The Morphological Parameters and Cytosolic pH of Cells of Root Zones in Tobacco Plants (<i>Nicotiana tabacum</i> L.): Nonlinear Effects of NaCl Concentrations
Salinity impacts important processes in plants, reducing their yield. The effect of salinity on the cytosolic pH (pHcyt) has been little studied. In this research, we employed transgenic tobacco plants expressing the pH sensor Pt-GFP to investigate the alterations in pHcyt in cells across various ro...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/21/3708 |
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| author | Maria N. Ageyeva Tatiana A. Zdobnova Mariia S. Nazarova Galina N. Raldugina Denis V. Beliaev Vladimir A. Vodeneev Anna A. Brilkina |
| author_facet | Maria N. Ageyeva Tatiana A. Zdobnova Mariia S. Nazarova Galina N. Raldugina Denis V. Beliaev Vladimir A. Vodeneev Anna A. Brilkina |
| author_sort | Maria N. Ageyeva |
| collection | DOAJ |
| description | Salinity impacts important processes in plants, reducing their yield. The effect of salinity on the cytosolic pH (pHcyt) has been little studied. In this research, we employed transgenic tobacco plants expressing the pH sensor Pt-GFP to investigate the alterations in pHcyt in cells across various root zones. Furthermore, we examined a wide spectrum of NaCl concentrations (ranging from 0 to 150 mM) and assessed morphological parameters and plant development. Our findings revealed a pattern of cytosolic acidification in cells across all root zones at lower NaCl concentrations (50, 100 mM). Interestingly, at 150 mM NaCl, pHcyt levels either increased or returned to normal, indicating a nonlinear effect of salinity on pHcyt. Most studied parameters related to development and morphology exhibited an inhibitory influence in response to NaCl. Notably, a nonlinear relationship was observed in the cell length within the elongation and differentiation zones. While cell elongation occurred at 50 and 100 mM NaCl, it was not evident at 150 mM NaCl. This suggests a complex interplay between stimulating and inhibitory effects of salinity, contributing to the nonlinear relationship observed between pHcyt, cell length, and NaCl concentration. |
| first_indexed | 2024-03-11T11:23:30Z |
| format | Article |
| id | doaj.art-81c7975104904542aed4938041e7550c |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T11:23:30Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-81c7975104904542aed4938041e7550c2023-11-10T15:10:15ZengMDPI AGPlants2223-77472023-10-011221370810.3390/plants12213708The Morphological Parameters and Cytosolic pH of Cells of Root Zones in Tobacco Plants (<i>Nicotiana tabacum</i> L.): Nonlinear Effects of NaCl ConcentrationsMaria N. Ageyeva0Tatiana A. Zdobnova1Mariia S. Nazarova2Galina N. Raldugina3Denis V. Beliaev4Vladimir A. Vodeneev5Anna A. Brilkina6Department of Biochemistry and Biotechnology, National Research Lobachevsky State University of Nizhny Novgorod, 603950 Nizhny Novgorod, RussiaDepartment of Biophysics, National Research Lobachevsky State University of Nizhny Novgorod, 603950 Nizhny Novgorod, RussiaDepartment of Biochemistry and Biotechnology, National Research Lobachevsky State University of Nizhny Novgorod, 603950 Nizhny Novgorod, RussiaLaboratory of Ion Transport and Salinity Resistance, K. A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276 Moscow, RussiaMoscow Institute of Physics and Technology, 141700 Dolgoprudny, RussiaDepartment of Biophysics, National Research Lobachevsky State University of Nizhny Novgorod, 603950 Nizhny Novgorod, RussiaDepartment of Biochemistry and Biotechnology, National Research Lobachevsky State University of Nizhny Novgorod, 603950 Nizhny Novgorod, RussiaSalinity impacts important processes in plants, reducing their yield. The effect of salinity on the cytosolic pH (pHcyt) has been little studied. In this research, we employed transgenic tobacco plants expressing the pH sensor Pt-GFP to investigate the alterations in pHcyt in cells across various root zones. Furthermore, we examined a wide spectrum of NaCl concentrations (ranging from 0 to 150 mM) and assessed morphological parameters and plant development. Our findings revealed a pattern of cytosolic acidification in cells across all root zones at lower NaCl concentrations (50, 100 mM). Interestingly, at 150 mM NaCl, pHcyt levels either increased or returned to normal, indicating a nonlinear effect of salinity on pHcyt. Most studied parameters related to development and morphology exhibited an inhibitory influence in response to NaCl. Notably, a nonlinear relationship was observed in the cell length within the elongation and differentiation zones. While cell elongation occurred at 50 and 100 mM NaCl, it was not evident at 150 mM NaCl. This suggests a complex interplay between stimulating and inhibitory effects of salinity, contributing to the nonlinear relationship observed between pHcyt, cell length, and NaCl concentration.https://www.mdpi.com/2223-7747/12/21/3708salinityroot zonesroot capcell lengthplant developmentpH sensor calibration |
| spellingShingle | Maria N. Ageyeva Tatiana A. Zdobnova Mariia S. Nazarova Galina N. Raldugina Denis V. Beliaev Vladimir A. Vodeneev Anna A. Brilkina The Morphological Parameters and Cytosolic pH of Cells of Root Zones in Tobacco Plants (<i>Nicotiana tabacum</i> L.): Nonlinear Effects of NaCl Concentrations Plants salinity root zones root cap cell length plant development pH sensor calibration |
| title | The Morphological Parameters and Cytosolic pH of Cells of Root Zones in Tobacco Plants (<i>Nicotiana tabacum</i> L.): Nonlinear Effects of NaCl Concentrations |
| title_full | The Morphological Parameters and Cytosolic pH of Cells of Root Zones in Tobacco Plants (<i>Nicotiana tabacum</i> L.): Nonlinear Effects of NaCl Concentrations |
| title_fullStr | The Morphological Parameters and Cytosolic pH of Cells of Root Zones in Tobacco Plants (<i>Nicotiana tabacum</i> L.): Nonlinear Effects of NaCl Concentrations |
| title_full_unstemmed | The Morphological Parameters and Cytosolic pH of Cells of Root Zones in Tobacco Plants (<i>Nicotiana tabacum</i> L.): Nonlinear Effects of NaCl Concentrations |
| title_short | The Morphological Parameters and Cytosolic pH of Cells of Root Zones in Tobacco Plants (<i>Nicotiana tabacum</i> L.): Nonlinear Effects of NaCl Concentrations |
| title_sort | morphological parameters and cytosolic ph of cells of root zones in tobacco plants i nicotiana tabacum i l nonlinear effects of nacl concentrations |
| topic | salinity root zones root cap cell length plant development pH sensor calibration |
| url | https://www.mdpi.com/2223-7747/12/21/3708 |
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