Molecular Characterization of Hard Ticks Infesting Camels in the Northern Region of Saudi Arabia Using the Barcoding Gene, Mitochondrial <i>Cytochrome oxidase subunit I</i>
The present study aimed to molecularly identify and characterize the hard ticks infesting camels from the northern region (Ha’il province) of Saudi Arabia using the mitochondrial barcoding gene <i>cytochrome oxidase subunit I</i> (<i>COI</i>). The sequences of tick samples fr...
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MDPI AG
2023-07-01
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| author | Fevzi Bardakci Sarah Hilan Mohammed Al-Subaie Riadh Badraoui Mohd Adnan Arif Jamal Siddiqui |
| author_facet | Fevzi Bardakci Sarah Hilan Mohammed Al-Subaie Riadh Badraoui Mohd Adnan Arif Jamal Siddiqui |
| author_sort | Fevzi Bardakci |
| collection | DOAJ |
| description | The present study aimed to molecularly identify and characterize the hard ticks infesting camels from the northern region (Ha’il province) of Saudi Arabia using the mitochondrial barcoding gene <i>cytochrome oxidase subunit I</i> (<i>COI</i>). The sequences of tick samples from camels in three regions of Ha’il were aligned with those previously reported from different geographic regions, revealing nine haplotypes, of which six were newly described in this study for the first time. These haplotypes were used to determine their phylogenetic relationships using the maximum likelihood method, displaying two distinct clades corresponding to <i>Hyalomma dromedarii</i> and <i>H. impeltatum</i>. Moreover, the haplotypes showing the highest homology with those deposited in NCBI-GenBank from different geographic regions, including Saudi Arabia, were obtained and combined to determine their phylogenetic relationships among them. The results showed that the haplotypes belonging to two clades were grouped with those previously determined as <i>H. dromedarii</i> and <i>H. impeltatum</i>. Moreover, the presence of <i>H. scupense</i> (syn. <i>H. detritum</i>) together with <i>H. impeltatum</i> suggests possible asymmetrical hybridization and mitochondrial introgression between these species. <i>H. scupense</i> infesting different mammal species apart from camels were also clustered in a different clade, indicating the presence of different lineages of this species that show different host specificities. |
| first_indexed | 2024-03-11T00:53:56Z |
| format | Article |
| id | doaj.art-81c08815a63c493dac9fbad5522f9a83 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-11T00:53:56Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-81c08815a63c493dac9fbad5522f9a832023-11-18T20:09:51ZengMDPI AGLife2075-17292023-07-01137153510.3390/life13071535Molecular Characterization of Hard Ticks Infesting Camels in the Northern Region of Saudi Arabia Using the Barcoding Gene, Mitochondrial <i>Cytochrome oxidase subunit I</i>Fevzi Bardakci0Sarah Hilan Mohammed Al-Subaie1Riadh Badraoui2Mohd Adnan3Arif Jamal Siddiqui4Department of Biology, College of Science, University of Ha’il, Ha’il P.O. Box 2440, Saudi ArabiaDepartment of Biology, College of Science, University of Ha’il, Ha’il P.O. Box 2440, Saudi ArabiaSection of Histology-Cytology, Medicine Faculty of Tunis, University of Tunis El Manar, Tunis 1007, TunisiaDepartment of Biology, College of Science, University of Ha’il, Ha’il P.O. Box 2440, Saudi ArabiaDepartment of Biology, College of Science, University of Ha’il, Ha’il P.O. Box 2440, Saudi ArabiaThe present study aimed to molecularly identify and characterize the hard ticks infesting camels from the northern region (Ha’il province) of Saudi Arabia using the mitochondrial barcoding gene <i>cytochrome oxidase subunit I</i> (<i>COI</i>). The sequences of tick samples from camels in three regions of Ha’il were aligned with those previously reported from different geographic regions, revealing nine haplotypes, of which six were newly described in this study for the first time. These haplotypes were used to determine their phylogenetic relationships using the maximum likelihood method, displaying two distinct clades corresponding to <i>Hyalomma dromedarii</i> and <i>H. impeltatum</i>. Moreover, the haplotypes showing the highest homology with those deposited in NCBI-GenBank from different geographic regions, including Saudi Arabia, were obtained and combined to determine their phylogenetic relationships among them. The results showed that the haplotypes belonging to two clades were grouped with those previously determined as <i>H. dromedarii</i> and <i>H. impeltatum</i>. Moreover, the presence of <i>H. scupense</i> (syn. <i>H. detritum</i>) together with <i>H. impeltatum</i> suggests possible asymmetrical hybridization and mitochondrial introgression between these species. <i>H. scupense</i> infesting different mammal species apart from camels were also clustered in a different clade, indicating the presence of different lineages of this species that show different host specificities.https://www.mdpi.com/2075-1729/13/7/1535hard ticks<i>cytochrome oxidase subunit I</i><i>COI</i>phylogeneticspopulation genetics<i>Hyalloma</i> |
| spellingShingle | Fevzi Bardakci Sarah Hilan Mohammed Al-Subaie Riadh Badraoui Mohd Adnan Arif Jamal Siddiqui Molecular Characterization of Hard Ticks Infesting Camels in the Northern Region of Saudi Arabia Using the Barcoding Gene, Mitochondrial <i>Cytochrome oxidase subunit I</i> Life hard ticks <i>cytochrome oxidase subunit I</i> <i>COI</i> phylogenetics population genetics <i>Hyalloma</i> |
| title | Molecular Characterization of Hard Ticks Infesting Camels in the Northern Region of Saudi Arabia Using the Barcoding Gene, Mitochondrial <i>Cytochrome oxidase subunit I</i> |
| title_full | Molecular Characterization of Hard Ticks Infesting Camels in the Northern Region of Saudi Arabia Using the Barcoding Gene, Mitochondrial <i>Cytochrome oxidase subunit I</i> |
| title_fullStr | Molecular Characterization of Hard Ticks Infesting Camels in the Northern Region of Saudi Arabia Using the Barcoding Gene, Mitochondrial <i>Cytochrome oxidase subunit I</i> |
| title_full_unstemmed | Molecular Characterization of Hard Ticks Infesting Camels in the Northern Region of Saudi Arabia Using the Barcoding Gene, Mitochondrial <i>Cytochrome oxidase subunit I</i> |
| title_short | Molecular Characterization of Hard Ticks Infesting Camels in the Northern Region of Saudi Arabia Using the Barcoding Gene, Mitochondrial <i>Cytochrome oxidase subunit I</i> |
| title_sort | molecular characterization of hard ticks infesting camels in the northern region of saudi arabia using the barcoding gene mitochondrial i cytochrome oxidase subunit i i |
| topic | hard ticks <i>cytochrome oxidase subunit I</i> <i>COI</i> phylogenetics population genetics <i>Hyalloma</i> |
| url | https://www.mdpi.com/2075-1729/13/7/1535 |
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