Use of Secondary Metabolites Profiling and Antioxidant Activity to Unravel the Differences between Two Species of Nettle
In recent years, the interest in natural remedies has increased, so it is important to analyze the plants widely distributed in nature but whose composition is little known. The main objective of the present work is to obtain information based on the profiles of secondary metabolites and antioxidant...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/18/3233 |
| _version_ | 1797577824071057408 |
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| author | Julia Baumli Norbert Antal Dorina Casoni Claudia Cimpoiu |
| author_facet | Julia Baumli Norbert Antal Dorina Casoni Claudia Cimpoiu |
| author_sort | Julia Baumli |
| collection | DOAJ |
| description | In recent years, the interest in natural remedies has increased, so it is important to analyze the plants widely distributed in nature but whose composition is little known. The main objective of the present work is to obtain information based on the profiles of secondary metabolites and antioxidant activity in <i>Lamium album</i>, a very widespread but little studied plant, with the aim of revealing the differences compared to <i>Urtica dioica</i>. First, the optimization of enzymatic extraction assisted by ultrasound was carried out by the Box–Behnken method. The optimized parameters were: concentration of the enzyme—3.3% cellulase, temperature—55 °C, and the extraction time—40.00 min. The efficiency was estimated based on the content of iridoids, the main class of secondary metabolites from <i>Lamium album.</i> Second, the secondary metabolites profiles of the nettle extracts were obtained by thin-layer chromatography using both normal and reverse phases and by RP-UHPLC. The antioxidant activity was evaluated using DPPH and ABTS<sup>+</sup> radicals. The obtained results revealed significant differences between the two nettle species, both in terms of the phytochemical compounds, as well as the antioxidant activity, confirming the fact that <i>Lamium album</i> has a high potential to be used in phytomedicine. |
| first_indexed | 2024-03-10T22:13:26Z |
| format | Article |
| id | doaj.art-81c7a170fc35469eb69bf88cbfbf98ff |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T22:13:26Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-81c7a170fc35469eb69bf88cbfbf98ff2023-11-19T12:31:57ZengMDPI AGPlants2223-77472023-09-011218323310.3390/plants12183233Use of Secondary Metabolites Profiling and Antioxidant Activity to Unravel the Differences between Two Species of NettleJulia Baumli0Norbert Antal1Dorina Casoni2Claudia Cimpoiu3Faculty of Chemistry and Chemical Engineering, Babes-Bolyai University, 11 Arany Janos, 400028 Cluj-Napoca, RomaniaFaculty of Chemistry and Chemical Engineering, Babes-Bolyai University, 11 Arany Janos, 400028 Cluj-Napoca, RomaniaFaculty of Chemistry and Chemical Engineering, Babes-Bolyai University, 11 Arany Janos, 400028 Cluj-Napoca, RomaniaFaculty of Chemistry and Chemical Engineering, Babes-Bolyai University, 11 Arany Janos, 400028 Cluj-Napoca, RomaniaIn recent years, the interest in natural remedies has increased, so it is important to analyze the plants widely distributed in nature but whose composition is little known. The main objective of the present work is to obtain information based on the profiles of secondary metabolites and antioxidant activity in <i>Lamium album</i>, a very widespread but little studied plant, with the aim of revealing the differences compared to <i>Urtica dioica</i>. First, the optimization of enzymatic extraction assisted by ultrasound was carried out by the Box–Behnken method. The optimized parameters were: concentration of the enzyme—3.3% cellulase, temperature—55 °C, and the extraction time—40.00 min. The efficiency was estimated based on the content of iridoids, the main class of secondary metabolites from <i>Lamium album.</i> Second, the secondary metabolites profiles of the nettle extracts were obtained by thin-layer chromatography using both normal and reverse phases and by RP-UHPLC. The antioxidant activity was evaluated using DPPH and ABTS<sup>+</sup> radicals. The obtained results revealed significant differences between the two nettle species, both in terms of the phytochemical compounds, as well as the antioxidant activity, confirming the fact that <i>Lamium album</i> has a high potential to be used in phytomedicine.https://www.mdpi.com/2223-7747/12/18/3233extraction optimizationBox–Behnken methodiridoidssecondary metabolites profilingantioxidant activitythin-layer chromatography |
| spellingShingle | Julia Baumli Norbert Antal Dorina Casoni Claudia Cimpoiu Use of Secondary Metabolites Profiling and Antioxidant Activity to Unravel the Differences between Two Species of Nettle Plants extraction optimization Box–Behnken method iridoids secondary metabolites profiling antioxidant activity thin-layer chromatography |
| title | Use of Secondary Metabolites Profiling and Antioxidant Activity to Unravel the Differences between Two Species of Nettle |
| title_full | Use of Secondary Metabolites Profiling and Antioxidant Activity to Unravel the Differences between Two Species of Nettle |
| title_fullStr | Use of Secondary Metabolites Profiling and Antioxidant Activity to Unravel the Differences between Two Species of Nettle |
| title_full_unstemmed | Use of Secondary Metabolites Profiling and Antioxidant Activity to Unravel the Differences between Two Species of Nettle |
| title_short | Use of Secondary Metabolites Profiling and Antioxidant Activity to Unravel the Differences between Two Species of Nettle |
| title_sort | use of secondary metabolites profiling and antioxidant activity to unravel the differences between two species of nettle |
| topic | extraction optimization Box–Behnken method iridoids secondary metabolites profiling antioxidant activity thin-layer chromatography |
| url | https://www.mdpi.com/2223-7747/12/18/3233 |
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