Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10
Abstract Background This study aims to screen and identify the biological functions and prognostic value of CXC chemokines in ovarian cancer (OC) through bioinformatics and molecular biology methods, and to provide data support for the selection of biomarkers and prognostic analysis of OC. Methods I...
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| Format: | Article |
| Language: | English |
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BMC
2021-11-01
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| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12957-021-02440-x |
| _version_ | 1831548002282504192 |
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| author | Weiyuan Li Ji-Ao Ma Xun Sheng Chunjie Xiao |
| author_facet | Weiyuan Li Ji-Ao Ma Xun Sheng Chunjie Xiao |
| author_sort | Weiyuan Li |
| collection | DOAJ |
| description | Abstract Background This study aims to screen and identify the biological functions and prognostic value of CXC chemokines in ovarian cancer (OC) through bioinformatics and molecular biology methods, and to provide data support for the selection of biomarkers and prognostic analysis of OC. Methods In this study, GEO, ONCOMINE, GEPIA, cBioPortal, GeneMANIA, Metascape, STRING, TRRUST, and TIMER databases were used to study CXC chemokines. Angiogenesis and T cell killing assay were used to detect the effect of CXCL10 on tumor cell immunity and angiogenesis. Real-time quantitative PCR (qRT-PCR), immunoblotting, and ectopic tumor formation experiments were used to verify the effect of CXCL10 on ovarian cancer tumors. Results We found that CXCL1, CXCL10, CXCL11, CXCL13, and CXCL14 were significantly upregulated in OC samples compared with normal tissues. Our data showed that there was a relationship between the expression of CXC chemokines and the infiltration of six types of immune cells significant correlation. In vitro assay confirmed that overexpression of CXCL10 could enhance the killing effect of T cells and inhibit angiogenesis. Further in vivo assay had shown that CXCL10 could affect the progression of ovarian cancer by increasing the expression of cytotoxic T cells and inhibiting angiogenesis. Conclusion In conclusion, we hope that our data will provide new insights into the development of immunotherapy and the selection of prognostic markers for patients with OC. |
| first_indexed | 2024-12-17T02:11:22Z |
| format | Article |
| id | doaj.art-81c8d24183da4872ab79905e7b8ef450 |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-12-17T02:11:22Z |
| publishDate | 2021-11-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-81c8d24183da4872ab79905e7b8ef4502022-12-21T22:07:32ZengBMCWorld Journal of Surgical Oncology1477-78192021-11-0119111610.1186/s12957-021-02440-xScreening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10Weiyuan Li0Ji-Ao Ma1Xun Sheng2Chunjie Xiao3School of Medicine, Yunnan UniversitySchool of Medicine, Yunnan UniversitySchool of Medicine, Yunnan UniversitySchool of Medicine, Yunnan UniversityAbstract Background This study aims to screen and identify the biological functions and prognostic value of CXC chemokines in ovarian cancer (OC) through bioinformatics and molecular biology methods, and to provide data support for the selection of biomarkers and prognostic analysis of OC. Methods In this study, GEO, ONCOMINE, GEPIA, cBioPortal, GeneMANIA, Metascape, STRING, TRRUST, and TIMER databases were used to study CXC chemokines. Angiogenesis and T cell killing assay were used to detect the effect of CXCL10 on tumor cell immunity and angiogenesis. Real-time quantitative PCR (qRT-PCR), immunoblotting, and ectopic tumor formation experiments were used to verify the effect of CXCL10 on ovarian cancer tumors. Results We found that CXCL1, CXCL10, CXCL11, CXCL13, and CXCL14 were significantly upregulated in OC samples compared with normal tissues. Our data showed that there was a relationship between the expression of CXC chemokines and the infiltration of six types of immune cells significant correlation. In vitro assay confirmed that overexpression of CXCL10 could enhance the killing effect of T cells and inhibit angiogenesis. Further in vivo assay had shown that CXCL10 could affect the progression of ovarian cancer by increasing the expression of cytotoxic T cells and inhibiting angiogenesis. Conclusion In conclusion, we hope that our data will provide new insights into the development of immunotherapy and the selection of prognostic markers for patients with OC.https://doi.org/10.1186/s12957-021-02440-xOvarian cancerChemokinesAngiogenesisImmune infiltrationBioinformatics |
| spellingShingle | Weiyuan Li Ji-Ao Ma Xun Sheng Chunjie Xiao Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10 World Journal of Surgical Oncology Ovarian cancer Chemokines Angiogenesis Immune infiltration Bioinformatics |
| title | Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10 |
| title_full | Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10 |
| title_fullStr | Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10 |
| title_full_unstemmed | Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10 |
| title_short | Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10 |
| title_sort | screening of cxc chemokines in the microenvironment of ovarian cancer and the biological function of cxcl10 |
| topic | Ovarian cancer Chemokines Angiogenesis Immune infiltration Bioinformatics |
| url | https://doi.org/10.1186/s12957-021-02440-x |
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