiandthebiologicalactivityofplantmaterial AT marcelinamjaworska twoecdysteroidsisolatedfrommicropropagatedilychnisfloscuculiiandthebiologicalactivityofplantmaterial AT dariusztmlynarczyk twoecdysteroidsisolatedfrommicropropagatedilychnisfloscuculiiandthebiologicalactivityofplantmaterial AT martaszukalska twoecdysteroidsisolatedfrommicropropagatedilychnisfloscuculiiandthebiologicalactivityofplantmaterial AT ewaflorek twoecdysteroidsisolatedfrommicropropagatedilychnisfloscuculiiandthebiologicalactivityofplantmaterial AT barbarathiem twoecdysteroidsisolatedfrommicropropagatedilychnisfloscuculiiandthebiologicalactivityofplantmaterial |