Harnessing the Potential of Halogenated Natural Product Biosynthesis by Mangrove-Derived Actinomycetes
Mangrove-derived actinomycetes are promising sources of bioactive natural products. In this study, using homologous screening of the biosynthetic genes and anti-microorganism/tumor assaying, 163 strains of actinomycetes isolated from mangrove sediments were investigated for their potential to produc...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2013-10-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/11/10/3875 |
| _version_ | 1818001854047453184 |
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| author | Xiang Xiao Jun Xu Min-Juan Xu Shu-Jie Xie Li Xu Guang-Hui Ma Xiao-Min Tang Jing Xiao Xue-Gong Li |
| author_facet | Xiang Xiao Jun Xu Min-Juan Xu Shu-Jie Xie Li Xu Guang-Hui Ma Xiao-Min Tang Jing Xiao Xue-Gong Li |
| author_sort | Xiang Xiao |
| collection | DOAJ |
| description | Mangrove-derived actinomycetes are promising sources of bioactive natural products. In this study, using homologous screening of the biosynthetic genes and anti-microorganism/tumor assaying, 163 strains of actinomycetes isolated from mangrove sediments were investigated for their potential to produce halogenated metabolites. The FADH2-dependent halogenase genes, identified in PCR-screening, were clustered in distinct clades in the phylogenetic analysis. The coexistence of either polyketide synthase (PKS) or nonribosomal peptide synthetase (NRPS) as the backbone synthetases in the strains harboring the halogenase indicated that these strains had the potential to produce structurally diversified antibiotics. As a validation, a new enduracidin producer, Streptomyces atrovirens MGR140, was identified and confirmed by gene disruption and HPLC analysis. Moreover, a putative ansamycin biosynthesis gene cluster was detected in Streptomyces albogriseolus MGR072. Our results highlight that combined genome mining is an efficient technique to tap promising sources of halogenated natural products synthesized by mangrove-derived actinomycetes. |
| first_indexed | 2024-04-14T03:39:37Z |
| format | Article |
| id | doaj.art-81c8d0d29f57443f8532e5fca54db238 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-14T03:39:37Z |
| publishDate | 2013-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-81c8d0d29f57443f8532e5fca54db2382022-12-22T02:14:36ZengMDPI AGMarine Drugs1660-33972013-10-0111103875389010.3390/md11103875Harnessing the Potential of Halogenated Natural Product Biosynthesis by Mangrove-Derived ActinomycetesXiang XiaoJun XuMin-Juan XuShu-Jie XieLi XuGuang-Hui MaXiao-Min TangJing XiaoXue-Gong LiMangrove-derived actinomycetes are promising sources of bioactive natural products. In this study, using homologous screening of the biosynthetic genes and anti-microorganism/tumor assaying, 163 strains of actinomycetes isolated from mangrove sediments were investigated for their potential to produce halogenated metabolites. The FADH2-dependent halogenase genes, identified in PCR-screening, were clustered in distinct clades in the phylogenetic analysis. The coexistence of either polyketide synthase (PKS) or nonribosomal peptide synthetase (NRPS) as the backbone synthetases in the strains harboring the halogenase indicated that these strains had the potential to produce structurally diversified antibiotics. As a validation, a new enduracidin producer, Streptomyces atrovirens MGR140, was identified and confirmed by gene disruption and HPLC analysis. Moreover, a putative ansamycin biosynthesis gene cluster was detected in Streptomyces albogriseolus MGR072. Our results highlight that combined genome mining is an efficient technique to tap promising sources of halogenated natural products synthesized by mangrove-derived actinomycetes.http://www.mdpi.com/1660-3397/11/10/3875mangrove-derived actinomycetesgenome mininghalogenaseenduracidinansamycin |
| spellingShingle | Xiang Xiao Jun Xu Min-Juan Xu Shu-Jie Xie Li Xu Guang-Hui Ma Xiao-Min Tang Jing Xiao Xue-Gong Li Harnessing the Potential of Halogenated Natural Product Biosynthesis by Mangrove-Derived Actinomycetes Marine Drugs mangrove-derived actinomycetes genome mining halogenase enduracidin ansamycin |
| title | Harnessing the Potential of Halogenated Natural Product Biosynthesis by Mangrove-Derived Actinomycetes |
| title_full | Harnessing the Potential of Halogenated Natural Product Biosynthesis by Mangrove-Derived Actinomycetes |
| title_fullStr | Harnessing the Potential of Halogenated Natural Product Biosynthesis by Mangrove-Derived Actinomycetes |
| title_full_unstemmed | Harnessing the Potential of Halogenated Natural Product Biosynthesis by Mangrove-Derived Actinomycetes |
| title_short | Harnessing the Potential of Halogenated Natural Product Biosynthesis by Mangrove-Derived Actinomycetes |
| title_sort | harnessing the potential of halogenated natural product biosynthesis by mangrove derived actinomycetes |
| topic | mangrove-derived actinomycetes genome mining halogenase enduracidin ansamycin |
| url | http://www.mdpi.com/1660-3397/11/10/3875 |
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