Human vascular endothelial cells express epithelial growth factor in response to infection by Bartonella bacilliformis.
Bartonella are Gram-negative bacterial pathogens that trigger pathological angiogenesis during infection of humans. Bartonella bacilliformis (Bb) is a neglected tropical agent endemic to South America, where it causes Carrión's disease. Little is known about Bb's virulence determinants or...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-04-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0008236 |
| _version_ | 1797818576302768128 |
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| author | Linda D Hicks Michael F Minnick |
| author_facet | Linda D Hicks Michael F Minnick |
| author_sort | Linda D Hicks |
| collection | DOAJ |
| description | Bartonella are Gram-negative bacterial pathogens that trigger pathological angiogenesis during infection of humans. Bartonella bacilliformis (Bb) is a neglected tropical agent endemic to South America, where it causes Carrión's disease. Little is known about Bb's virulence determinants or how the pathogen elicits hyperproliferation of the vasculature, culminating in Peruvian warts (verruga peruana) of the skin. In this study, we determined that active infection of human umbilical vein endothelial cells (HUVECs) by live Bb induced host cell secretion of epidermal growth factor (EGF) using ELISA. Killed bacteria or lysates of various Bb strains did not cause EGF production, suggesting that an active infection was necessary for the response. Bb also caused hyperproliferation of infected HUVECs, and the mitogenic response could be inhibited by the EGF-receptor (EGFR) inhibitor, AG1478. Bb strains engineered to overexpress recombinant GroEL, evoked greater EGF production and hyperproliferation of HUVECs compared to control strains. Conditioned (spent) media from cultured HUVECs that had been previously infected by Bb were found to be mitogenic for naïve HUVECs, and the response could be inhibited by EGFR blocking with AG1478. Bb cells and cell lysates stimulated HUVEC migration and capillary-like tube formation in transmigration and Matrigel assays, respectively. To our knowledge, this is the first demonstration of EGF production by Bb-infected endothelial cells; an association that could contribute to hyperproliferation of the vascular bed during bartonellosis. |
| first_indexed | 2024-03-13T09:09:56Z |
| format | Article |
| id | doaj.art-81c07e790346437b9aa9388c83ae1713 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-03-13T09:09:56Z |
| publishDate | 2020-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-81c07e790346437b9aa9388c83ae17132023-05-27T05:31:58ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352020-04-01144e000823610.1371/journal.pntd.0008236Human vascular endothelial cells express epithelial growth factor in response to infection by Bartonella bacilliformis.Linda D HicksMichael F MinnickBartonella are Gram-negative bacterial pathogens that trigger pathological angiogenesis during infection of humans. Bartonella bacilliformis (Bb) is a neglected tropical agent endemic to South America, where it causes Carrión's disease. Little is known about Bb's virulence determinants or how the pathogen elicits hyperproliferation of the vasculature, culminating in Peruvian warts (verruga peruana) of the skin. In this study, we determined that active infection of human umbilical vein endothelial cells (HUVECs) by live Bb induced host cell secretion of epidermal growth factor (EGF) using ELISA. Killed bacteria or lysates of various Bb strains did not cause EGF production, suggesting that an active infection was necessary for the response. Bb also caused hyperproliferation of infected HUVECs, and the mitogenic response could be inhibited by the EGF-receptor (EGFR) inhibitor, AG1478. Bb strains engineered to overexpress recombinant GroEL, evoked greater EGF production and hyperproliferation of HUVECs compared to control strains. Conditioned (spent) media from cultured HUVECs that had been previously infected by Bb were found to be mitogenic for naïve HUVECs, and the response could be inhibited by EGFR blocking with AG1478. Bb cells and cell lysates stimulated HUVEC migration and capillary-like tube formation in transmigration and Matrigel assays, respectively. To our knowledge, this is the first demonstration of EGF production by Bb-infected endothelial cells; an association that could contribute to hyperproliferation of the vascular bed during bartonellosis.https://doi.org/10.1371/journal.pntd.0008236 |
| spellingShingle | Linda D Hicks Michael F Minnick Human vascular endothelial cells express epithelial growth factor in response to infection by Bartonella bacilliformis. PLoS Neglected Tropical Diseases |
| title | Human vascular endothelial cells express epithelial growth factor in response to infection by Bartonella bacilliformis. |
| title_full | Human vascular endothelial cells express epithelial growth factor in response to infection by Bartonella bacilliformis. |
| title_fullStr | Human vascular endothelial cells express epithelial growth factor in response to infection by Bartonella bacilliformis. |
| title_full_unstemmed | Human vascular endothelial cells express epithelial growth factor in response to infection by Bartonella bacilliformis. |
| title_short | Human vascular endothelial cells express epithelial growth factor in response to infection by Bartonella bacilliformis. |
| title_sort | human vascular endothelial cells express epithelial growth factor in response to infection by bartonella bacilliformis |
| url | https://doi.org/10.1371/journal.pntd.0008236 |
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