Discrete Regions of the Sensor Protein VirA Determine the Strain-Specific Ability of Agrobacterium to Agroinfect Maize
The ability of Agrobacterium strains to infect transformation-recalcitrant maize plants has been shown to be determined mainly by the virA locus, implicating vir gene induction as the major factor influencing maize infection. In this report, we further explore the roles of vir induction-associated b...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
The American Phytopathological Society
1997-03-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1997.10.2.221 |
| _version_ | 1818757344371146752 |
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| author | J. D. Heath M. I. Boulton D. M. Raineri S. L. Doty A. R. Mushegian T. C. Charles J. W. Davies E. W. Nester |
| author_facet | J. D. Heath M. I. Boulton D. M. Raineri S. L. Doty A. R. Mushegian T. C. Charles J. W. Davies E. W. Nester |
| author_sort | J. D. Heath |
| collection | DOAJ |
| description | The ability of Agrobacterium strains to infect transformation-recalcitrant maize plants has been shown to be determined mainly by the virA locus, implicating vir gene induction as the major factor influencing maize infection. In this report, we further explore the roles of vir induction-associated bacterial factors in maize infection using the technique of agroinfection. The Ti plasmid and virA source are shown to be important in determining the ability of a strain to infect maize, and the monosaccharide binding protein ChvE is absolutely required for maize agroinfection. The linker domain of VirAC58 from an agroinfection-competent strain, C58, is sufficient to convert VirAA6 of a nonagroinfecting strain, A348, to agroinfection competence. The periplasmic domain of VirAC58 is also able to confer a moderate level of agroinfection competence to VirAA6. In addition, the VirAA6 protein from A348 is agroinfection competent when removed from its cognate Ti plasmid background and placed in a pTiC58 background. The presence of a pTiA6-encoded, VirAA6-specific inhibitor is hypothesized and examined. |
| first_indexed | 2024-12-18T06:09:27Z |
| format | Article |
| id | doaj.art-81c41537598b4314bbb97c9de133efe5 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-18T06:09:27Z |
| publishDate | 1997-03-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-81c41537598b4314bbb97c9de133efe52022-12-21T21:18:27ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061997-03-0110222122710.1094/MPMI.1997.10.2.221Discrete Regions of the Sensor Protein VirA Determine the Strain-Specific Ability of Agrobacterium to Agroinfect MaizeJ. D. HeathM. I. BoultonD. M. RaineriS. L. DotyA. R. MushegianT. C. CharlesJ. W. DaviesE. W. NesterThe ability of Agrobacterium strains to infect transformation-recalcitrant maize plants has been shown to be determined mainly by the virA locus, implicating vir gene induction as the major factor influencing maize infection. In this report, we further explore the roles of vir induction-associated bacterial factors in maize infection using the technique of agroinfection. The Ti plasmid and virA source are shown to be important in determining the ability of a strain to infect maize, and the monosaccharide binding protein ChvE is absolutely required for maize agroinfection. The linker domain of VirAC58 from an agroinfection-competent strain, C58, is sufficient to convert VirAA6 of a nonagroinfecting strain, A348, to agroinfection competence. The periplasmic domain of VirAC58 is also able to confer a moderate level of agroinfection competence to VirAA6. In addition, the VirAA6 protein from A348 is agroinfection competent when removed from its cognate Ti plasmid background and placed in a pTiC58 background. The presence of a pTiA6-encoded, VirAA6-specific inhibitor is hypothesized and examined.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1997.10.2.221host rangeplant transformationtwo-component system |
| spellingShingle | J. D. Heath M. I. Boulton D. M. Raineri S. L. Doty A. R. Mushegian T. C. Charles J. W. Davies E. W. Nester Discrete Regions of the Sensor Protein VirA Determine the Strain-Specific Ability of Agrobacterium to Agroinfect Maize Molecular Plant-Microbe Interactions host range plant transformation two-component system |
| title | Discrete Regions of the Sensor Protein VirA Determine the Strain-Specific Ability of Agrobacterium to Agroinfect Maize |
| title_full | Discrete Regions of the Sensor Protein VirA Determine the Strain-Specific Ability of Agrobacterium to Agroinfect Maize |
| title_fullStr | Discrete Regions of the Sensor Protein VirA Determine the Strain-Specific Ability of Agrobacterium to Agroinfect Maize |
| title_full_unstemmed | Discrete Regions of the Sensor Protein VirA Determine the Strain-Specific Ability of Agrobacterium to Agroinfect Maize |
| title_short | Discrete Regions of the Sensor Protein VirA Determine the Strain-Specific Ability of Agrobacterium to Agroinfect Maize |
| title_sort | discrete regions of the sensor protein vira determine the strain specific ability of agrobacterium to agroinfect maize |
| topic | host range plant transformation two-component system |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1997.10.2.221 |
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