Differences in Genotype and Antimicrobial Resistance between <i>Campylobacter</i> spp. Isolated from Organic and Conventionally Produced Chickens in Sweden
Antibiotic resistance is a major challenge worldwide and increased resistance to quinolones in <i>Campylobacter</i> is being reported. Analysis of antibiotic resistance was performed on 157 <i>Campylobacter</i> strains (123 <i>C. jejuni</i> and 34 <i>C. coli...
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MDPI AG
2021-12-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/12/1630 |
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| author | Ingrid Hansson Patrik Ellström Oskar Nilsson Matilda Chaba Moa Skarin Lise-Lotte Fernström Sara Frosth |
| author_facet | Ingrid Hansson Patrik Ellström Oskar Nilsson Matilda Chaba Moa Skarin Lise-Lotte Fernström Sara Frosth |
| author_sort | Ingrid Hansson |
| collection | DOAJ |
| description | Antibiotic resistance is a major challenge worldwide and increased resistance to quinolones in <i>Campylobacter</i> is being reported. Analysis of antibiotic resistance was performed on 157 <i>Campylobacter</i> strains (123 <i>C. jejuni</i> and 34 <i>C. coli</i>) from conventional and organic chickens produced in Sweden. Susceptibility for tetracycline, ciprofloxacin, erythromycin, nalidixic acid, streptomycin, and gentamycin was determined by microdilution. All 77 isolates from organic chickens were sensitive to all antibiotics, except two <i>C. jejuni</i> that were resistant to tetracycline. Of the 80 isolates from conventional chickens, 22.5% of <i>C. jejuni</i> and 11.1% of <i>C. coli</i> were resistant to quinolones and 5.6% of <i>C. jejuni</i> were resistant to tetracycline. Whole-genome sequencing resulted in 50 different sequence types of <i>C. jejuni</i> and six of <i>C. coli.</i> Nine sequence types were found in both organic and conventional chickens. Two of these (ST-19 and ST-257) included isolates from conventional broilers with different resistance phenotypes to the remaining isolates from conventional and organic broilers. There are management differences between the production systems, such as feed, breed, use of coccidiostats, and access to outdoor area. It is unlikely that quinolone resistance has arisen due to use of antimicrobials, since fluoroquinolones are not permitted in Swedish broiler production. |
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| language | English |
| last_indexed | 2024-03-10T03:21:32Z |
| publishDate | 2021-12-01 |
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| series | Pathogens |
| spelling | doaj.art-81c4e20f90bf4e2c9ae4b8b0832529c12023-11-23T10:01:45ZengMDPI AGPathogens2076-08172021-12-011012163010.3390/pathogens10121630Differences in Genotype and Antimicrobial Resistance between <i>Campylobacter</i> spp. Isolated from Organic and Conventionally Produced Chickens in SwedenIngrid Hansson0Patrik Ellström1Oskar Nilsson2Matilda Chaba3Moa Skarin4Lise-Lotte Fernström5Sara Frosth6Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, P.O. Box 7036, SE-750 07 Uppsala, SwedenDepartment of Medical Sciences, Uppsala University, SE-751 85 Uppsala, SwedenDepartment of Animal Health and Antimicrobial Strategies, National Veterinary Institute (SVA), SE-751 89 Uppsala, SwedenDepartment of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, P.O. Box 7036, SE-750 07 Uppsala, SwedenDepartment of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, P.O. Box 7036, SE-750 07 Uppsala, SwedenDepartment of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, P.O. Box 7036, SE-750 07 Uppsala, SwedenDepartment of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, P.O. Box 7036, SE-750 07 Uppsala, SwedenAntibiotic resistance is a major challenge worldwide and increased resistance to quinolones in <i>Campylobacter</i> is being reported. Analysis of antibiotic resistance was performed on 157 <i>Campylobacter</i> strains (123 <i>C. jejuni</i> and 34 <i>C. coli</i>) from conventional and organic chickens produced in Sweden. Susceptibility for tetracycline, ciprofloxacin, erythromycin, nalidixic acid, streptomycin, and gentamycin was determined by microdilution. All 77 isolates from organic chickens were sensitive to all antibiotics, except two <i>C. jejuni</i> that were resistant to tetracycline. Of the 80 isolates from conventional chickens, 22.5% of <i>C. jejuni</i> and 11.1% of <i>C. coli</i> were resistant to quinolones and 5.6% of <i>C. jejuni</i> were resistant to tetracycline. Whole-genome sequencing resulted in 50 different sequence types of <i>C. jejuni</i> and six of <i>C. coli.</i> Nine sequence types were found in both organic and conventional chickens. Two of these (ST-19 and ST-257) included isolates from conventional broilers with different resistance phenotypes to the remaining isolates from conventional and organic broilers. There are management differences between the production systems, such as feed, breed, use of coccidiostats, and access to outdoor area. It is unlikely that quinolone resistance has arisen due to use of antimicrobials, since fluoroquinolones are not permitted in Swedish broiler production.https://www.mdpi.com/2076-0817/10/12/1630antimicrobial resistancebroiler<i>Campylobacter jejuni</i><i>Campylobacter coli</i>organiccgMLST |
| spellingShingle | Ingrid Hansson Patrik Ellström Oskar Nilsson Matilda Chaba Moa Skarin Lise-Lotte Fernström Sara Frosth Differences in Genotype and Antimicrobial Resistance between <i>Campylobacter</i> spp. Isolated from Organic and Conventionally Produced Chickens in Sweden Pathogens antimicrobial resistance broiler <i>Campylobacter jejuni</i> <i>Campylobacter coli</i> organic cgMLST |
| title | Differences in Genotype and Antimicrobial Resistance between <i>Campylobacter</i> spp. Isolated from Organic and Conventionally Produced Chickens in Sweden |
| title_full | Differences in Genotype and Antimicrobial Resistance between <i>Campylobacter</i> spp. Isolated from Organic and Conventionally Produced Chickens in Sweden |
| title_fullStr | Differences in Genotype and Antimicrobial Resistance between <i>Campylobacter</i> spp. Isolated from Organic and Conventionally Produced Chickens in Sweden |
| title_full_unstemmed | Differences in Genotype and Antimicrobial Resistance between <i>Campylobacter</i> spp. Isolated from Organic and Conventionally Produced Chickens in Sweden |
| title_short | Differences in Genotype and Antimicrobial Resistance between <i>Campylobacter</i> spp. Isolated from Organic and Conventionally Produced Chickens in Sweden |
| title_sort | differences in genotype and antimicrobial resistance between i campylobacter i spp isolated from organic and conventionally produced chickens in sweden |
| topic | antimicrobial resistance broiler <i>Campylobacter jejuni</i> <i>Campylobacter coli</i> organic cgMLST |
| url | https://www.mdpi.com/2076-0817/10/12/1630 |
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