Detection of the antibacterial efficacy of Paenibacillus polymyxa against Xanthomonas oryzae pv. oryzae in rice seedlings, and the antimicrobial related genes
Paenibacillus polymyxa bacterium has been known as a biocontrol agent (BCA) for various plant diseases, and it promotes the growth of several treated plants. On the other hand, Xanthomonas oryzae pv. oryzae (Xoo) is an important bacterial pathogen that causes bacterial leaf blight (BLB) to rice pl...
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| Format: | Article |
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The National Information and Documentation Centre (NIDOC) , Egypt
2020-08-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://nrmj.journals.ekb.eg/article_107545_202ea527eb3f1c09f5522bd0250ddfff.pdf |
| _version_ | 1797204264256274432 |
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| author | Yasmine Abdallah Ranya M. El-Ashmony Abdelrazek S. Abdelrhim |
| author_facet | Yasmine Abdallah Ranya M. El-Ashmony Abdelrazek S. Abdelrhim |
| author_sort | Yasmine Abdallah |
| collection | DOAJ |
| description | Paenibacillus polymyxa bacterium has been known as a biocontrol agent (BCA) for various plant diseases,
and it promotes the growth of several treated plants. On the other hand, Xanthomonas oryzae pv. oryzae (Xoo) is
an important bacterial pathogen that causes bacterial leaf blight (BLB) to rice plants, and cause high yield loss
during the growing season. In this study, P. polymyxa SX3 inhibited the in vitro growth of Xoo by 80% and
affected the biofilm formation. Also, the cell membrane of Xoo lost its permeability after treating it with culture
filtrate of P. polymyxa at its MIC (40%). Transmission electron microscopic (TEM) observations showed an
influence of the culture filtrate of P. polymyxa on the cell morphology of Xoo. Applying P. polymyxa decreased
the severity of bacterial leaf blight on rice seedlings to 4.9%, compared to 42.5% of the control. Moreover, this
strain also improved the growth parameters of the rice seedlings significantly by increasing the shoot length, root
length, fresh and dry weight by 166.7%, 168.2%, 100%, and 255.6%; respectively, compared to the positive
control (Xoo only). Three antimicrobial related genes; pmxB, pmxD, and Fusdel were detected in P. polymyxa
SX3 using nine specific gene markers. The aims of the study were to investigate the impact of P. polymyxa SX3
on in vitro growth of Xoo, in vivo growth of the rice seedlings in the greenhouse, and to detect the presence of
related antimicrobial genes. |
| first_indexed | 2024-04-13T08:12:36Z |
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| institution | Directory Open Access Journal |
| issn | 2537-0286 2537-0294 |
| language | English |
| last_indexed | 2024-04-24T08:32:28Z |
| publishDate | 2020-08-01 |
| publisher | The National Information and Documentation Centre (NIDOC) , Egypt |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj.art-81becc7675b14eada4e5fbdabbe169d22024-04-16T19:04:52ZengThe National Information and Documentation Centre (NIDOC) , EgyptNovel Research in Microbiology Journal2537-02862537-02942020-08-014493995410.21608/nrmj.2020.107545Detection of the antibacterial efficacy of Paenibacillus polymyxa against Xanthomonas oryzae pv. oryzae in rice seedlings, and the antimicrobial related genesYasmine Abdallah0Ranya M. El-Ashmony1Abdelrazek S. Abdelrhim2Department of Plant Pathology, Faculty of Agriculture, Minia University, El-Minia, 11432, EgyptDepartment of Plant Pathology, Faculty of Agriculture, Minia University, El-Minia, 11432, EgyptDepartment of Plant Pathology, Faculty of Agriculture, Minia University, El-Minia, 11432, EgyptPaenibacillus polymyxa bacterium has been known as a biocontrol agent (BCA) for various plant diseases, and it promotes the growth of several treated plants. On the other hand, Xanthomonas oryzae pv. oryzae (Xoo) is an important bacterial pathogen that causes bacterial leaf blight (BLB) to rice plants, and cause high yield loss during the growing season. In this study, P. polymyxa SX3 inhibited the in vitro growth of Xoo by 80% and affected the biofilm formation. Also, the cell membrane of Xoo lost its permeability after treating it with culture filtrate of P. polymyxa at its MIC (40%). Transmission electron microscopic (TEM) observations showed an influence of the culture filtrate of P. polymyxa on the cell morphology of Xoo. Applying P. polymyxa decreased the severity of bacterial leaf blight on rice seedlings to 4.9%, compared to 42.5% of the control. Moreover, this strain also improved the growth parameters of the rice seedlings significantly by increasing the shoot length, root length, fresh and dry weight by 166.7%, 168.2%, 100%, and 255.6%; respectively, compared to the positive control (Xoo only). Three antimicrobial related genes; pmxB, pmxD, and Fusdel were detected in P. polymyxa SX3 using nine specific gene markers. The aims of the study were to investigate the impact of P. polymyxa SX3 on in vitro growth of Xoo, in vivo growth of the rice seedlings in the greenhouse, and to detect the presence of related antimicrobial genes.https://nrmj.journals.ekb.eg/article_107545_202ea527eb3f1c09f5522bd0250ddfff.pdfpaenibacillus polymyxaxanthomonas oryzae pv. oryazaebiocontrolantimicrobial genesrice |
| spellingShingle | Yasmine Abdallah Ranya M. El-Ashmony Abdelrazek S. Abdelrhim Detection of the antibacterial efficacy of Paenibacillus polymyxa against Xanthomonas oryzae pv. oryzae in rice seedlings, and the antimicrobial related genes Novel Research in Microbiology Journal paenibacillus polymyxa xanthomonas oryzae pv. oryazae biocontrol antimicrobial genes rice |
| title | Detection of the antibacterial efficacy of Paenibacillus polymyxa against Xanthomonas oryzae pv. oryzae in rice seedlings, and the antimicrobial related genes |
| title_full | Detection of the antibacterial efficacy of Paenibacillus polymyxa against Xanthomonas oryzae pv. oryzae in rice seedlings, and the antimicrobial related genes |
| title_fullStr | Detection of the antibacterial efficacy of Paenibacillus polymyxa against Xanthomonas oryzae pv. oryzae in rice seedlings, and the antimicrobial related genes |
| title_full_unstemmed | Detection of the antibacterial efficacy of Paenibacillus polymyxa against Xanthomonas oryzae pv. oryzae in rice seedlings, and the antimicrobial related genes |
| title_short | Detection of the antibacterial efficacy of Paenibacillus polymyxa against Xanthomonas oryzae pv. oryzae in rice seedlings, and the antimicrobial related genes |
| title_sort | detection of the antibacterial efficacy of paenibacillus polymyxa against xanthomonas oryzae pv oryzae in rice seedlings and the antimicrobial related genes |
| topic | paenibacillus polymyxa xanthomonas oryzae pv. oryazae biocontrol antimicrobial genes rice |
| url | https://nrmj.journals.ekb.eg/article_107545_202ea527eb3f1c09f5522bd0250ddfff.pdf |
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